Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai
Introduction: Antimicrobial resistance of Salmonella poses a key threat to public health worldwide. Salmonella Enteritidis (S. Enteritidis) (119 isolates) from children under 10 years old with diarrhea in Shanghai from 2010-2012 were characterized for antimicrobial susceptibility, virulence gene pr...
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The Journal of Infection in Developing Countries
2018-07-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9733 |
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| author | Li Xu Xiujuan Zhou Xuebin Xu Karl R Matthews Yue Liu Dai Kuang Xianming Shi |
| author_facet | Li Xu Xiujuan Zhou Xuebin Xu Karl R Matthews Yue Liu Dai Kuang Xianming Shi |
| author_sort | Li Xu |
| collection | DOAJ |
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Introduction: Antimicrobial resistance of Salmonella poses a key threat to public health worldwide. Salmonella Enteritidis (S. Enteritidis) (119 isolates) from children under 10 years old with diarrhea in Shanghai from 2010-2012 were characterized for antimicrobial susceptibility, virulence gene profiles and pulsed field gel electrophoresis (PFGE) patterns.
Methodology: The minimum inhibitory concentration for the 119 S. Enteritidis isolates was determined using an agar dilution method. The presence of virulence genes was detected by polymerase chain reaction (PCR). All the isolates with antimicrobial resistance were subjected to PFGE analysis.
Results: Among these isolates, 71.4% (85) were resistant to sulfafurazole, 59.7% (71) were resistant to ampicillin, 47.1% (56) were resistant to streptomycin, 7.6% (9) were resistant to ceftiofur and 3.4% (4) were resistant to ceftriaxone. Multidrug resistance (MDR) was found in 63.9% (76) of the isolates; 23 resistance profiles were identified. All isolates harbored the ssaQ and sopE virulence genes in the 16 virulence profiles (VPs); VP1 accounted for 70.59% of the 119 isolates. There were 57 PFGE patterns among the 92 isolates tested, mainly grouped into five clusters (A to E). All of the 76 MDR isolates carried multiple virulence genes.
Conclusions: Our study provides useful microbiological data for the successful treatment of S. Enteritidis infections in Shanghai. Although broad spectrum antimicrobials may be useful in the treatment of invasive S. Enteritidis infections, clinicians need to be aware of common microbiological traits, because of the high prevalence of MDR.
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| format | Article |
| id | doaj-art-a2d2b64adf0d4b41b4d2fe4f1376e325 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-a2d2b64adf0d4b41b4d2fe4f1376e3252025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-07-01120710.3855/jidc.9733Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in ShanghaiLi Xu0Xiujuan Zhou1Xuebin Xu2Karl R Matthews3Yue Liu4Dai Kuang5Xianming Shi6Shanghai Jiao Tong University, Shanghai, ChinaShanghai Jiao Tong University, Shanghai, ChinaShanghai Center for Disease Control and Prevention, Shanghai, ChinaRutgers, The State University of New Jersey, New Brunswick, NJ, United StatesShanghai Jiao Tong University, Shanghai, ChinaShanghai Jiao Tong UniversityShanghai Jiao Tong University, Shanghai, China Introduction: Antimicrobial resistance of Salmonella poses a key threat to public health worldwide. Salmonella Enteritidis (S. Enteritidis) (119 isolates) from children under 10 years old with diarrhea in Shanghai from 2010-2012 were characterized for antimicrobial susceptibility, virulence gene profiles and pulsed field gel electrophoresis (PFGE) patterns. Methodology: The minimum inhibitory concentration for the 119 S. Enteritidis isolates was determined using an agar dilution method. The presence of virulence genes was detected by polymerase chain reaction (PCR). All the isolates with antimicrobial resistance were subjected to PFGE analysis. Results: Among these isolates, 71.4% (85) were resistant to sulfafurazole, 59.7% (71) were resistant to ampicillin, 47.1% (56) were resistant to streptomycin, 7.6% (9) were resistant to ceftiofur and 3.4% (4) were resistant to ceftriaxone. Multidrug resistance (MDR) was found in 63.9% (76) of the isolates; 23 resistance profiles were identified. All isolates harbored the ssaQ and sopE virulence genes in the 16 virulence profiles (VPs); VP1 accounted for 70.59% of the 119 isolates. There were 57 PFGE patterns among the 92 isolates tested, mainly grouped into five clusters (A to E). All of the 76 MDR isolates carried multiple virulence genes. Conclusions: Our study provides useful microbiological data for the successful treatment of S. Enteritidis infections in Shanghai. Although broad spectrum antimicrobials may be useful in the treatment of invasive S. Enteritidis infections, clinicians need to be aware of common microbiological traits, because of the high prevalence of MDR. https://jidc.org/index.php/journal/article/view/9733S. enteridisantimicrobial resistancevirulence genePFGEchildhood infections |
| spellingShingle | Li Xu Xiujuan Zhou Xuebin Xu Karl R Matthews Yue Liu Dai Kuang Xianming Shi Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai Journal of Infection in Developing Countries S. enteridis antimicrobial resistance virulence gene PFGE childhood infections |
| title | Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai |
| title_full | Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai |
| title_fullStr | Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai |
| title_full_unstemmed | Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai |
| title_short | Antimicrobial resistance, virulence genes and molecular subtypes of S. Enteritidis isolated from children in Shanghai |
| title_sort | antimicrobial resistance virulence genes and molecular subtypes of s enteritidis isolated from children in shanghai |
| topic | S. enteridis antimicrobial resistance virulence gene PFGE childhood infections |
| url | https://jidc.org/index.php/journal/article/view/9733 |
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