Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats
The functions of mesenchymal stem cells (MSCs) appear to decline with age due to cellular senescence, which could reduce the efficacy of MSCs-based therapies. Recently, MSCs have been identified in the nucleus pulposus, which offers great potential for intervertebral disc (IVD) repair. However, this...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/6761572 |
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| author | Yachao Zhao Zhiwei Jia Shanshan Huang Yaohong Wu Longgang Liu Linghan Lin Deli Wang Qing He Dike Ruan |
| author_facet | Yachao Zhao Zhiwei Jia Shanshan Huang Yaohong Wu Longgang Liu Linghan Lin Deli Wang Qing He Dike Ruan |
| author_sort | Yachao Zhao |
| collection | DOAJ |
| description | The functions of mesenchymal stem cells (MSCs) appear to decline with age due to cellular senescence, which could reduce the efficacy of MSCs-based therapies. Recently, MSCs have been identified in the nucleus pulposus, which offers great potential for intervertebral disc (IVD) repair. However, this potential might be affected by the senescence of nucleus pulposus MSCs (NPMSCs), but whether or not this exists remains unknown. The aim of this study was to investigate the age-related changes in NPMSCs. NPMSCs isolated from young (3-month-old) and old (14-month-old) Sprague-Dawley rats were cultured in vitro. Differences in morphology, proliferation, colony formation, multilineage differentiation, cell cycle, and expression of β-galactosidase (SA-β-gal) and senescent markers (p53, p21, and p16) were compared between groups. Both young and old NPMSCs fulfilled the criteria for definition as MSCs. Moreover, young NPMSCs presented better proliferation, colony-forming, and multilineage differentiation capacities than old NPMSCs. Old NPMSCs displayed senescent features, including significantly increased G0/G1 phase arrest, increased SA-β-gal expression, decreased S phase entry, and significant p53-p21-pRB pathway activation. Therefore, this is the first study demonstrating that senescent NPMSCs accumulate in IVD with age. The efficacy of NPMSCs is compromised by donor age, which should be taken into consideration prior to clinical application. |
| format | Article |
| id | doaj-art-a0a52b305b2345ccbedd4350fefb8129 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a0a52b305b2345ccbedd4350fefb81292025-02-03T06:08:13ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/67615726761572Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in RatsYachao Zhao0Zhiwei Jia1Shanshan Huang2Yaohong Wu3Longgang Liu4Linghan Lin5Deli Wang6Qing He7Dike Ruan8Department of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, The 306th Hospital of People’s Liberation Army, Beijing, ChinaDepartment of Laboratory Medicine, Children’s Hospital of Hebei Province, Shijiazhuang, Hebei, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaDepartment of Orthopaedics, Navy General Hospital, Beijing, ChinaThe functions of mesenchymal stem cells (MSCs) appear to decline with age due to cellular senescence, which could reduce the efficacy of MSCs-based therapies. Recently, MSCs have been identified in the nucleus pulposus, which offers great potential for intervertebral disc (IVD) repair. However, this potential might be affected by the senescence of nucleus pulposus MSCs (NPMSCs), but whether or not this exists remains unknown. The aim of this study was to investigate the age-related changes in NPMSCs. NPMSCs isolated from young (3-month-old) and old (14-month-old) Sprague-Dawley rats were cultured in vitro. Differences in morphology, proliferation, colony formation, multilineage differentiation, cell cycle, and expression of β-galactosidase (SA-β-gal) and senescent markers (p53, p21, and p16) were compared between groups. Both young and old NPMSCs fulfilled the criteria for definition as MSCs. Moreover, young NPMSCs presented better proliferation, colony-forming, and multilineage differentiation capacities than old NPMSCs. Old NPMSCs displayed senescent features, including significantly increased G0/G1 phase arrest, increased SA-β-gal expression, decreased S phase entry, and significant p53-p21-pRB pathway activation. Therefore, this is the first study demonstrating that senescent NPMSCs accumulate in IVD with age. The efficacy of NPMSCs is compromised by donor age, which should be taken into consideration prior to clinical application.http://dx.doi.org/10.1155/2017/6761572 |
| spellingShingle | Yachao Zhao Zhiwei Jia Shanshan Huang Yaohong Wu Longgang Liu Linghan Lin Deli Wang Qing He Dike Ruan Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats Stem Cells International |
| title | Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats |
| title_full | Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats |
| title_fullStr | Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats |
| title_full_unstemmed | Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats |
| title_short | Age-Related Changes in Nucleus Pulposus Mesenchymal Stem Cells: An In Vitro Study in Rats |
| title_sort | age related changes in nucleus pulposus mesenchymal stem cells an in vitro study in rats |
| url | http://dx.doi.org/10.1155/2017/6761572 |
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