A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples.

A modified RNA-Seq approach for whole genome sequencing of RNA viruses from faecal and blood samples.

To date, very large scale sequencing of many clinically important RNA viruses has been complicated by their high population molecular variation, which creates challenges for polymerase chain reaction and sequencing primer design. Many RNA viruses are also difficult or currently not possible to cultu...

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Main Authors: Elizabeth M Batty, T H Nicholas Wong, Amy Trebes, Karène Argoud, Moustafa Attar, David Buck, Camilla L C Ip, Tanya Golubchik, Madeleine Cule, Rory Bowden, Charis Manganis, Paul Klenerman, Eleanor Barnes, A Sarah Walker, David H Wyllie, Daniel J Wilson, Kate E Dingle, Tim E A Peto, Derrick W Crook, Paolo Piazza
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0066129&type=printable
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