Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms
Background Periodontitis is not always satisfactorily treated with conventional scaling and root planing, and adjunctive use of antibiotics is required in clinical practice. Therefore, it is important for clinicians to understand the diversity and the antibiotic resistance of subgingival microbiota...
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PeerJ Inc.
2025-01-01
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| author | Qian Zhang Min Zhen Xiaochen Wang FengXiang Zhao Yang Dong Xiaoya Wang Shengtao Gao Jinfeng Wang Wenyu Shi Yifei Zhang |
| author_facet | Qian Zhang Min Zhen Xiaochen Wang FengXiang Zhao Yang Dong Xiaoya Wang Shengtao Gao Jinfeng Wang Wenyu Shi Yifei Zhang |
| author_sort | Qian Zhang |
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| description | Background Periodontitis is not always satisfactorily treated with conventional scaling and root planing, and adjunctive use of antibiotics is required in clinical practice. Therefore, it is important for clinicians to understand the diversity and the antibiotic resistance of subgingival microbiota when exposed to different antibiotics. Materials and Methods In this study, subgingival plaques were collected from 10 periodontitis patients and 11 periodontally healthy volunteers, and their microbiota response to selective pressure of four antibiotics (amoxicillin, metronidazole, clindamycin, and tetracycline) were evaluated through 16S rRNA gene amplicon and metagenomic sequencing analysis. Additionally, sensitive and resistant strains were isolated and cultured in vitro for resistance evaluation. Results Cultivation of subgingival microbiota revealed the oral microbiota from periodontitis patients were more resistant to antibiotics than that of healthy. Significant differences were also observed for the microbial community between with and without antibiotics (especially amoxicillin and tetracycline) treated in periodontitis group. Conclusion Overall, after the two antibiotics (amoxicillin and tetracycline) exposed, the oral subgingival microbiota in periodontitis patients exhibited different diversity and composition. Streptococcus may account for oral biofilm-specific antibiotic resistance in periodontitis. This provides information for personalized treatment of periodontitis. |
| format | Article |
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| institution | Kabale University |
| issn | 2167-8359 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj-art-a0697094dbdc432c89ebaf435dc966e62025-01-22T15:05:21ZengPeerJ Inc.PeerJ2167-83592025-01-0113e1883510.7717/peerj.18835Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilmsQian Zhang0Min Zhen1Xiaochen Wang2FengXiang Zhao3Yang Dong4Xiaoya Wang5Shengtao Gao6Jinfeng Wang7Wenyu Shi8Yifei Zhang9Central Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaDepartment of Periodontology, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaCAS Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing, ChinaCollege of Food Science & Nutritional Engineering, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, College of Biological Sciences, China Agricultural University, Beijing, ChinaDepartment of Dental Materials, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, ChinaBackground Periodontitis is not always satisfactorily treated with conventional scaling and root planing, and adjunctive use of antibiotics is required in clinical practice. Therefore, it is important for clinicians to understand the diversity and the antibiotic resistance of subgingival microbiota when exposed to different antibiotics. Materials and Methods In this study, subgingival plaques were collected from 10 periodontitis patients and 11 periodontally healthy volunteers, and their microbiota response to selective pressure of four antibiotics (amoxicillin, metronidazole, clindamycin, and tetracycline) were evaluated through 16S rRNA gene amplicon and metagenomic sequencing analysis. Additionally, sensitive and resistant strains were isolated and cultured in vitro for resistance evaluation. Results Cultivation of subgingival microbiota revealed the oral microbiota from periodontitis patients were more resistant to antibiotics than that of healthy. Significant differences were also observed for the microbial community between with and without antibiotics (especially amoxicillin and tetracycline) treated in periodontitis group. Conclusion Overall, after the two antibiotics (amoxicillin and tetracycline) exposed, the oral subgingival microbiota in periodontitis patients exhibited different diversity and composition. Streptococcus may account for oral biofilm-specific antibiotic resistance in periodontitis. This provides information for personalized treatment of periodontitis.https://peerj.com/articles/18835.pdfPeriodontitisOral microbiomeStrain isolationStreptococcusAntibiotic resistance |
| spellingShingle | Qian Zhang Min Zhen Xiaochen Wang FengXiang Zhao Yang Dong Xiaoya Wang Shengtao Gao Jinfeng Wang Wenyu Shi Yifei Zhang Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms PeerJ Periodontitis Oral microbiome Strain isolation Streptococcus Antibiotic resistance |
| title | Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| title_full | Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| title_fullStr | Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| title_full_unstemmed | Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| title_short | Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| title_sort | antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms |
| topic | Periodontitis Oral microbiome Strain isolation Streptococcus Antibiotic resistance |
| url | https://peerj.com/articles/18835.pdf |
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