Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions
We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/194723 |
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| _version_ | 1832549812753399808 |
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| author | Clara Di Filippo Pasquale Petronella Fulvio Freda Marco Scorzelli Marco Ferretti Sivestro Canonico Francesco Rossi Michele D'Amico |
| author_facet | Clara Di Filippo Pasquale Petronella Fulvio Freda Marco Scorzelli Marco Ferretti Sivestro Canonico Francesco Rossi Michele D'Amico |
| author_sort | Clara Di Filippo |
| collection | DOAJ |
| description | We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions.
We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue.
The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats. |
| format | Article |
| id | doaj-art-a21d692bb2594fc4bdcbefb738f56af7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a21d692bb2594fc4bdcbefb738f56af72025-02-03T06:08:34ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/194723194723Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal AdhesionsClara Di Filippo0Pasquale Petronella1Fulvio Freda2Marco Scorzelli3Marco Ferretti4Sivestro Canonico5Francesco Rossi6Michele D'Amico7Department of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, 80138 Naples, ItalyDepartment of Gerontology, Geriatric and Metabolic Diseases, Second University of Naples, 80138 Naples, ItalyDepartment of Gerontology, Geriatric and Metabolic Diseases, Second University of Naples, 80138 Naples, ItalyDepartment of Gerontology, Geriatric and Metabolic Diseases, Second University of Naples, 80138 Naples, ItalyDepartment of Gerontology, Geriatric and Metabolic Diseases, Second University of Naples, 80138 Naples, ItalyDepartment of Gerontology, Geriatric and Metabolic Diseases, Second University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, Second University of Naples, 80138 Naples, ItalyWe investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions. We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue. The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats.http://dx.doi.org/10.1155/2012/194723 |
| spellingShingle | Clara Di Filippo Pasquale Petronella Fulvio Freda Marco Scorzelli Marco Ferretti Sivestro Canonico Francesco Rossi Michele D'Amico Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions Mediators of Inflammation |
| title | Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions |
| title_full | Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions |
| title_fullStr | Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions |
| title_full_unstemmed | Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions |
| title_short | Involvement of the Ubiquitin-Proteasome System in the Formation of Experimental Postsurgical Peritoneal Adhesions |
| title_sort | involvement of the ubiquitin proteasome system in the formation of experimental postsurgical peritoneal adhesions |
| url | http://dx.doi.org/10.1155/2012/194723 |
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