Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening

Hi-Plex2: a simple and robust approach to targeted sequencing-based genetic screening

We have previously reported Hi-Plex, a multiplex PCR methodology for building targeted DNA sequencing libraries that offers a low-cost protocol compatible with high-throughput processing. Here, we detail an improved protocol, Hi-Plex2, that more effectively enables the robust construction of small-t...

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Bibliographic Details
Main Authors: Fleur Hammet, Khalid Mahmood, Thomas R Green, Tu Nguyen-Dumont, Melissa C Southey, Daniel D Buchanan, Andrew Lonie, Katherine L Nathanson, Fergus J Couch, Bernard J Pope, Daniel J Park
Format: Article
Language:English
Published: Taylor & Francis Group 2019-09-01
Series:BioTechniques
Subjects:
genetic screening
Hi-Plex
multiplex PCR
targeted DNA sequencing
variant detection
Online Access:https://www.future-science.com/doi/10.2144/btn-2019-0026
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