RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori
Summary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori. We found that RNase Y is membrane-associated and its ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225000756 |
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| author | Federico D’Agostino Eva Pinatel Alexandra Meynhardt Vincenzo Scarlato Andrea Vannini Davide Roncarati |
| author_facet | Federico D’Agostino Eva Pinatel Alexandra Meynhardt Vincenzo Scarlato Andrea Vannini Davide Roncarati |
| author_sort | Federico D’Agostino |
| collection | DOAJ |
| description | Summary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori. We found that RNase Y is membrane-associated and its expression is controlled during bacterial growth and by Fur in response to iron. We observed that RNase Y deletion has a limited impact on H. pylori transcriptome and on bacterial growth. Interestingly, we found that RNase Y is involved in the metabolism of CncR1, a virulence-associated sRNA oppositely modulating bacterial motility and adhesion to host cells. Indeed, RNase Y inactivation led to the accumulation of a 3′-extended CncR1 isoform, which appeared unable to interact in vitro with a known target mRNA. The observation that the RNAse Y-mutant strain showed deregulation of several members of the CncR1 regulon suggests this ribonuclease has an important role in H. pylori posttranscriptional regulation. |
| format | Article |
| id | doaj-art-a27f71a7e7754a9f99bfb36eefba8351 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-a27f71a7e7754a9f99bfb36eefba83512025-01-31T05:12:12ZengElsevieriScience2589-00422025-02-01282111815RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pyloriFederico D’Agostino0Eva Pinatel1Alexandra Meynhardt2Vincenzo Scarlato3Andrea Vannini4Davide Roncarati5Department of Pharmacy and Biotechnology, Alma Mater Studiorum – University of Bologna, Bologna, ItalyInstitute of Biomedical Technologies - National Research Council, Segrate, ItalyDepartment of Pharmacy and Biotechnology, Alma Mater Studiorum – University of Bologna, Bologna, ItalyDepartment of Pharmacy and Biotechnology, Alma Mater Studiorum – University of Bologna, Bologna, ItalyDepartment of Pharmacy and Biotechnology, Alma Mater Studiorum – University of Bologna, Bologna, Italy; Corresponding authorDepartment of Pharmacy and Biotechnology, Alma Mater Studiorum – University of Bologna, Bologna, Italy; Corresponding authorSummary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori. We found that RNase Y is membrane-associated and its expression is controlled during bacterial growth and by Fur in response to iron. We observed that RNase Y deletion has a limited impact on H. pylori transcriptome and on bacterial growth. Interestingly, we found that RNase Y is involved in the metabolism of CncR1, a virulence-associated sRNA oppositely modulating bacterial motility and adhesion to host cells. Indeed, RNase Y inactivation led to the accumulation of a 3′-extended CncR1 isoform, which appeared unable to interact in vitro with a known target mRNA. The observation that the RNAse Y-mutant strain showed deregulation of several members of the CncR1 regulon suggests this ribonuclease has an important role in H. pylori posttranscriptional regulation.http://www.sciencedirect.com/science/article/pii/S2589004225000756Human metabolismCell biology |
| spellingShingle | Federico D’Agostino Eva Pinatel Alexandra Meynhardt Vincenzo Scarlato Andrea Vannini Davide Roncarati RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori iScience Human metabolism Cell biology |
| title | RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori |
| title_full | RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori |
| title_fullStr | RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori |
| title_full_unstemmed | RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori |
| title_short | RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori |
| title_sort | rnase y mediates posttranscriptional control of the virulence associated cncr1 small rna in helicobacter pylori |
| topic | Human metabolism Cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225000756 |
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