Multipyrene Tandem Probes for Point Mutations Detection in DNA
Here we report design, synthesis and characterization of highly sensitive, specific and stable in biological systems fluorescent probes for point mutation detection in DNA. The tandems of 3′- and 5′-mono- and bis-pyrene conjugated oligo(2′-O-methylribonucleotides), protected by 3′-“inverted” thymidi...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2013/860457 |
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| author | Svetlana A. Kholodar Darya S. Novopashina Mariya I. Meschaninova Alya G. Venyaminova |
| author_facet | Svetlana A. Kholodar Darya S. Novopashina Mariya I. Meschaninova Alya G. Venyaminova |
| author_sort | Svetlana A. Kholodar |
| collection | DOAJ |
| description | Here we report design, synthesis and characterization of highly sensitive, specific and stable in biological systems fluorescent probes for point mutation detection in DNA. The tandems of 3′- and 5′-mono- and bis-pyrene conjugated oligo(2′-O-methylribonucleotides), protected by 3′-“inverted” thymidine, were constructed and their potential as new instruments for genetic diagnostics was studied. Novel probes have been shown to exhibit an ability to form stable duplexes with DNA target due to the stabilizing effect of multiple pyrene units at the junction. The relationship between fluorescent properties of developed probes, the number of pyrene residues at the tandem junction, and the location of point mutation has been studied. On the basis of the data obtained, we have chosen the probes possessing the highest fluorescence intensity along with the best mismatch discrimination and deletion and insertion detection ability. Application of developed probes for detection of polymorphism C677T in MTHFR gene has been demonstrated on model systems. |
| format | Article |
| id | doaj-art-a18237715add4d65a7b8a72802c83bc9 |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-a18237715add4d65a7b8a72802c83bc92025-02-03T01:11:39ZengWileyJournal of Nucleic Acids2090-02012090-021X2013-01-01201310.1155/2013/860457860457Multipyrene Tandem Probes for Point Mutations Detection in DNASvetlana A. Kholodar0Darya S. Novopashina1Mariya I. Meschaninova2Alya G. Venyaminova3Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, RussiaHere we report design, synthesis and characterization of highly sensitive, specific and stable in biological systems fluorescent probes for point mutation detection in DNA. The tandems of 3′- and 5′-mono- and bis-pyrene conjugated oligo(2′-O-methylribonucleotides), protected by 3′-“inverted” thymidine, were constructed and their potential as new instruments for genetic diagnostics was studied. Novel probes have been shown to exhibit an ability to form stable duplexes with DNA target due to the stabilizing effect of multiple pyrene units at the junction. The relationship between fluorescent properties of developed probes, the number of pyrene residues at the tandem junction, and the location of point mutation has been studied. On the basis of the data obtained, we have chosen the probes possessing the highest fluorescence intensity along with the best mismatch discrimination and deletion and insertion detection ability. Application of developed probes for detection of polymorphism C677T in MTHFR gene has been demonstrated on model systems.http://dx.doi.org/10.1155/2013/860457 |
| spellingShingle | Svetlana A. Kholodar Darya S. Novopashina Mariya I. Meschaninova Alya G. Venyaminova Multipyrene Tandem Probes for Point Mutations Detection in DNA Journal of Nucleic Acids |
| title | Multipyrene Tandem Probes for Point Mutations Detection in DNA |
| title_full | Multipyrene Tandem Probes for Point Mutations Detection in DNA |
| title_fullStr | Multipyrene Tandem Probes for Point Mutations Detection in DNA |
| title_full_unstemmed | Multipyrene Tandem Probes for Point Mutations Detection in DNA |
| title_short | Multipyrene Tandem Probes for Point Mutations Detection in DNA |
| title_sort | multipyrene tandem probes for point mutations detection in dna |
| url | http://dx.doi.org/10.1155/2013/860457 |
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