Comparison of Biomarkers in Blood and Saliva in Healthy Adults
Researchers measure biomarkers as a reflection of patient health status or intervention outcomes. While blood is generally regarded as the best body fluid for evaluation of systemic processes, substitution of saliva samples for blood would be less invasive and more convenient. The concentration of s...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Nursing Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/246178 |
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| author | Sarah Williamson Cindy Munro Rita Pickler Mary Jo Grap R. K. Elswick |
| author_facet | Sarah Williamson Cindy Munro Rita Pickler Mary Jo Grap R. K. Elswick |
| author_sort | Sarah Williamson |
| collection | DOAJ |
| description | Researchers measure biomarkers as a reflection of patient health status or intervention outcomes. While blood is generally regarded as the best body fluid for evaluation of systemic processes, substitution of saliva samples for blood would be less invasive and more convenient. The concentration of specific biomarkers may differ between blood and saliva. The objective of this study was to compare multiple biomarkers (27 cytokines) in plasma samples, passive drool saliva samples, and filter paper saliva samples in 50 healthy adults. Demographic data and three samples were obtained from each subject: saliva collected on filter paper over 1 minute, saliva collected by passive drool over 30 seconds, and venous blood (3 mL) collected by venipuncture. Cytokines were assayed using Bio-Rad multiplex suspension array technology. Descriptive statistics and pairwise correlations were used for data analysis. The sample was 52% male and 74% white. Mean age was 26 (range = 19–63 years, sd = 9.7). The most consistent and highest correlations were between the passive drool and filter paper saliva samples, although relationships were dependent on the specific biomarker. Correlations were not robust enough to support substitution of one collection method for another. There was little correlation between the plasma and passive drool saliva samples. Caution should be used in substituting saliva for blood, and relationships differ by biomarker. |
| format | Article |
| id | doaj-art-a12567d6b48f4c02aec487b1f21ecd7b |
| institution | Kabale University |
| issn | 2090-1429 2090-1437 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Nursing Research and Practice |
| spelling | doaj-art-a12567d6b48f4c02aec487b1f21ecd7b2025-02-03T05:53:36ZengWileyNursing Research and Practice2090-14292090-14372012-01-01201210.1155/2012/246178246178Comparison of Biomarkers in Blood and Saliva in Healthy AdultsSarah Williamson0Cindy Munro1Rita Pickler2Mary Jo Grap3R. K. Elswick4Howard Hughes Medical Institute 2010 Summer Scholar, Virginia Commonwealth University, Richmond, VA 23298-0567, USAResearch and Innovation, University of South Florida College of Nursing, Tampa, FL 33612-4766, USACenter for Professional Excellence, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USADepartment of Adult Health and Nursing Systems, Virginia Commonwealth University, Richmond, VA 23298-0567, USADepartment of Adult Health and Nursing Systems, Virginia Commonwealth University, Richmond, VA 23298-0567, USAResearchers measure biomarkers as a reflection of patient health status or intervention outcomes. While blood is generally regarded as the best body fluid for evaluation of systemic processes, substitution of saliva samples for blood would be less invasive and more convenient. The concentration of specific biomarkers may differ between blood and saliva. The objective of this study was to compare multiple biomarkers (27 cytokines) in plasma samples, passive drool saliva samples, and filter paper saliva samples in 50 healthy adults. Demographic data and three samples were obtained from each subject: saliva collected on filter paper over 1 minute, saliva collected by passive drool over 30 seconds, and venous blood (3 mL) collected by venipuncture. Cytokines were assayed using Bio-Rad multiplex suspension array technology. Descriptive statistics and pairwise correlations were used for data analysis. The sample was 52% male and 74% white. Mean age was 26 (range = 19–63 years, sd = 9.7). The most consistent and highest correlations were between the passive drool and filter paper saliva samples, although relationships were dependent on the specific biomarker. Correlations were not robust enough to support substitution of one collection method for another. There was little correlation between the plasma and passive drool saliva samples. Caution should be used in substituting saliva for blood, and relationships differ by biomarker.http://dx.doi.org/10.1155/2012/246178 |
| spellingShingle | Sarah Williamson Cindy Munro Rita Pickler Mary Jo Grap R. K. Elswick Comparison of Biomarkers in Blood and Saliva in Healthy Adults Nursing Research and Practice |
| title | Comparison of Biomarkers in Blood and Saliva in Healthy Adults |
| title_full | Comparison of Biomarkers in Blood and Saliva in Healthy Adults |
| title_fullStr | Comparison of Biomarkers in Blood and Saliva in Healthy Adults |
| title_full_unstemmed | Comparison of Biomarkers in Blood and Saliva in Healthy Adults |
| title_short | Comparison of Biomarkers in Blood and Saliva in Healthy Adults |
| title_sort | comparison of biomarkers in blood and saliva in healthy adults |
| url | http://dx.doi.org/10.1155/2012/246178 |
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