Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System

The coating of an active drug, 6-mercaptopurine, into the iron oxide nanoparticles-polyethylene glycol (FNPs-PEG) in order to form a new nanocomposite, FPEGMP-2, was accomplished using coprecipitation technique. The resulting nanosized with a narrow size distribution magnetic polymeric particles sho...

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Main Authors: Dena Dorniani, Aminu Umar Kura, Samer Hasan Hussein-Al-Ali, Mohd Zobir bin Hussein, Sharida Fakurazi, Abdul Halim Shaari, Zalinah Ahmad
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2014/972501
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author Dena Dorniani
Aminu Umar Kura
Samer Hasan Hussein-Al-Ali
Mohd Zobir bin Hussein
Sharida Fakurazi
Abdul Halim Shaari
Zalinah Ahmad
author_facet Dena Dorniani
Aminu Umar Kura
Samer Hasan Hussein-Al-Ali
Mohd Zobir bin Hussein
Sharida Fakurazi
Abdul Halim Shaari
Zalinah Ahmad
author_sort Dena Dorniani
collection DOAJ
description The coating of an active drug, 6-mercaptopurine, into the iron oxide nanoparticles-polyethylene glycol (FNPs-PEG) in order to form a new nanocomposite, FPEGMP-2, was accomplished using coprecipitation technique. The resulting nanosized with a narrow size distribution magnetic polymeric particles show the superparamagnetic properties with 38.6 emu/g saturation magnetization at room temperature. Fourier transform infrared spectroscopy and the thermal analysis study supported the formation of the nanocomposite and the enhancement of thermal stability in the resulting nanocomposite comparing with its counterpart in free state. The loading of 6-mercaptopurine (MP) in the FPEGMP-2 nanocomposite was estimated to be about 5.6% and the kinetic experimental data properly correlated with the pseudo-second order model. Also, the release of MP from the FPEGMP-2 nanocomposite shows the sustained release manner which is remarkably lower in phosphate buffered solution at pH 7.4 than pH 4.8, due to different release mechanism. The maximum percentage release of MP from the nanocomposite reached about 60% and 97% within about 92 and 74 hours when exposed to pH 7.4 and 4.8, respectively.
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institution Kabale University
issn 2356-6140
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language English
publishDate 2014-01-01
publisher Wiley
record_format Article
series The Scientific World Journal
spelling doaj-art-a27e61d1ebba41d6a1b98ef92258f7d82025-08-20T03:38:44ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/972501972501Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery SystemDena Dorniani0Aminu Umar Kura1Samer Hasan Hussein-Al-Ali2Mohd Zobir bin Hussein3Sharida Fakurazi4Abdul Halim Shaari5Zalinah Ahmad6Materials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaVaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLaboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaMaterials Synthesis and Characterization Laboratory (MSCL), Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaVaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaPhysics Department, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaVaccines and Immunotherapeutics Laboratory (IBS), Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaThe coating of an active drug, 6-mercaptopurine, into the iron oxide nanoparticles-polyethylene glycol (FNPs-PEG) in order to form a new nanocomposite, FPEGMP-2, was accomplished using coprecipitation technique. The resulting nanosized with a narrow size distribution magnetic polymeric particles show the superparamagnetic properties with 38.6 emu/g saturation magnetization at room temperature. Fourier transform infrared spectroscopy and the thermal analysis study supported the formation of the nanocomposite and the enhancement of thermal stability in the resulting nanocomposite comparing with its counterpart in free state. The loading of 6-mercaptopurine (MP) in the FPEGMP-2 nanocomposite was estimated to be about 5.6% and the kinetic experimental data properly correlated with the pseudo-second order model. Also, the release of MP from the FPEGMP-2 nanocomposite shows the sustained release manner which is remarkably lower in phosphate buffered solution at pH 7.4 than pH 4.8, due to different release mechanism. The maximum percentage release of MP from the nanocomposite reached about 60% and 97% within about 92 and 74 hours when exposed to pH 7.4 and 4.8, respectively.http://dx.doi.org/10.1155/2014/972501
spellingShingle Dena Dorniani
Aminu Umar Kura
Samer Hasan Hussein-Al-Ali
Mohd Zobir bin Hussein
Sharida Fakurazi
Abdul Halim Shaari
Zalinah Ahmad
Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
The Scientific World Journal
title Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
title_full Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
title_fullStr Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
title_full_unstemmed Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
title_short Release Behavior and Toxicity Profiles towards Leukemia (WEHI-3B) Cell Lines of 6-Mercaptopurine-PEG-Coated Magnetite Nanoparticles Delivery System
title_sort release behavior and toxicity profiles towards leukemia wehi 3b cell lines of 6 mercaptopurine peg coated magnetite nanoparticles delivery system
url http://dx.doi.org/10.1155/2014/972501
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