Cryopreservation as a versatile strategy for the construction and application of organoids
Organoids are three-dimensional structures derived from stem cells that recapitulate the gene expression profiles and functional characteristics of their tissue of origin, rendering them invaluable tools for disease modeling, drug screening, and precision medicine. Despite their promise, the widespr...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2025-08-01
|
| Series: | Frigid Zone Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/fzm-2025-0013 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849225118696341504 |
|---|---|
| author | Wang Xinyue Han Hengxin Xu Yi |
| author_facet | Wang Xinyue Han Hengxin Xu Yi |
| author_sort | Wang Xinyue |
| collection | DOAJ |
| description | Organoids are three-dimensional structures derived from stem cells that recapitulate the gene expression profiles and functional characteristics of their tissue of origin, rendering them invaluable tools for disease modeling, drug screening, and precision medicine. Despite their promise, the widespread application of organoids is limited by extended culture durations and technical complexity. Cryopreservation has emerged as a critical strategy to overcome challenges related to the long-term storage and application of organoids, offering a range of preservation approaches tailored to organoid development. Nevertheless, conventional cryopreservation techniques encounter significant limitations when applied to organoids. To address these issues, the development of naturally derived, low-toxicity Cryoprotectants (CPAs), along with the optimization of CPA loading methods and refinement of cooling and warming protocols, is essential to mitigate cryoinjury. Looking forward, the comprehensive enhancement of cryopreservation technologies may facilitate the transformation of organoids into “off-the-shelf” products, enabling scalable production, batch standardization, and centralized distribution. Such advancements will lay the foundation for the establishment of Next-Generation Living Biobanks (NGLB). |
| format | Article |
| id | doaj-art-a2bd7b485cbc44acb4e6c0350ff28ea9 |
| institution | Kabale University |
| issn | 2719-8073 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Frigid Zone Medicine |
| spelling | doaj-art-a2bd7b485cbc44acb4e6c0350ff28ea92025-08-25T06:10:33ZengDe GruyterFrigid Zone Medicine2719-80732025-08-015211912310.1515/fzm-2025-0013Cryopreservation as a versatile strategy for the construction and application of organoidsWang Xinyue0Han Hengxin1Xu Yi2Institute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai200093, ChinaInstitute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai200093, ChinaInstitute of Biothermal Science & Technology, University of Shanghai for Science and Technology, Shanghai200093, ChinaOrganoids are three-dimensional structures derived from stem cells that recapitulate the gene expression profiles and functional characteristics of their tissue of origin, rendering them invaluable tools for disease modeling, drug screening, and precision medicine. Despite their promise, the widespread application of organoids is limited by extended culture durations and technical complexity. Cryopreservation has emerged as a critical strategy to overcome challenges related to the long-term storage and application of organoids, offering a range of preservation approaches tailored to organoid development. Nevertheless, conventional cryopreservation techniques encounter significant limitations when applied to organoids. To address these issues, the development of naturally derived, low-toxicity Cryoprotectants (CPAs), along with the optimization of CPA loading methods and refinement of cooling and warming protocols, is essential to mitigate cryoinjury. Looking forward, the comprehensive enhancement of cryopreservation technologies may facilitate the transformation of organoids into “off-the-shelf” products, enabling scalable production, batch standardization, and centralized distribution. Such advancements will lay the foundation for the establishment of Next-Generation Living Biobanks (NGLB).https://doi.org/10.1515/fzm-2025-0013organoidstumor tissuescryopreservationnext-generation living biobanks |
| spellingShingle | Wang Xinyue Han Hengxin Xu Yi Cryopreservation as a versatile strategy for the construction and application of organoids Frigid Zone Medicine organoids tumor tissues cryopreservation next-generation living biobanks |
| title | Cryopreservation as a versatile strategy for the construction and application of organoids |
| title_full | Cryopreservation as a versatile strategy for the construction and application of organoids |
| title_fullStr | Cryopreservation as a versatile strategy for the construction and application of organoids |
| title_full_unstemmed | Cryopreservation as a versatile strategy for the construction and application of organoids |
| title_short | Cryopreservation as a versatile strategy for the construction and application of organoids |
| title_sort | cryopreservation as a versatile strategy for the construction and application of organoids |
| topic | organoids tumor tissues cryopreservation next-generation living biobanks |
| url | https://doi.org/10.1515/fzm-2025-0013 |
| work_keys_str_mv | AT wangxinyue cryopreservationasaversatilestrategyfortheconstructionandapplicationoforganoids AT hanhengxin cryopreservationasaversatilestrategyfortheconstructionandapplicationoforganoids AT xuyi cryopreservationasaversatilestrategyfortheconstructionandapplicationoforganoids |