Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants
This study is conducted to find out the effect of genetic transformation by Agrobacterium rhizogenes ATCC13332 on the anatomical features of Eruca sativa. Stem explants and leaves were inoculated by direct injection of A. rhizogenes. The results indicated the high response of E. sativa, as the perce...
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College of Agriculture
2023-12-01
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| Series: | Mesopotamia Journal of Agriculture |
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| Online Access: | https://magrj.mosuljournals.com/article_181687_fd85cb3b8c407b94cad511463384ef6d.pdf |
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| author | Raghad Abdullah Rasha Al-Jirjees Shifa Salih |
| author_facet | Raghad Abdullah Rasha Al-Jirjees Shifa Salih |
| author_sort | Raghad Abdullah |
| collection | DOAJ |
| description | This study is conducted to find out the effect of genetic transformation by Agrobacterium rhizogenes ATCC13332 on the anatomical features of Eruca sativa. Stem explants and leaves were inoculated by direct injection of A. rhizogenes. The results indicated the high response of E. sativa, as the percentage of hairy roots formation was 95.3% for leaves. Callus was successfully initiated from hairy roots on both Murashige-Skoog (MS) and Woody plants (WP) media containing (0.4 and 0.5 mg. L-1 thidiazuron(TDZ). Shoot regeneration occurred spontaneously. Also, the callus originated from hairy roots showed ability of shooting. Genetic transformation was detected by polymerase chain reaction (PCR). Alterations in anatomical structure of stems and leaves in transformed plants were observed. Transgenic plants' epidermal cell thickness was 14.3 µm rather than 12 µm. There are nine vascular bundles in regenerated plants that are open collateral vascular bundles. The palisade tissue thickness of transformed leaves was 96 µm, while 66 µm in non-transformed. It is clear that the genetic transformation led to anatomical changes of this medicinal plant. |
| format | Article |
| id | doaj-art-a0cda959add84c80a539e5a069fec4d5 |
| institution | DOAJ |
| issn | 1815-316X 2224-9796 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | College of Agriculture |
| record_format | Article |
| series | Mesopotamia Journal of Agriculture |
| spelling | doaj-art-a0cda959add84c80a539e5a069fec4d52025-08-20T02:56:51ZengCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962023-12-0151413014310.33899/mja.2023.143910.1280181687Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plantsRaghad Abdullah0Rasha Al-Jirjees1Shifa Salih2Department of Biology, College of Education for Pure Sciences, University of Mosul, Mosul, IraqDepartment of Biology, College of Education for Pure Sciences, University of Mosul, Mosul, IraqDepartment of Biology, College of Education for Pure Sciences, University of Mosul, Mosul, IraqThis study is conducted to find out the effect of genetic transformation by Agrobacterium rhizogenes ATCC13332 on the anatomical features of Eruca sativa. Stem explants and leaves were inoculated by direct injection of A. rhizogenes. The results indicated the high response of E. sativa, as the percentage of hairy roots formation was 95.3% for leaves. Callus was successfully initiated from hairy roots on both Murashige-Skoog (MS) and Woody plants (WP) media containing (0.4 and 0.5 mg. L-1 thidiazuron(TDZ). Shoot regeneration occurred spontaneously. Also, the callus originated from hairy roots showed ability of shooting. Genetic transformation was detected by polymerase chain reaction (PCR). Alterations in anatomical structure of stems and leaves in transformed plants were observed. Transgenic plants' epidermal cell thickness was 14.3 µm rather than 12 µm. There are nine vascular bundles in regenerated plants that are open collateral vascular bundles. The palisade tissue thickness of transformed leaves was 96 µm, while 66 µm in non-transformed. It is clear that the genetic transformation led to anatomical changes of this medicinal plant.https://magrj.mosuljournals.com/article_181687_fd85cb3b8c407b94cad511463384ef6d.pdfcallushairy rootsleavestransformation |
| spellingShingle | Raghad Abdullah Rasha Al-Jirjees Shifa Salih Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants Mesopotamia Journal of Agriculture callus hairy roots leaves transformation |
| title | Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants |
| title_full | Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants |
| title_fullStr | Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants |
| title_full_unstemmed | Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants |
| title_short | Effect of genetic transformation via Agrobacterium rhizogenes on the anatomical characteristics of Eruca Sativa Mill. plants |
| title_sort | effect of genetic transformation via agrobacterium rhizogenes on the anatomical characteristics of eruca sativa mill plants |
| topic | callus hairy roots leaves transformation |
| url | https://magrj.mosuljournals.com/article_181687_fd85cb3b8c407b94cad511463384ef6d.pdf |
| work_keys_str_mv | AT raghadabdullah effectofgenetictransformationviaagrobacteriumrhizogenesontheanatomicalcharacteristicsoferucasativamillplants AT rashaaljirjees effectofgenetictransformationviaagrobacteriumrhizogenesontheanatomicalcharacteristicsoferucasativamillplants AT shifasalih effectofgenetictransformationviaagrobacteriumrhizogenesontheanatomicalcharacteristicsoferucasativamillplants |