<i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential
The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including <i>Klebsiella pneumoniae</i>. <i>Klebsiella&...
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2025-01-01
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| author | Lika Leshkasheli Ia Kusradze Darejan Bolkvadze Lia Askilashvili Maria Chichashvili Giorgi Tsertsvadze Elisabed Zaldastanishvili |
| author_facet | Lika Leshkasheli Ia Kusradze Darejan Bolkvadze Lia Askilashvili Maria Chichashvili Giorgi Tsertsvadze Elisabed Zaldastanishvili |
| author_sort | Lika Leshkasheli |
| collection | DOAJ |
| description | The rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including <i>Klebsiella pneumoniae</i>. <i>Klebsiella</i>-targeting phages are among the most promising alternative therapy options. They have already been successfully applied in a number of cases, and it is expected that the need for anti-<i>Klebsiella</i> phages will only increase in the future. This prospect highlights the need for well-characterized therapeutic phages. In this work, we describe a <i>K. pneumoniae</i> phage, which also infects strains of <i>Klebsiella oxytoca</i>. Here, we characterize phage M198 in terms of its biological and genetic properties. Since in some phage therapy cases, phages are administered in combination with antibiotics, here, we also screen for possible synergistic effects of combining phage M198 with six different antibiotics. We found that phage M198 has good lytic activity against clinical isolates; it does not have any indications of a temperate lifestyle, and it has synergistic potential when combined with some therapeutically relevant antibiotics. |
| format | Article |
| id | doaj-art-a27c50fd50864b79891962a38a39469e |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-a27c50fd50864b79891962a38a39469e2025-01-24T13:52:38ZengMDPI AGViruses1999-49152025-01-0117111510.3390/v17010115<i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic PotentialLika Leshkasheli0Ia Kusradze1Darejan Bolkvadze2Lia Askilashvili3Maria Chichashvili4Giorgi Tsertsvadze5Elisabed Zaldastanishvili6Laboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaLaboratory of General Microbiology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaLaboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaLaboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaLaboratory of General Microbiology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaElectron Microscopy Unit, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaLaboratory of Molecular Biology, G. Eliava Institute of Bacteriophages, Microbiology and Virology, 0160 Tbilisi, GeorgiaThe rapid worldwide spread of antibiotic resistance is quickly becoming an increasingly concerning problem for human healthcare. Non-antibiotic antibacterial agents are in high demand for many Gram-negative bacterial pathogens, including <i>Klebsiella pneumoniae</i>. <i>Klebsiella</i>-targeting phages are among the most promising alternative therapy options. They have already been successfully applied in a number of cases, and it is expected that the need for anti-<i>Klebsiella</i> phages will only increase in the future. This prospect highlights the need for well-characterized therapeutic phages. In this work, we describe a <i>K. pneumoniae</i> phage, which also infects strains of <i>Klebsiella oxytoca</i>. Here, we characterize phage M198 in terms of its biological and genetic properties. Since in some phage therapy cases, phages are administered in combination with antibiotics, here, we also screen for possible synergistic effects of combining phage M198 with six different antibiotics. We found that phage M198 has good lytic activity against clinical isolates; it does not have any indications of a temperate lifestyle, and it has synergistic potential when combined with some therapeutically relevant antibiotics.https://www.mdpi.com/1999-4915/17/1/115<i>Klebsiella pneumoniae</i>phageantibioticssynergy |
| spellingShingle | Lika Leshkasheli Ia Kusradze Darejan Bolkvadze Lia Askilashvili Maria Chichashvili Giorgi Tsertsvadze Elisabed Zaldastanishvili <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential Viruses <i>Klebsiella pneumoniae</i> phage antibiotics synergy |
| title | <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential |
| title_full | <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential |
| title_fullStr | <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential |
| title_full_unstemmed | <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential |
| title_short | <i>Klebsiella pneumoniae</i> Phage M198 and Its Therapeutic Potential |
| title_sort | i klebsiella pneumoniae i phage m198 and its therapeutic potential |
| topic | <i>Klebsiella pneumoniae</i> phage antibiotics synergy |
| url | https://www.mdpi.com/1999-4915/17/1/115 |
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