Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes
Reactive oxygen species generated in dermal cells of human skin is related to skin disorders or diseases. In this study, Raman analysis effectively clarified the identities of three types of human skin models after the models were stimulated with hydrogen peroxide. With the Caucasian skin model, the...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Journal of Spectroscopy |
| Online Access: | http://dx.doi.org/10.1155/2013/903450 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567117876035584 |
|---|---|
| author | Shin-ichi Morita Sota Takanezawa Akira Date Shin Watanabe Yasushi Sako |
| author_facet | Shin-ichi Morita Sota Takanezawa Akira Date Shin Watanabe Yasushi Sako |
| author_sort | Shin-ichi Morita |
| collection | DOAJ |
| description | Reactive oxygen species generated in dermal cells of human skin is related to skin disorders or diseases. In this study, Raman analysis effectively clarified the identities of three types of human skin models after the models were stimulated with hydrogen peroxide. With the Caucasian skin model, the major Raman bands underwent large intensity changes within 4-5 days of stimulation. With the Black skin model, the Raman bands remained almost unchanged. The changes in the Asian skin model were unique compared to those in the above two. Eumelanin and pheomelanin are probably the main compounds that differentiate dermal cells in terms of their sensitivity to hydrogen peroxide. |
| format | Article |
| id | doaj-art-a2cfed9353594868b819d1099dacbc51 |
| institution | Kabale University |
| issn | 2314-4920 2314-4939 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Spectroscopy |
| spelling | doaj-art-a2cfed9353594868b819d1099dacbc512025-02-03T01:02:25ZengWileyJournal of Spectroscopy2314-49202314-49392013-01-01201310.1155/2013/903450903450Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian MelanocytesShin-ichi Morita0Sota Takanezawa1Akira Date2Shin Watanabe3Yasushi Sako4Cellular Informatics Laboratory, Advanced Science Institute, RIKEN, Wako 351-0198, JapanCellular Informatics Laboratory, Advanced Science Institute, RIKEN, Wako 351-0198, JapanKobe Technical Center, Procter and Gamble Japan, Kobe 658-0032, JapanProcter & Gamble International Operations S.A., Singapore Branch, Immunos No. 02-12, 8A, Biomedical Grove, SingaporeCellular Informatics Laboratory, Advanced Science Institute, RIKEN, Wako 351-0198, JapanReactive oxygen species generated in dermal cells of human skin is related to skin disorders or diseases. In this study, Raman analysis effectively clarified the identities of three types of human skin models after the models were stimulated with hydrogen peroxide. With the Caucasian skin model, the major Raman bands underwent large intensity changes within 4-5 days of stimulation. With the Black skin model, the Raman bands remained almost unchanged. The changes in the Asian skin model were unique compared to those in the above two. Eumelanin and pheomelanin are probably the main compounds that differentiate dermal cells in terms of their sensitivity to hydrogen peroxide.http://dx.doi.org/10.1155/2013/903450 |
| spellingShingle | Shin-ichi Morita Sota Takanezawa Akira Date Shin Watanabe Yasushi Sako Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes Journal of Spectroscopy |
| title | Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes |
| title_full | Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes |
| title_fullStr | Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes |
| title_full_unstemmed | Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes |
| title_short | Raman Spectroscopic Analysis of H2O2-Stimulated Three-Dimensional Human Skin Models Containing Asian, Black, and Caucasian Melanocytes |
| title_sort | raman spectroscopic analysis of h2o2 stimulated three dimensional human skin models containing asian black and caucasian melanocytes |
| url | http://dx.doi.org/10.1155/2013/903450 |
| work_keys_str_mv | AT shinichimorita ramanspectroscopicanalysisofh2o2stimulatedthreedimensionalhumanskinmodelscontainingasianblackandcaucasianmelanocytes AT sotatakanezawa ramanspectroscopicanalysisofh2o2stimulatedthreedimensionalhumanskinmodelscontainingasianblackandcaucasianmelanocytes AT akiradate ramanspectroscopicanalysisofh2o2stimulatedthreedimensionalhumanskinmodelscontainingasianblackandcaucasianmelanocytes AT shinwatanabe ramanspectroscopicanalysisofh2o2stimulatedthreedimensionalhumanskinmodelscontainingasianblackandcaucasianmelanocytes AT yasushisako ramanspectroscopicanalysisofh2o2stimulatedthreedimensionalhumanskinmodelscontainingasianblackandcaucasianmelanocytes |