Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i>
Lactobacillus is a key genus of probiotics commonly utilized for the treatment of oral infections The primary aim of our research was to investigate the probiotic potential of the newly isolated <i>Levilactobacillus brevis</i> DPL5 strain from human breast milk, focusing on its ability t...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/160 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587870200659968 |
|---|---|
| author | Ivan Iliev Galina Yahubyan Elena Apostolova-Kuzova Mariyana Gozmanova Daniela Mollova Iliya Iliev Lena Ilieva Mariana Marhova Velizar Gochev Vesselin Baev |
| author_facet | Ivan Iliev Galina Yahubyan Elena Apostolova-Kuzova Mariyana Gozmanova Daniela Mollova Iliya Iliev Lena Ilieva Mariana Marhova Velizar Gochev Vesselin Baev |
| author_sort | Ivan Iliev |
| collection | DOAJ |
| description | Lactobacillus is a key genus of probiotics commonly utilized for the treatment of oral infections The primary aim of our research was to investigate the probiotic potential of the newly isolated <i>Levilactobacillus brevis</i> DPL5 strain from human breast milk, focusing on its ability to combat biofilm-forming pathogens such as <i>Staphylococcus aureus</i>. Employing in vitro approaches, we demonstrate <i>L. brevis</i> DPL5′s ability to endure at pH 3 with survival rates above 30%, and withstand the osmotic stress often found during industrial processes like fermentation and freeze drying, retaining over 90% viability. The lyophilized cell-free supernatant of <i>L. brevis</i> DPL5 had a significant antagonistic effect against biofilm-producing nasal strains of <i>Staphylococcus aureus</i>, and it completely eradicated biofilms at subinhibitory concentrations of 20 mg·mL<sup>−1</sup>. Higher concentrations of 69 mg·mL<sup>−1</sup> were found to have a 99% bactericidal effect, based on the conducted probability analysis, indicating the production of bactericidal bioactive extracellular compounds capable of disrupting the biofilm formation of pathogens like <i>S. aureus</i>. Furthermore, genome-wide sequencing and analysis of <i>L. brevis</i> DPL5 with cutting-edge Nanopore technology has uncovered over 50 genes linked to probiotic activity, supporting its ability to adapt and thrive in the harsh gut environment. The genome also contains multiple biosynthetic gene clusters such as lanthipeptide class IV, Type III polyketide synthase (T3PKS), and ribosomally synthesized, and post-translationally modified peptides (RiPP-like compounds), all of which are associated with antibacterial properties. Our study paves the way for the further exploration of DPL5, setting the stage for innovative, nature-inspired solutions to combat stubborn bacterial infections. |
| format | Article |
| id | doaj-art-a0f7e3e3ff944e27bbdc35cc864614c3 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-a0f7e3e3ff944e27bbdc35cc864614c32025-01-24T13:42:52ZengMDPI AGMicroorganisms2076-26072025-01-0113116010.3390/microorganisms13010160Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i>Ivan Iliev0Galina Yahubyan1Elena Apostolova-Kuzova2Mariyana Gozmanova3Daniela Mollova4Iliya Iliev5Lena Ilieva6Mariana Marhova7Velizar Gochev8Vesselin Baev9Department of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Molecular Biology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Molecular Biology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Molecular Biology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Biochemistry and Microbiology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaDepartment of Molecular Biology, Faculty of Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, BulgariaLactobacillus is a key genus of probiotics commonly utilized for the treatment of oral infections The primary aim of our research was to investigate the probiotic potential of the newly isolated <i>Levilactobacillus brevis</i> DPL5 strain from human breast milk, focusing on its ability to combat biofilm-forming pathogens such as <i>Staphylococcus aureus</i>. Employing in vitro approaches, we demonstrate <i>L. brevis</i> DPL5′s ability to endure at pH 3 with survival rates above 30%, and withstand the osmotic stress often found during industrial processes like fermentation and freeze drying, retaining over 90% viability. The lyophilized cell-free supernatant of <i>L. brevis</i> DPL5 had a significant antagonistic effect against biofilm-producing nasal strains of <i>Staphylococcus aureus</i>, and it completely eradicated biofilms at subinhibitory concentrations of 20 mg·mL<sup>−1</sup>. Higher concentrations of 69 mg·mL<sup>−1</sup> were found to have a 99% bactericidal effect, based on the conducted probability analysis, indicating the production of bactericidal bioactive extracellular compounds capable of disrupting the biofilm formation of pathogens like <i>S. aureus</i>. Furthermore, genome-wide sequencing and analysis of <i>L. brevis</i> DPL5 with cutting-edge Nanopore technology has uncovered over 50 genes linked to probiotic activity, supporting its ability to adapt and thrive in the harsh gut environment. The genome also contains multiple biosynthetic gene clusters such as lanthipeptide class IV, Type III polyketide synthase (T3PKS), and ribosomally synthesized, and post-translationally modified peptides (RiPP-like compounds), all of which are associated with antibacterial properties. Our study paves the way for the further exploration of DPL5, setting the stage for innovative, nature-inspired solutions to combat stubborn bacterial infections.https://www.mdpi.com/2076-2607/13/1/160<i>Levilactobacillus brevis</i><i>Staphylococcus aureus</i>LABgenome sequencingprobiotic and anti-biofilm capacity |
| spellingShingle | Ivan Iliev Galina Yahubyan Elena Apostolova-Kuzova Mariyana Gozmanova Daniela Mollova Iliya Iliev Lena Ilieva Mariana Marhova Velizar Gochev Vesselin Baev Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> Microorganisms <i>Levilactobacillus brevis</i> <i>Staphylococcus aureus</i> LAB genome sequencing probiotic and anti-biofilm capacity |
| title | Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> |
| title_full | Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> |
| title_fullStr | Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> |
| title_short | Characterization and Probiotic Potential of <i>Levilactobacillus brevis</i> DPL5: A Novel Strain Isolated from Human Breast Milk with Antimicrobial Properties Against Biofilm-Forming <i>Staphylococcus aureus</i> |
| title_sort | characterization and probiotic potential of i levilactobacillus brevis i dpl5 a novel strain isolated from human breast milk with antimicrobial properties against biofilm forming i staphylococcus aureus i |
| topic | <i>Levilactobacillus brevis</i> <i>Staphylococcus aureus</i> LAB genome sequencing probiotic and anti-biofilm capacity |
| url | https://www.mdpi.com/2076-2607/13/1/160 |
| work_keys_str_mv | AT ivaniliev characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT galinayahubyan characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT elenaapostolovakuzova characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT mariyanagozmanova characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT danielamollova characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT iliyailiev characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT lenailieva characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT marianamarhova characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT velizargochev characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi AT vesselinbaev characterizationandprobioticpotentialofilevilactobacillusbrevisidpl5anovelstrainisolatedfromhumanbreastmilkwithantimicrobialpropertiesagainstbiofilmformingistaphylococcusaureusi |