High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay
Single nucleotide polymorphisms (SNPs) are the class of DNA variants that are most common in the genome. Technologies are needed that allow relatively highthroughput, high-quality genotyping of SNPs, with rapid assay development for individual SNPs of interest. We have implemented an accurate and hi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
1999-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/99273rr02 |
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| _version_ | 1850152235062788096 |
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| author | Phillip A. Morin Robert Saiz Atousa Monjazeb |
| author_facet | Phillip A. Morin Robert Saiz Atousa Monjazeb |
| author_sort | Phillip A. Morin |
| collection | DOAJ |
| description | Single nucleotide polymorphisms (SNPs) are the class of DNA variants that are most common in the genome. Technologies are needed that allow relatively highthroughput, high-quality genotyping of SNPs, with rapid assay development for individual SNPs of interest. We have implemented an accurate and high-throughput SNP genotyping system using a commercially available fluorescent 5′ exonuclease assay. Optimization of the assay system for low-volume reactions and low-probe concentrations has reduced assay costs by 75%. Using a simple assay optimization process, we successfully developed genotyping assays for 92% of assays attempted (309 out of 335) and have generated over 200 000 genotypes. |
| format | Article |
| id | doaj-art-a292104d97c74c20a17a7da5b8d4e32f |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1999-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-a292104d97c74c20a17a7da5b8d4e32f2025-08-20T02:26:02ZengTaylor & Francis GroupBioTechniques0736-62051940-98181999-09-0127353855210.2144/99273rr02High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease AssayPhillip A. Morin0Robert Saiz1Atousa Monjazeb21Axys Pharmaceuticals, La Jolla, CA, USA1Axys Pharmaceuticals, La Jolla, CA, USA1Axys Pharmaceuticals, La Jolla, CA, USASingle nucleotide polymorphisms (SNPs) are the class of DNA variants that are most common in the genome. Technologies are needed that allow relatively highthroughput, high-quality genotyping of SNPs, with rapid assay development for individual SNPs of interest. We have implemented an accurate and high-throughput SNP genotyping system using a commercially available fluorescent 5′ exonuclease assay. Optimization of the assay system for low-volume reactions and low-probe concentrations has reduced assay costs by 75%. Using a simple assay optimization process, we successfully developed genotyping assays for 92% of assays attempted (309 out of 335) and have generated over 200 000 genotypes.https://www.future-science.com/doi/10.2144/99273rr02 |
| spellingShingle | Phillip A. Morin Robert Saiz Atousa Monjazeb High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay BioTechniques |
| title | High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay |
| title_full | High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay |
| title_fullStr | High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay |
| title_full_unstemmed | High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay |
| title_short | High-Throughput Single Nucleotide Polymorphism Genotyping by Fluorescent 5′ Exonuclease Assay |
| title_sort | high throughput single nucleotide polymorphism genotyping by fluorescent 5 exonuclease assay |
| url | https://www.future-science.com/doi/10.2144/99273rr02 |
| work_keys_str_mv | AT phillipamorin highthroughputsinglenucleotidepolymorphismgenotypingbyfluorescent5exonucleaseassay AT robertsaiz highthroughputsinglenucleotidepolymorphismgenotypingbyfluorescent5exonucleaseassay AT atousamonjazeb highthroughputsinglenucleotidepolymorphismgenotypingbyfluorescent5exonucleaseassay |