Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization

Madhuca longifolia (mahua), a deciduous tree growing throughout the subtropical region of the India is a potential source of differentiated essential oils (EOs) with distinct bioactives. The study aimed to assess the effect of extraction methods on the quantitative and qualitative divergence in the...

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Main Authors: Achala Gupta, Mohammed A. Bareen, Priyanka Prasad, Anita Srivastava, Jatindra K. Sahu, Hariprasad P
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Food Chemistry Advances
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772753X24002272
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author Achala Gupta
Mohammed A. Bareen
Priyanka Prasad
Anita Srivastava
Jatindra K. Sahu
Hariprasad P
author_facet Achala Gupta
Mohammed A. Bareen
Priyanka Prasad
Anita Srivastava
Jatindra K. Sahu
Hariprasad P
author_sort Achala Gupta
collection DOAJ
description Madhuca longifolia (mahua), a deciduous tree growing throughout the subtropical region of the India is a potential source of differentiated essential oils (EOs) with distinct bioactives. The study aimed to assess the effect of extraction methods on the quantitative and qualitative divergence in the chemical composition of flavor compounds of mahua flower EO. The advanced supercritical CO2 extraction (SCFE) and ultrasound-assisted solvent extraction (UAE) was compared to the conventional extraction techniques such as hydrodistillation (HD), enzyme-assisted hydrodistillation (HDE) and soxhlet (SOX) for their high-quality essential oil (EO) extraction efficiency. The maximum EO yield of 2.074% was obtained by SCFE followed by HD (1.64%). The presence of significant amount of terpenoids, including 2-acetyl-1-pyrroline, γ-terpinene, myrcene, α-terpineol, limonene, and L-linalool in EO was noticed with chemical profiling using GC-MS and E-Nose. SCFE method yielded EOs with best terpenoids as compared to other extraction methods. Furthermore, EO extracted using SCFE had higher total phenol and flavonoid content of 92.56mg GAE/g, 62.18mg QE/g respectively, and DPPH (100.06µg/mL), FRAP (91.52µM Fe(II)/g) antioxidant activity as compared to other extraction methods. A significantly (p<0.05) higher α-glucosidase and α-amylase inhibition of 37.16% and 43%, respectively, was shown by SCFE EOs. Total carotenoid content of EOs produced using all the extraction techniques ranged from 9.94 (HDE) to 29.673 (SCFE) mg/100g β-carotene. The “van krevelen” plots of CHNS analysis revealed ‘core metabolites’. This study illustrates that SCFE is a more effective method for EO extraction of high quality from low fat plant materials such as mahua flowers as compared to HD, HDE, SOX, and UAE.
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publisher Elsevier
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spelling doaj-art-a1712f61d4a24f20a4300c78e3af2a1d2025-08-20T01:59:34ZengElsevierFood Chemistry Advances2772-753X2024-12-01510083210.1016/j.focha.2024.100832Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterizationAchala Gupta0Mohammed A. Bareen1Priyanka Prasad2Anita Srivastava3Jatindra K. Sahu4Hariprasad P5Food Customization and Research Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, IndiaFood Customization and Research Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, IndiaFood Customization and Research Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, IndiaFood Customization and Research Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, IndiaFood Customization and Research Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi 110 016, India; Corresponding author.Environmental Biotechnology Lab, Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, IndiaMadhuca longifolia (mahua), a deciduous tree growing throughout the subtropical region of the India is a potential source of differentiated essential oils (EOs) with distinct bioactives. The study aimed to assess the effect of extraction methods on the quantitative and qualitative divergence in the chemical composition of flavor compounds of mahua flower EO. The advanced supercritical CO2 extraction (SCFE) and ultrasound-assisted solvent extraction (UAE) was compared to the conventional extraction techniques such as hydrodistillation (HD), enzyme-assisted hydrodistillation (HDE) and soxhlet (SOX) for their high-quality essential oil (EO) extraction efficiency. The maximum EO yield of 2.074% was obtained by SCFE followed by HD (1.64%). The presence of significant amount of terpenoids, including 2-acetyl-1-pyrroline, γ-terpinene, myrcene, α-terpineol, limonene, and L-linalool in EO was noticed with chemical profiling using GC-MS and E-Nose. SCFE method yielded EOs with best terpenoids as compared to other extraction methods. Furthermore, EO extracted using SCFE had higher total phenol and flavonoid content of 92.56mg GAE/g, 62.18mg QE/g respectively, and DPPH (100.06µg/mL), FRAP (91.52µM Fe(II)/g) antioxidant activity as compared to other extraction methods. A significantly (p<0.05) higher α-glucosidase and α-amylase inhibition of 37.16% and 43%, respectively, was shown by SCFE EOs. Total carotenoid content of EOs produced using all the extraction techniques ranged from 9.94 (HDE) to 29.673 (SCFE) mg/100g β-carotene. The “van krevelen” plots of CHNS analysis revealed ‘core metabolites’. This study illustrates that SCFE is a more effective method for EO extraction of high quality from low fat plant materials such as mahua flowers as compared to HD, HDE, SOX, and UAE.http://www.sciencedirect.com/science/article/pii/S2772753X24002272Mahua flowersEssential oilHydro-distillationUltrasound-assisted extractionSupercritical CO2 extractionE-nose
spellingShingle Achala Gupta
Mohammed A. Bareen
Priyanka Prasad
Anita Srivastava
Jatindra K. Sahu
Hariprasad P
Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
Food Chemistry Advances
Mahua flowers
Essential oil
Hydro-distillation
Ultrasound-assisted extraction
Supercritical CO2 extraction
E-nose
title Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
title_full Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
title_fullStr Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
title_full_unstemmed Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
title_short Supercritical carbon dioxide extraction of essential oils from Madhuca Longifolia flowers and its characterization
title_sort supercritical carbon dioxide extraction of essential oils from madhuca longifolia flowers and its characterization
topic Mahua flowers
Essential oil
Hydro-distillation
Ultrasound-assisted extraction
Supercritical CO2 extraction
E-nose
url http://www.sciencedirect.com/science/article/pii/S2772753X24002272
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