Nuclear talin-1 provides a bridge between cell adhesion and gene expression
Summary: Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we...
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| Language: | English |
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225000045 |
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| author | Alejandro J. Da Silva Hendrik S.E. Hästbacka Mikael C. Puustinen Jenny C. Pessa Jens C. Luoto Erika Sundström Benjamin T. Goult Guillaume Jacquemet Eva Henriksson Lea Sistonen |
| author_facet | Alejandro J. Da Silva Hendrik S.E. Hästbacka Mikael C. Puustinen Jenny C. Pessa Jens C. Luoto Erika Sundström Benjamin T. Goult Guillaume Jacquemet Eva Henriksson Lea Sistonen |
| author_sort | Alejandro J. Da Silva |
| collection | DOAJ |
| description | Summary: Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. To determine the functional impact of nuclear TLN1, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our findings revealed that the accumulation of nuclear TLN1 alters the expression of a subset of genes and impairs the formation of cell-cell clusters. This study introduces an additional perspective on the canonical view of TLN1 subcellular localization and function. |
| format | Article |
| id | doaj-art-a1bdd0baadce49729c7c8b9a72b274be |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-a1bdd0baadce49729c7c8b9a72b274be2025-01-20T04:17:48ZengElsevieriScience2589-00422025-02-01282111745Nuclear talin-1 provides a bridge between cell adhesion and gene expressionAlejandro J. Da Silva0Hendrik S.E. Hästbacka1Mikael C. Puustinen2Jenny C. Pessa3Jens C. Luoto4Erika Sundström5Benjamin T. Goult6Guillaume Jacquemet7Eva Henriksson8Lea Sistonen9Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, FinlandDepartment of Biochemistry, Cell & Systems Biology, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UKFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland; InFLAMES Research Flagship Center, University of Turku and Åbo Akademi University, 20520 Turku, Finland; Turku Bioimaging, University of Turku and Åbo Akademi University, 20520 Turku, FinlandFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland; Corresponding authorFaculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland; Corresponding authorSummary: Talin-1 (TLN1) is best known to activate integrin receptors and transmit mechanical stimuli to the actin cytoskeleton at focal adhesions. However, the localization of TLN1 is not restricted to focal adhesions. By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. To determine the functional impact of nuclear TLN1, we expressed a TLN1 fusion protein containing a nuclear localization signal. Our findings revealed that the accumulation of nuclear TLN1 alters the expression of a subset of genes and impairs the formation of cell-cell clusters. This study introduces an additional perspective on the canonical view of TLN1 subcellular localization and function.http://www.sciencedirect.com/science/article/pii/S2589004225000045properties of biomoleculescellular physiologymolecular biologymolecular interactioncell biology |
| spellingShingle | Alejandro J. Da Silva Hendrik S.E. Hästbacka Mikael C. Puustinen Jenny C. Pessa Jens C. Luoto Erika Sundström Benjamin T. Goult Guillaume Jacquemet Eva Henriksson Lea Sistonen Nuclear talin-1 provides a bridge between cell adhesion and gene expression iScience properties of biomolecules cellular physiology molecular biology molecular interaction cell biology |
| title | Nuclear talin-1 provides a bridge between cell adhesion and gene expression |
| title_full | Nuclear talin-1 provides a bridge between cell adhesion and gene expression |
| title_fullStr | Nuclear talin-1 provides a bridge between cell adhesion and gene expression |
| title_full_unstemmed | Nuclear talin-1 provides a bridge between cell adhesion and gene expression |
| title_short | Nuclear talin-1 provides a bridge between cell adhesion and gene expression |
| title_sort | nuclear talin 1 provides a bridge between cell adhesion and gene expression |
| topic | properties of biomolecules cellular physiology molecular biology molecular interaction cell biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225000045 |
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