Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide
Scorpion peptides have good therapeutic effect on chronic ulcer of diabetic foot, but the related pharmacological mechanism has remained unclear. The different proteins and bacteria present in ulcer exudates from chronic diabetic foot patients, treated with scorpion antimicrobial peptide at differen...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2022/5852786 |
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| author | Zhixiang Tan Zhiguo Yu Xiaobo Xu Lanxia Meng Mosheng Yu Rui Tao Yingliang Wu Zhanyong Zhu |
| author_facet | Zhixiang Tan Zhiguo Yu Xiaobo Xu Lanxia Meng Mosheng Yu Rui Tao Yingliang Wu Zhanyong Zhu |
| author_sort | Zhixiang Tan |
| collection | DOAJ |
| description | Scorpion peptides have good therapeutic effect on chronic ulcer of diabetic foot, but the related pharmacological mechanism has remained unclear. The different proteins and bacteria present in ulcer exudates from chronic diabetic foot patients, treated with scorpion antimicrobial peptide at different stages, were analyzed using isobaric tags for quantification-labeled proteomics and bacteriological methods. According to the mass spectrometry data, a total of 1865 proteins were identified qualitatively, and the number of the different proteins was 130 (mid/early), 401 (late/early), and 310 (mid, late/early). In addition, functional annotation, cluster analysis of effects and the analysis of signal pathway, transcription regulation, and protein-protein interaction network were carried out. The results showed that the biochemical changes of wound microenvironment during the treatment involved activated biological functions such as protein synthesis, cell proliferation, differentiation, migration, movement, and survival. Inhibited biological functions such as cell death, inflammatory response, immune diseases, and bacterial growth were also involved. Bacteriological analysis showed that Burkholderia cepacia was the main bacteria in the early and middle stage of ulcer exudate and Staphylococcus epidermidis in the late stage. This study provides basic data for further elucidation of the molecular mechanism of diabetic foot. |
| format | Article |
| id | doaj-art-a2f1afcd991e49b88d46e067796f433c |
| institution | Kabale University |
| issn | 1466-1861 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a2f1afcd991e49b88d46e067796f433c2025-02-03T01:01:21ZengWileyMediators of Inflammation1466-18612022-01-01202210.1155/2022/5852786Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial PeptideZhixiang Tan0Zhiguo Yu1Xiaobo Xu2Lanxia Meng3Mosheng Yu4Rui Tao5Yingliang Wu6Zhanyong Zhu7Department of Plastic SurgeryDepartment of EmergencyState Key Laboratory of VirologyDepartment of NeurologyDepartment of Plastic SurgeryDepartment of Plastic SurgeryState Key Laboratory of VirologyDepartment of Plastic SurgeryScorpion peptides have good therapeutic effect on chronic ulcer of diabetic foot, but the related pharmacological mechanism has remained unclear. The different proteins and bacteria present in ulcer exudates from chronic diabetic foot patients, treated with scorpion antimicrobial peptide at different stages, were analyzed using isobaric tags for quantification-labeled proteomics and bacteriological methods. According to the mass spectrometry data, a total of 1865 proteins were identified qualitatively, and the number of the different proteins was 130 (mid/early), 401 (late/early), and 310 (mid, late/early). In addition, functional annotation, cluster analysis of effects and the analysis of signal pathway, transcription regulation, and protein-protein interaction network were carried out. The results showed that the biochemical changes of wound microenvironment during the treatment involved activated biological functions such as protein synthesis, cell proliferation, differentiation, migration, movement, and survival. Inhibited biological functions such as cell death, inflammatory response, immune diseases, and bacterial growth were also involved. Bacteriological analysis showed that Burkholderia cepacia was the main bacteria in the early and middle stage of ulcer exudate and Staphylococcus epidermidis in the late stage. This study provides basic data for further elucidation of the molecular mechanism of diabetic foot.http://dx.doi.org/10.1155/2022/5852786 |
| spellingShingle | Zhixiang Tan Zhiguo Yu Xiaobo Xu Lanxia Meng Mosheng Yu Rui Tao Yingliang Wu Zhanyong Zhu Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide Mediators of Inflammation |
| title | Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide |
| title_full | Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide |
| title_fullStr | Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide |
| title_full_unstemmed | Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide |
| title_short | Proteomic Analysis of Exudates from Chronic Ulcer of Diabetic Foot Treated with Scorpion Antimicrobial Peptide |
| title_sort | proteomic analysis of exudates from chronic ulcer of diabetic foot treated with scorpion antimicrobial peptide |
| url | http://dx.doi.org/10.1155/2022/5852786 |
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