Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon
As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/3625823 |
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| _version_ | 1832552999528955904 |
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| author | Qingxin Liu Jialong Fan Chuang Zhou Liqun Wang Bin Zhao Haibin Zhang Bin Liu Chunyi Tong |
| author_facet | Qingxin Liu Jialong Fan Chuang Zhou Liqun Wang Bin Zhao Haibin Zhang Bin Liu Chunyi Tong |
| author_sort | Qingxin Liu |
| collection | DOAJ |
| description | As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA. |
| format | Article |
| id | doaj-art-a2b2ec1ff997403c9dc7384a183bfb14 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-a2b2ec1ff997403c9dc7384a183bfb142025-02-03T05:57:10ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/36258233625823Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular BeaconQingxin Liu0Jialong Fan1Chuang Zhou2Liqun Wang3Bin Zhao4Haibin Zhang5Bin Liu6Chunyi Tong7College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Biology, Hunan University, Changsha, Hunan 410082, ChinaJiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu 212400, ChinaJiangsu Vocational College of Agriculture and Forestry, Jurong, Jiangsu 212400, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu 210095, ChinaCollege of Biology, Hunan University, Changsha, Hunan 410082, ChinaCollege of Biology, Hunan University, Changsha, Hunan 410082, ChinaAs a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. With the tool of molecular beacons which can hybridize specifically with target miRNA-21 and generate fluorescence signal change, this paper develops a direct, simple, and rapid method for miRNA-21 detection with detection limit of 0.5 nM. Under the optimal conditions, the method was used to detect the expression of miRNA-21 in tumor cells and tissues. The results showed significant differences of miRNA-21 levels in tumor cells which have different origins and different degree of malignancy. In 8 cases of gastric cancer tissues and adjacent tissues, the level of miRNA-21 in 6 cases was higher than that in adjacent tissues, 1 case had lower expression level than that in adjacent tissues, and 1 case had no significant difference. Furthermore, qRT-PCR method was used to verify the detection results based on the fluorescent probe detection method. The consistent results show that the molecular beacon assay has a good prospect in direct and rapid detection of miRNA-21 expression and will be widely used in the functional research and clinical diagnosis of microRNA.http://dx.doi.org/10.1155/2018/3625823 |
| spellingShingle | Qingxin Liu Jialong Fan Chuang Zhou Liqun Wang Bin Zhao Haibin Zhang Bin Liu Chunyi Tong Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon International Journal of Analytical Chemistry |
| title | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
| title_full | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
| title_fullStr | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
| title_full_unstemmed | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
| title_short | Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon |
| title_sort | quantitative detection of mirna 21 expression in tumor cells and tissues based on molecular beacon |
| url | http://dx.doi.org/10.1155/2018/3625823 |
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