Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells
Metastasis is the primary cause of death in patients with malignant tumors. Therefore, effectively controlling or reversing tumor cell growth and metastasis is crucial for treating malignant tumors. In this study, we investigated the effects and underlying mechanisms of Binpu-3 (a strain of Taraxacu...
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Frontiers Media S.A.
2025-03-01
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| author | Jiawei Wu Jianbo Zhang Wanyu Shu Wei Feng Ran Meng Lingyu Kong Huijuan Cao Chunhua Jiang Sitong Wang Fanwu Wu Chenxi Wu Chenxi Wu Xiuping Wang |
| author_facet | Jiawei Wu Jianbo Zhang Wanyu Shu Wei Feng Ran Meng Lingyu Kong Huijuan Cao Chunhua Jiang Sitong Wang Fanwu Wu Chenxi Wu Chenxi Wu Xiuping Wang |
| author_sort | Jiawei Wu |
| collection | DOAJ |
| description | Metastasis is the primary cause of death in patients with malignant tumors. Therefore, effectively controlling or reversing tumor cell growth and metastasis is crucial for treating malignant tumors. In this study, we investigated the effects and underlying mechanisms of Binpu-3 (a strain of Taraxacum sinicum Kitag., which was cultivated in slightly saline-alkali soil) on tumor invasion both in Drosophila and human breast cancer cells. High-performance liquid chromatography (HPLC) analysis revealed that caftaric, chlorogenic, caffeic, and cichoric acids in the Binpu-3 leaves and roots were significantly higher than those in the wild-type Handan strain. Binpu-3 root extract (Binpu-3RE) suppressed the invasion rate of tumor cells at 25.00 mg/mL in the Drosophila eyeful model, whereas Binpu-3 leaf extract had no obvious effect on tumor metastasis. Accordingly, we found that caffeic acid, quercetin, apigenin, and taraxasterol content in Binpu-3 roots was significantly higher than that in the leaves. In addition, ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) analysis revealed that Binpu-3RE contained various constituents, including pantothenate (0.1%), butein (0.53%), chlorogenate (0.78%), chicoric acid (1.96%), azelaic acid (0.23%), and [6]-gingerol (0.13%). In vivo, Binpu-3RE impeded ptc>scrib-IR triggered cell migration in Drosophila at an appropriate concentration, and 25.00 mg/mL was selected as the best dose to carry out follow-up mechanistic research. This dose of Binpu-3RE reduced the mRNA levels of Notch pathway key genes Delta, Serrate, Notch, Su(H), and En(spl), the expression levels of NRE-GFP (Notch activity reporter), β-integrin, and metalloproteinase-1 (MMP1) in Drosophila. Cell viability, wound healing, transwell, and Western blotting assays data implied that Binpu-3RE reduced cell growth, migration, invasion, and the expression of Notch1, Jagged1, and HES1 in human breast cancer MDA-MB-231 cells. In summary, the saline-alkali tolerant dandelion Binpu-3 used in this study was of excellent quality, and the root extract showed significant anti-tumor metastasis effects via reduction of Notch signal activity and the expression β-integrin and MMP1 proteins in Drosophila and breast cancer cells, providing a theoretical basis for the development and use of alkaline-soil dandelion herbs, and a therapeutic strategy for the clinical treatment of malignant breast cancer. |
| format | Article |
| id | doaj-art-a0706045cf1b46daa777f8ed504b51ab |
| institution | DOAJ |
| issn | 1663-9812 |
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| publishDate | 2025-03-01 |
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| spelling | doaj-art-a0706045cf1b46daa777f8ed504b51ab2025-08-20T02:52:56ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-03-011610.3389/fphar.2025.14945451494545Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cellsJiawei Wu0Jianbo Zhang1Wanyu Shu2Wei Feng3Ran Meng4Lingyu Kong5Huijuan Cao6Chunhua Jiang7Sitong Wang8Fanwu Wu9Chenxi Wu10Chenxi Wu11Xiuping Wang12Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaInstitute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, ChinaInstitute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, ChinaOncology of Chinese and Western Medicine, North China University of Science and Technology Affiliated Hospital, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications, College of Traditional Chinese Medicine, North China University of Science and Technology, Tangshan, ChinaHebei Key Laboratory of Medical Engineering and Integrated Utilization of Saline Alkali Land, Hebei Administration of TCM Key Laboratory of Quality Control of Salt Alkali Resistant TCM, Tangshan, ChinaInstitute of Coastal Agriculture, Hebei Academy of Agriculture and Forestry Sciences, Tangshan, ChinaMetastasis is the primary cause of death in patients with malignant tumors. Therefore, effectively controlling or reversing tumor cell growth and metastasis is crucial for treating malignant tumors. In this study, we investigated the effects and underlying mechanisms of Binpu-3 (a strain of Taraxacum sinicum Kitag., which was cultivated in slightly saline-alkali soil) on tumor invasion both in Drosophila and human breast cancer cells. High-performance liquid chromatography (HPLC) analysis revealed that caftaric, chlorogenic, caffeic, and cichoric acids in the Binpu-3 leaves and roots were significantly higher than those in the wild-type Handan strain. Binpu-3 root extract (Binpu-3RE) suppressed the invasion rate of tumor cells at 25.00 mg/mL in the Drosophila eyeful model, whereas Binpu-3 leaf extract had no obvious effect on tumor metastasis. Accordingly, we found that caffeic acid, quercetin, apigenin, and taraxasterol content in Binpu-3 roots was significantly higher than that in the leaves. In addition, ultra performance liquid chromatography-high resolution mass spectrometry (UPLC-HRMS) analysis revealed that Binpu-3RE contained various constituents, including pantothenate (0.1%), butein (0.53%), chlorogenate (0.78%), chicoric acid (1.96%), azelaic acid (0.23%), and [6]-gingerol (0.13%). In vivo, Binpu-3RE impeded ptc>scrib-IR triggered cell migration in Drosophila at an appropriate concentration, and 25.00 mg/mL was selected as the best dose to carry out follow-up mechanistic research. This dose of Binpu-3RE reduced the mRNA levels of Notch pathway key genes Delta, Serrate, Notch, Su(H), and En(spl), the expression levels of NRE-GFP (Notch activity reporter), β-integrin, and metalloproteinase-1 (MMP1) in Drosophila. Cell viability, wound healing, transwell, and Western blotting assays data implied that Binpu-3RE reduced cell growth, migration, invasion, and the expression of Notch1, Jagged1, and HES1 in human breast cancer MDA-MB-231 cells. In summary, the saline-alkali tolerant dandelion Binpu-3 used in this study was of excellent quality, and the root extract showed significant anti-tumor metastasis effects via reduction of Notch signal activity and the expression β-integrin and MMP1 proteins in Drosophila and breast cancer cells, providing a theoretical basis for the development and use of alkaline-soil dandelion herbs, and a therapeutic strategy for the clinical treatment of malignant breast cancer.https://www.frontiersin.org/articles/10.3389/fphar.2025.1494545/fullTaraxacum sinicum Kitag.root extracttumor invasionNotchDrosophilabreast cancer |
| spellingShingle | Jiawei Wu Jianbo Zhang Wanyu Shu Wei Feng Ran Meng Lingyu Kong Huijuan Cao Chunhua Jiang Sitong Wang Fanwu Wu Chenxi Wu Chenxi Wu Xiuping Wang Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells Frontiers in Pharmacology Taraxacum sinicum Kitag. root extract tumor invasion Notch Drosophila breast cancer |
| title | Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells |
| title_full | Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells |
| title_fullStr | Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells |
| title_full_unstemmed | Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells |
| title_short | Taraxacum sinicum Kitag. (Binpu-3) root extract inhibits tumor invasion via Notch signaling in Drosophila and human breast cancer MDA-MB-231 cells |
| title_sort | taraxacum sinicum kitag binpu 3 root extract inhibits tumor invasion via notch signaling in drosophila and human breast cancer mda mb 231 cells |
| topic | Taraxacum sinicum Kitag. root extract tumor invasion Notch Drosophila breast cancer |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1494545/full |
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