Optimization of RHDV type 1 and 2 inactivation modes
The purpose of these studies was to optimize RHDV type 1 and 2 (RHDV1 and RHDV2) inactivation modes to use the obtained antigens in inactivated vaccines and diagnosticums. The inactivating effect of aminoethylethylenimine and β-propiolactone was studied in different concentrations in correlation wit...
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Da Vinci Media
2021-03-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/539 |
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| author | E. D. Kunikova N. V. Moroz M. A. Dolgova L. V. Malakhova I. A. Komarov |
| author_facet | E. D. Kunikova N. V. Moroz M. A. Dolgova L. V. Malakhova I. A. Komarov |
| author_sort | E. D. Kunikova |
| collection | DOAJ |
| description | The purpose of these studies was to optimize RHDV type 1 and 2 (RHDV1 and RHDV2) inactivation modes to use the obtained antigens in inactivated vaccines and diagnosticums. The inactivating effect of aminoethylethylenimine and β-propiolactone was studied in different concentrations in correlation with the exposure time and temperature. The correlation between the inactivating effect of the compound used and the accepted test conditions (concentration, temperature, and exposure time) was studied on a group of rabbits, each of which was injected intramuscularly with 1 cm3 of the inactivated material sample. At the end of the maximum exposure interval, a control sample of the viral material, kept under the same conditions without any inactivant added was similarly tested. Lethality was considered to evaluate the damaging action in the test and control groups: L = m/n, where m is the number of dead animals; n is the total number of rabbits in the group for testing of the inactivated material sample. The postmortem diagnosis was confirmed by testing the rabbit liver tissue homogenate for relative antigens using ELISA. It was found that aminoethylethylenimine and β-propiolactone did not have the same effect on the studied variants of the virus. In order to preserve at maximum the antigenic structures of the virus, the following inactivation modes were considered to be optimal: for RHDV1-aminoethylethylenimine at a concentration of 0.3% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration of 0.1–0.3% at 25–37 °С, exposure time – 24–48 hours; for RHDV2 – aminoethylethylenimine at a concentration of 1% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration 0.3% at 25 °С, exposure time – 24 hours. |
| format | Article |
| id | doaj-art-a1b4a122541d453db6dd47500a43c1a1 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2021-03-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-a1b4a122541d453db6dd47500a43c1a12025-02-06T09:52:08ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592021-03-0111222810.29326/2304-196X-2021-1-36-22-28493Optimization of RHDV type 1 and 2 inactivation modesE. D. Kunikova0N. V. Moroz1M. A. Dolgova2L. V. Malakhova3I. A. Komarov4FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FSBEI HE “Kostroma State Agricultural Academy” (FSBEI HE Kostroma SAA)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)The purpose of these studies was to optimize RHDV type 1 and 2 (RHDV1 and RHDV2) inactivation modes to use the obtained antigens in inactivated vaccines and diagnosticums. The inactivating effect of aminoethylethylenimine and β-propiolactone was studied in different concentrations in correlation with the exposure time and temperature. The correlation between the inactivating effect of the compound used and the accepted test conditions (concentration, temperature, and exposure time) was studied on a group of rabbits, each of which was injected intramuscularly with 1 cm3 of the inactivated material sample. At the end of the maximum exposure interval, a control sample of the viral material, kept under the same conditions without any inactivant added was similarly tested. Lethality was considered to evaluate the damaging action in the test and control groups: L = m/n, where m is the number of dead animals; n is the total number of rabbits in the group for testing of the inactivated material sample. The postmortem diagnosis was confirmed by testing the rabbit liver tissue homogenate for relative antigens using ELISA. It was found that aminoethylethylenimine and β-propiolactone did not have the same effect on the studied variants of the virus. In order to preserve at maximum the antigenic structures of the virus, the following inactivation modes were considered to be optimal: for RHDV1-aminoethylethylenimine at a concentration of 0.3% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration of 0.1–0.3% at 25–37 °С, exposure time – 24–48 hours; for RHDV2 – aminoethylethylenimine at a concentration of 1% at 37 °C, exposure time – 72 hours, or β-propiolactone at a concentration 0.3% at 25 °С, exposure time – 24 hours.https://veterinary.arriah.ru/jour/article/view/539viral hemorrhagic disease of rabbitsinactivated vaccineaminoethylethylenimineβ-propiolactoneinactivation of rabbit hemorrhagic disease virus |
| spellingShingle | E. D. Kunikova N. V. Moroz M. A. Dolgova L. V. Malakhova I. A. Komarov Optimization of RHDV type 1 and 2 inactivation modes Ветеринария сегодня viral hemorrhagic disease of rabbits inactivated vaccine aminoethylethylenimine β-propiolactone inactivation of rabbit hemorrhagic disease virus |
| title | Optimization of RHDV type 1 and 2 inactivation modes |
| title_full | Optimization of RHDV type 1 and 2 inactivation modes |
| title_fullStr | Optimization of RHDV type 1 and 2 inactivation modes |
| title_full_unstemmed | Optimization of RHDV type 1 and 2 inactivation modes |
| title_short | Optimization of RHDV type 1 and 2 inactivation modes |
| title_sort | optimization of rhdv type 1 and 2 inactivation modes |
| topic | viral hemorrhagic disease of rabbits inactivated vaccine aminoethylethylenimine β-propiolactone inactivation of rabbit hemorrhagic disease virus |
| url | https://veterinary.arriah.ru/jour/article/view/539 |
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