Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization
Sterilization is essential in preventing the spread of pathogenic bacteria and antibiotic resistance in hospital settings. This study examines the microbial growth and antibiotic resistance of seven medical instruments from the Central Supply Sterile Department, before and after sterilization. The i...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2025-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/03/bioconf_ichbs2025_01029.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539702470639616 |
|---|---|
| author | Hapsari Woro Sajidan Suranto Hikmayani Nur Hafidha |
| author_facet | Hapsari Woro Sajidan Suranto Hikmayani Nur Hafidha |
| author_sort | Hapsari Woro |
| collection | DOAJ |
| description | Sterilization is essential in preventing the spread of pathogenic bacteria and antibiotic resistance in hospital settings. This study examines the microbial growth and antibiotic resistance of seven medical instruments from the Central Supply Sterile Department, before and after sterilization. The instruments analyzed include arterial clamps, anatomical forceps, tissue scissors, surgical forceps, nal puder, open small tray, and instrument tray. The methodology involved in vitro microbiological testing, including Gram staining, colony counting, and the Vitek 2 system for bacterial identification and antibiotic susceptibility testing (AST). Results showed that before sterilization, nal puder was contaminated with Staphylococcus hominis ssp hominis (Gram-positive) and Bacillus sp. (Gram-positive), while the instrument tray was contaminated with Pseudomonas stutzeri (Gramnegative). After sterilization and a 90-day storage period, Staphylococcus hominis ssp hominis was detected on the open small tray. AST revealed an increase in bacterial sensitivity to antibiotics, from 12 to 14 types poststerilization. The minimum inhibitory concentration (MIC) for Benzylpenicillin decreased from 0.25 μg/mL (resistant) to ≤0.03 μg/mL (sensitive). This study highlights the critical role of sterilization in infection control and antibiotic resistance management in healthcare settings. |
| format | Article |
| id | doaj-art-a1630eca939444d48add803b4c47052f |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-a1630eca939444d48add803b4c47052f2025-02-05T10:42:50ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011520102910.1051/bioconf/202515201029bioconf_ichbs2025_01029Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilizationHapsari Woro0Sajidan1Suranto2Hikmayani Nur Hafidha3Doctoral Program of Environmental Science, Post Graduate School of Universitas Sebelas MaretDepartement of Biology Education, Faculty of teacher and education Universitas Sebelas MaretDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas MaretFaculty of Medicine Universitas Sebelas MaretSterilization is essential in preventing the spread of pathogenic bacteria and antibiotic resistance in hospital settings. This study examines the microbial growth and antibiotic resistance of seven medical instruments from the Central Supply Sterile Department, before and after sterilization. The instruments analyzed include arterial clamps, anatomical forceps, tissue scissors, surgical forceps, nal puder, open small tray, and instrument tray. The methodology involved in vitro microbiological testing, including Gram staining, colony counting, and the Vitek 2 system for bacterial identification and antibiotic susceptibility testing (AST). Results showed that before sterilization, nal puder was contaminated with Staphylococcus hominis ssp hominis (Gram-positive) and Bacillus sp. (Gram-positive), while the instrument tray was contaminated with Pseudomonas stutzeri (Gramnegative). After sterilization and a 90-day storage period, Staphylococcus hominis ssp hominis was detected on the open small tray. AST revealed an increase in bacterial sensitivity to antibiotics, from 12 to 14 types poststerilization. The minimum inhibitory concentration (MIC) for Benzylpenicillin decreased from 0.25 μg/mL (resistant) to ≤0.03 μg/mL (sensitive). This study highlights the critical role of sterilization in infection control and antibiotic resistance management in healthcare settings.https://www.bio-conferences.org/articles/bioconf/pdf/2025/03/bioconf_ichbs2025_01029.pdf |
| spellingShingle | Hapsari Woro Sajidan Suranto Hikmayani Nur Hafidha Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization BIO Web of Conferences |
| title | Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization |
| title_full | Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization |
| title_fullStr | Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization |
| title_full_unstemmed | Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization |
| title_short | Analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department (CSSD) of hospitals before and after sterilization |
| title_sort | analysis of microorganisms and antibiotic resistance in medical instruments in the central sterile supply department cssd of hospitals before and after sterilization |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/03/bioconf_ichbs2025_01029.pdf |
| work_keys_str_mv | AT hapsariworo analysisofmicroorganismsandantibioticresistanceinmedicalinstrumentsinthecentralsterilesupplydepartmentcssdofhospitalsbeforeandaftersterilization AT sajidan analysisofmicroorganismsandantibioticresistanceinmedicalinstrumentsinthecentralsterilesupplydepartmentcssdofhospitalsbeforeandaftersterilization AT suranto analysisofmicroorganismsandantibioticresistanceinmedicalinstrumentsinthecentralsterilesupplydepartmentcssdofhospitalsbeforeandaftersterilization AT hikmayaninurhafidha analysisofmicroorganismsandantibioticresistanceinmedicalinstrumentsinthecentralsterilesupplydepartmentcssdofhospitalsbeforeandaftersterilization |