Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation
Hair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are of great interest due to their safety. In this study, we utilize reverse network pharmacology to screen for key targets of the Wnt/β-catenin signaling pathway and the TGFβ/BMP signaling pa...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/1/68 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588787267403776 |
|---|---|
| author | Jiajia Xu Congfen He Rui Tian |
| author_facet | Jiajia Xu Congfen He Rui Tian |
| author_sort | Jiajia Xu |
| collection | DOAJ |
| description | Hair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are of great interest due to their safety. In this study, we utilize reverse network pharmacology to screen for key targets of the Wnt/β-catenin signaling pathway and the TGFβ/BMP signaling pathway, as well as key differential lipids, for plant raw materials selection. The aim is to identify plant raw materials that may have anti-hair loss properties and to validate these findings through cell experiments. Licorice, salvia miltiorrhiza, mulberry leaf, ephedra and curcumae radix were found that may possess anti-hair loss effects. Licorice water extract (LWE), salvia miltiorrhiza water extract (SMWE), mulberry leaf water extract (MLWE), ephedra water extract (EWE) and curcumae radix water extract (CRWE) did not exhibit cytotoxicity on human dermal papilla cells (HDPCs). Through ALP staining, it was found that the expression of ALP in HDPCs treated with LWE, SMWE, MLWE, EWE and CRWE was enhanced. In addition, LWE, SMWE, MLWE, EWE and CRWE have reduced the expression of hair growth inhibitory factor TGF-β1 and inflammatory factor IL-6. Additionally, various water extracts can enhance the secretion of VEGF, with high concentrations of SMWE, EWE and CRWE exhibiting better efficacy. Furthermore, β-catenin, a key factor of the Wnt/β-catenin signaling pathway, was enhanced by LWE, SMWE, MLWE, EWE and CRWE treatment in cultured HDPCs. In conclusion, all five plant raw materials showed some anti-hair loss potential, providing theoretical support for their application in anti-hair loss products. |
| format | Article |
| id | doaj-art-a13b83f314254ad6b5c6fec96d7c64a9 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-a13b83f314254ad6b5c6fec96d7c64a92025-01-24T13:27:38ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-01-014716810.3390/cimb47010068Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental ValidationJiajia Xu0Congfen He1Rui Tian2Beijing Key Laboratory of Plant Resources Research and Development, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resources Research and Development, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Key Laboratory of Plant Resources Research and Development, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, ChinaHair loss is one of the skin conditions that can affect people’s mental health. Plant raw material extracts are of great interest due to their safety. In this study, we utilize reverse network pharmacology to screen for key targets of the Wnt/β-catenin signaling pathway and the TGFβ/BMP signaling pathway, as well as key differential lipids, for plant raw materials selection. The aim is to identify plant raw materials that may have anti-hair loss properties and to validate these findings through cell experiments. Licorice, salvia miltiorrhiza, mulberry leaf, ephedra and curcumae radix were found that may possess anti-hair loss effects. Licorice water extract (LWE), salvia miltiorrhiza water extract (SMWE), mulberry leaf water extract (MLWE), ephedra water extract (EWE) and curcumae radix water extract (CRWE) did not exhibit cytotoxicity on human dermal papilla cells (HDPCs). Through ALP staining, it was found that the expression of ALP in HDPCs treated with LWE, SMWE, MLWE, EWE and CRWE was enhanced. In addition, LWE, SMWE, MLWE, EWE and CRWE have reduced the expression of hair growth inhibitory factor TGF-β1 and inflammatory factor IL-6. Additionally, various water extracts can enhance the secretion of VEGF, with high concentrations of SMWE, EWE and CRWE exhibiting better efficacy. Furthermore, β-catenin, a key factor of the Wnt/β-catenin signaling pathway, was enhanced by LWE, SMWE, MLWE, EWE and CRWE treatment in cultured HDPCs. In conclusion, all five plant raw materials showed some anti-hair loss potential, providing theoretical support for their application in anti-hair loss products.https://www.mdpi.com/1467-3045/47/1/68hair lossreverse network pharmacologyWnt/β-catenin signaling pathwayTGFβ/BMP signaling pathwaylipidslicorice |
| spellingShingle | Jiajia Xu Congfen He Rui Tian Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation Current Issues in Molecular Biology hair loss reverse network pharmacology Wnt/β-catenin signaling pathway TGFβ/BMP signaling pathway lipids licorice |
| title | Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation |
| title_full | Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation |
| title_fullStr | Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation |
| title_full_unstemmed | Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation |
| title_short | Screening of Anti-Hair Loss Plant Raw Materials Based on Reverse Network Pharmacology and Experimental Validation |
| title_sort | screening of anti hair loss plant raw materials based on reverse network pharmacology and experimental validation |
| topic | hair loss reverse network pharmacology Wnt/β-catenin signaling pathway TGFβ/BMP signaling pathway lipids licorice |
| url | https://www.mdpi.com/1467-3045/47/1/68 |
| work_keys_str_mv | AT jiajiaxu screeningofantihairlossplantrawmaterialsbasedonreversenetworkpharmacologyandexperimentalvalidation AT congfenhe screeningofantihairlossplantrawmaterialsbasedonreversenetworkpharmacologyandexperimentalvalidation AT ruitian screeningofantihairlossplantrawmaterialsbasedonreversenetworkpharmacologyandexperimentalvalidation |