Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection
Background. The restoration of auricular cartilage is a major problem of otolaryngology. The low regenerative capacity of cartilage requires alternative approaches such as cell and tissue engineering. Stem cells are one of the ways to repair auricular cartilage damages. The aim of the investigation...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Vilnius University Press
2023-11-01
|
| Series: | Acta Medica Lituanica |
| Subjects: | |
| Online Access: | https://www.journals.vu.lt/AML/article/view/32971 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593387462590464 |
|---|---|
| author | Pavlo Virych Nadiia Shuvalova Anton Karas Galina Karas Svitlana Chaika Tetiana Kucherenko Ganna Minina Marina Timchenko Oleg Melnykov Yurii Minin |
| author_facet | Pavlo Virych Nadiia Shuvalova Anton Karas Galina Karas Svitlana Chaika Tetiana Kucherenko Ganna Minina Marina Timchenko Oleg Melnykov Yurii Minin |
| author_sort | Pavlo Virych |
| collection | DOAJ |
| description |
Background. The restoration of auricular cartilage is a major problem of otolaryngology. The low regenerative capacity of cartilage requires alternative approaches such as cell and tissue engineering. Stem cells are one of the ways to repair auricular cartilage damages. The aim of the investigation was the regeneration of an artificial defect of the auricular cartilage of rabbits after the intravenous injection of stem cells.
Materials and Methods. The study was carried out on rabbits. A narrow strip of auricular cartilage was surgically removed. A previously prepared suspension of homologous mesenchymal stem cells (5 million) in 0.5 ml physiological solution was injected into the vein of the opposite ear. Tissue samples from the site of the injury were collected after 1, 2, and 3 months. Histological examinations of the tissues were carried out after staining with fuchsin-eosin, azure II–eosin, and according to Weigert. In addition, the amount of interleukin-6 (IL-6) and the transforming growth factor β1 (TGF-β1) in the blood serum were determined.
Results. The main method of healing is the formation of a connective tissue scar. Yret, an increase of the number of fibroblasts and single islands of the newly formed auricular cartilage was found, which indicates the migration of the injected stem cells to the site of the damage and settling there. The intravenous injection of stem cells did not affect the secretion of pro-inflammatory IL-6, but significantly increased the amount of TGF-β1.
Conclusions. We assume that regenerative processes were stimulated. Nevertheless, they were aimed at quickly restoring the tissue integrity through the typical stages of scar formation. The restoration of cartilage integrity requires additional regulatory factors which will determine the chondrogenic differentiation of stem cells.
|
| format | Article |
| id | doaj-art-a20d7e7570544588bd4287e84fd2ca10 |
| institution | Kabale University |
| issn | 1392-0138 2029-4174 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Vilnius University Press |
| record_format | Article |
| series | Acta Medica Lituanica |
| spelling | doaj-art-a20d7e7570544588bd4287e84fd2ca102025-01-20T18:08:04ZengVilnius University PressActa Medica Lituanica1392-01382029-41742023-11-0130210.15388/Amed.2023.30.2.15Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell InjectionPavlo Virych0https://orcid.org/0000-0002-1463-1992Nadiia Shuvalova1https://orcid.org/0000-0002-6390-5996Anton Karas2https://orcid.org/0000-0003-2324-780XGalina Karas3https://orcid.org/0000-0003-3098-3813Svitlana Chaika4https://orcid.org/0000-0003-4324-5529Tetiana Kucherenko5https://orcid.org/0000-0002-2158-8101Ganna Minina6Marina Timchenko7Oleg Melnykov8Yurii Minin9SI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, UkraineSI “O.S. Kolomiychenko Institute of Otolaryngology of National Academy of Medical Sciences of Ukraine”, Ukraine Background. The restoration of auricular cartilage is a major problem of otolaryngology. The low regenerative capacity of cartilage requires alternative approaches such as cell and tissue engineering. Stem cells are one of the ways to repair auricular cartilage damages. The aim of the investigation was the regeneration of an artificial defect of the auricular cartilage of rabbits after the intravenous injection of stem cells. Materials and Methods. The study was carried out on rabbits. A narrow strip of auricular cartilage was surgically removed. A previously prepared suspension of homologous mesenchymal stem cells (5 million) in 0.5 ml physiological solution was injected into the vein of the opposite ear. Tissue samples from the site of the injury were collected after 1, 2, and 3 months. Histological examinations of the tissues were carried out after staining with fuchsin-eosin, azure II–eosin, and according to Weigert. In addition, the amount of interleukin-6 (IL-6) and the transforming growth factor β1 (TGF-β1) in the blood serum were determined. Results. The main method of healing is the formation of a connective tissue scar. Yret, an increase of the number of fibroblasts and single islands of the newly formed auricular cartilage was found, which indicates the migration of the injected stem cells to the site of the damage and settling there. The intravenous injection of stem cells did not affect the secretion of pro-inflammatory IL-6, but significantly increased the amount of TGF-β1. Conclusions. We assume that regenerative processes were stimulated. Nevertheless, they were aimed at quickly restoring the tissue integrity through the typical stages of scar formation. The restoration of cartilage integrity requires additional regulatory factors which will determine the chondrogenic differentiation of stem cells. https://www.journals.vu.lt/AML/article/view/32971auricular cartilagestem cellsregenerationinflammationintercellular matrix |
| spellingShingle | Pavlo Virych Nadiia Shuvalova Anton Karas Galina Karas Svitlana Chaika Tetiana Kucherenko Ganna Minina Marina Timchenko Oleg Melnykov Yurii Minin Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection Acta Medica Lituanica auricular cartilage stem cells regeneration inflammation intercellular matrix |
| title | Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection |
| title_full | Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection |
| title_fullStr | Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection |
| title_full_unstemmed | Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection |
| title_short | Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection |
| title_sort | regeneration of rabbit auricular cartilage after the intravenous stem cell injection |
| topic | auricular cartilage stem cells regeneration inflammation intercellular matrix |
| url | https://www.journals.vu.lt/AML/article/view/32971 |
| work_keys_str_mv | AT pavlovirych regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT nadiiashuvalova regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT antonkaras regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT galinakaras regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT svitlanachaika regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT tetianakucherenko regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT gannaminina regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT marinatimchenko regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT olegmelnykov regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection AT yuriiminin regenerationofrabbitauricularcartilageaftertheintravenousstemcellinjection |