Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics
Perfluorodecanoic acid (PFDA) is a typical perfluoroalkyl substances frequently encountered in populations, posing significant risks to human health. However, research on the effects of PFDA exposure on organism metabolism and related pathogenic mechanisms is severely lacking. In this study, serum a...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015513 |
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| author | Lvyun Sun Shuling He Jiali Chen Amei Su Qiuyao Mao Wenyuan Zhang Ying Pan Jiaqian Hu Disheng Feng Yang Ouyang |
| author_facet | Lvyun Sun Shuling He Jiali Chen Amei Su Qiuyao Mao Wenyuan Zhang Ying Pan Jiaqian Hu Disheng Feng Yang Ouyang |
| author_sort | Lvyun Sun |
| collection | DOAJ |
| description | Perfluorodecanoic acid (PFDA) is a typical perfluoroalkyl substances frequently encountered in populations, posing significant risks to human health. However, research on the effects of PFDA exposure on organism metabolism and related pathogenic mechanisms is severely lacking. In this study, serum and liver samples of C57BL/6 J mice exposed to different doses of PFDA were analyzed by UPLC-HRMS-based metabolomics and lipidomics techniques. Both 1 mg/kg and 10 mg/kg PFDA exposure induced liver damage, while only 10 mg/kg PFDA exposure caused weight loss. Metabolomics analysis revealed that 330 and 515 metabolites were significantly altered in the serum and liver of mice after PFDA exposure, respectively. Most amino acids and peptides increased in the serum but decreased in the liver. Lipidomics analysis indicated that 281 and 408 lipids experienced significant alterations in the serum and liver after PFDA exposure, respectively. Most lipids, particularly multiple triacylglycerols, were downregulated in a dose-dependent manner in both serum and liver. Taken together, PFDA can induce changes in the amino acid metabolism pathway, disrupt fatty acid β-oxidation, and down-regulate glycolipid pathways in mice, resulting in disturbances in energy metabolism. These findings suggested that the liver is a critical target organ for PFDA exposure, and will also help inform future risk assessment. |
| format | Article |
| id | doaj-art-a1be178fbe0841b68ceb46e3e4677930 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-a1be178fbe0841b68ceb46e3e46779302025-01-23T05:25:42ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117475Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomicsLvyun Sun0Shuling He1Jiali Chen2Amei Su3Qiuyao Mao4Wenyuan Zhang5Ying Pan6Jiaqian Hu7Disheng Feng8Yang Ouyang9Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaDepartment of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, ChinaCollege of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; Corresponding authors.Department of Health Inspection and Quarantine, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, China; Corresponding authors.Perfluorodecanoic acid (PFDA) is a typical perfluoroalkyl substances frequently encountered in populations, posing significant risks to human health. However, research on the effects of PFDA exposure on organism metabolism and related pathogenic mechanisms is severely lacking. In this study, serum and liver samples of C57BL/6 J mice exposed to different doses of PFDA were analyzed by UPLC-HRMS-based metabolomics and lipidomics techniques. Both 1 mg/kg and 10 mg/kg PFDA exposure induced liver damage, while only 10 mg/kg PFDA exposure caused weight loss. Metabolomics analysis revealed that 330 and 515 metabolites were significantly altered in the serum and liver of mice after PFDA exposure, respectively. Most amino acids and peptides increased in the serum but decreased in the liver. Lipidomics analysis indicated that 281 and 408 lipids experienced significant alterations in the serum and liver after PFDA exposure, respectively. Most lipids, particularly multiple triacylglycerols, were downregulated in a dose-dependent manner in both serum and liver. Taken together, PFDA can induce changes in the amino acid metabolism pathway, disrupt fatty acid β-oxidation, and down-regulate glycolipid pathways in mice, resulting in disturbances in energy metabolism. These findings suggested that the liver is a critical target organ for PFDA exposure, and will also help inform future risk assessment.http://www.sciencedirect.com/science/article/pii/S0147651324015513PFDAExposureLC-MSMetabolomicsLipidomics |
| spellingShingle | Lvyun Sun Shuling He Jiali Chen Amei Su Qiuyao Mao Wenyuan Zhang Ying Pan Jiaqian Hu Disheng Feng Yang Ouyang Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics Ecotoxicology and Environmental Safety PFDA Exposure LC-MS Metabolomics Lipidomics |
| title | Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| title_full | Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| title_fullStr | Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| title_full_unstemmed | Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| title_short | Hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| title_sort | hepatic injury and metabolic perturbations in mice exposed to perfluorodecanoic acid revealed by metabolomics and lipidomics |
| topic | PFDA Exposure LC-MS Metabolomics Lipidomics |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015513 |
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