Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province
African Swine Fever (ASF) is a disease affecting pigs, caused by a double-stranded DNA virus that is not transmissible to humans or other animals. It leads to significant economic losses due to high morbidity and mortality rates, particularly in densely populated pig regions like Bali Province. Duri...
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| Format: | Article |
| Language: | Indonesian |
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Universitas Gadjah Mada
2024-12-01
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| Series: | Jurnal Sain Veteriner |
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| Online Access: | https://jurnal.ugm.ac.id/jsv/article/view/98994 |
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| author | Monica Septiani Vetnizah Juniantito Bambang Pontjo Priosoeryanto |
| author_facet | Monica Septiani Vetnizah Juniantito Bambang Pontjo Priosoeryanto |
| author_sort | Monica Septiani |
| collection | DOAJ |
| description | African Swine Fever (ASF) is a disease affecting pigs, caused by a double-stranded DNA virus that is not transmissible to humans or other animals. It leads to significant economic losses due to high morbidity and mortality rates, particularly in densely populated pig regions like Bali Province. During the ASF outbreak from June to December 2023, this study conducted histopathological research on clinical samples. Biological materials from twelve pigs confirmed positive for ASF via qPCR examination were histopathologically analyzed. Tissue samples from various organs underwent processing and examination using hematoxylin and eosin staining. Immunohistochemistry (IHC) was employed to detect the ASF virus’s p54 protein. Clinical symptoms, anatomical pathology, and histological examination revealed characteristic acute ASF lesions. Immunohistochemistry consistently showed p54 viral antigen distribution in mononuclear cells/macrophages across various organs, with the spleen and lymph nodes being dominant sites in 12 pigs (100%). This comprehensive study demonstrates the effectiveness of IHC in detecting the ASF virus and characterizing its histopathology.
Keywords: ASF; histopathology; immunohistochemistry |
| format | Article |
| id | doaj-art-a1db4b6776a0468e8c3a57fcf9895275 |
| institution | Kabale University |
| issn | 2407-3733 |
| language | Indonesian |
| publishDate | 2024-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Sain Veteriner |
| spelling | doaj-art-a1db4b6776a0468e8c3a57fcf98952752025-01-20T02:12:25ZindUniversitas Gadjah MadaJurnal Sain Veteriner2407-37332024-12-0142340040810.22146/jsv.9899436817Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali ProvinceMonica Septiani0Vetnizah Juniantito1Bambang Pontjo Priosoeryanto2IPB University/Balai Besar Veteriner DenpasarIPB UniversityIPB UniversityAfrican Swine Fever (ASF) is a disease affecting pigs, caused by a double-stranded DNA virus that is not transmissible to humans or other animals. It leads to significant economic losses due to high morbidity and mortality rates, particularly in densely populated pig regions like Bali Province. During the ASF outbreak from June to December 2023, this study conducted histopathological research on clinical samples. Biological materials from twelve pigs confirmed positive for ASF via qPCR examination were histopathologically analyzed. Tissue samples from various organs underwent processing and examination using hematoxylin and eosin staining. Immunohistochemistry (IHC) was employed to detect the ASF virus’s p54 protein. Clinical symptoms, anatomical pathology, and histological examination revealed characteristic acute ASF lesions. Immunohistochemistry consistently showed p54 viral antigen distribution in mononuclear cells/macrophages across various organs, with the spleen and lymph nodes being dominant sites in 12 pigs (100%). This comprehensive study demonstrates the effectiveness of IHC in detecting the ASF virus and characterizing its histopathology. Keywords: ASF; histopathology; immunohistochemistryhttps://jurnal.ugm.ac.id/jsv/article/view/98994asfhistopathologyimmunohistochemistry |
| spellingShingle | Monica Septiani Vetnizah Juniantito Bambang Pontjo Priosoeryanto Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province Jurnal Sain Veteriner asf histopathology immunohistochemistry |
| title | Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province |
| title_full | Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province |
| title_fullStr | Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province |
| title_full_unstemmed | Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province |
| title_short | Histopathological and Immunohistochemical Evaluation of African Swine Fever Pigs in Bali Province |
| title_sort | histopathological and immunohistochemical evaluation of african swine fever pigs in bali province |
| topic | asf histopathology immunohistochemistry |
| url | https://jurnal.ugm.ac.id/jsv/article/view/98994 |
| work_keys_str_mv | AT monicaseptiani histopathologicalandimmunohistochemicalevaluationofafricanswinefeverpigsinbaliprovince AT vetnizahjuniantito histopathologicalandimmunohistochemicalevaluationofafricanswinefeverpigsinbaliprovince AT bambangpontjopriosoeryanto histopathologicalandimmunohistochemicalevaluationofafricanswinefeverpigsinbaliprovince |