Persistence of Brucella abortus in the Bone Marrow of Infected Mice
Brucellosis is a zoonotic bacterial infection that may persist for long periods causing relapses in antibiotic-treated patients. The ability of Brucella to develop chronic infections is linked to their capacity to invade and replicate within the mononuclear phagocyte system, including the bone marro...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/5370414 |
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| author | Cristina Gutiérrez-Jiménez Lisiena Hysenaj Alejandro Alfaro-Alarcón Ricardo Mora-Cartín Vilma Arce-Gorvel Edgardo Moreno Jean Pierre Gorvel Elías Barquero-Calvo |
| author_facet | Cristina Gutiérrez-Jiménez Lisiena Hysenaj Alejandro Alfaro-Alarcón Ricardo Mora-Cartín Vilma Arce-Gorvel Edgardo Moreno Jean Pierre Gorvel Elías Barquero-Calvo |
| author_sort | Cristina Gutiérrez-Jiménez |
| collection | DOAJ |
| description | Brucellosis is a zoonotic bacterial infection that may persist for long periods causing relapses in antibiotic-treated patients. The ability of Brucella to develop chronic infections is linked to their capacity to invade and replicate within the mononuclear phagocyte system, including the bone marrow (BM). Persistence of Brucella in the BM has been associated with hematological complications such as neutropenia, thrombocytopenia, anemia, and pancytopenia in human patients. In the mouse model, we observed that the number of Brucella abortus in the BM remained constant for up to 168 days of postinfection. This persistence was associated with histopathological changes, accompanied by augmented numbers of BM myeloid GMP progenitors, PMNs, and CD4+ lymphocytes during the acute phase (eight days) of the infection in the BM. Monocytes, PMNs, and GMP cells were identified as the cells harboring Brucella in the BM. We propose that the BM is an essential niche for the bacterium to establish long-lasting infections and that infected PMNs may serve as vehicles for dispersion of Brucella organisms, following the Trojan horse hypothesis. Monocytes are solid candidates for Brucella reservoirs in the BM. |
| format | Article |
| id | doaj-art-a060557173634c8dbb8e28c418956a40 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-a060557173634c8dbb8e28c418956a402025-02-03T05:53:41ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/53704145370414Persistence of Brucella abortus in the Bone Marrow of Infected MiceCristina Gutiérrez-Jiménez0Lisiena Hysenaj1Alejandro Alfaro-Alarcón2Ricardo Mora-Cartín3Vilma Arce-Gorvel4Edgardo Moreno5Jean Pierre Gorvel6Elías Barquero-Calvo7Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa RicaAix Marseille University, CNRS, INSERM, CIML, Marseille, FrancePathology Department, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa RicaPrograma de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa RicaAix Marseille University, CNRS, INSERM, CIML, Marseille, FrancePrograma de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa RicaAix Marseille University, CNRS, INSERM, CIML, Marseille, FrancePrograma de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa RicaBrucellosis is a zoonotic bacterial infection that may persist for long periods causing relapses in antibiotic-treated patients. The ability of Brucella to develop chronic infections is linked to their capacity to invade and replicate within the mononuclear phagocyte system, including the bone marrow (BM). Persistence of Brucella in the BM has been associated with hematological complications such as neutropenia, thrombocytopenia, anemia, and pancytopenia in human patients. In the mouse model, we observed that the number of Brucella abortus in the BM remained constant for up to 168 days of postinfection. This persistence was associated with histopathological changes, accompanied by augmented numbers of BM myeloid GMP progenitors, PMNs, and CD4+ lymphocytes during the acute phase (eight days) of the infection in the BM. Monocytes, PMNs, and GMP cells were identified as the cells harboring Brucella in the BM. We propose that the BM is an essential niche for the bacterium to establish long-lasting infections and that infected PMNs may serve as vehicles for dispersion of Brucella organisms, following the Trojan horse hypothesis. Monocytes are solid candidates for Brucella reservoirs in the BM.http://dx.doi.org/10.1155/2018/5370414 |
| spellingShingle | Cristina Gutiérrez-Jiménez Lisiena Hysenaj Alejandro Alfaro-Alarcón Ricardo Mora-Cartín Vilma Arce-Gorvel Edgardo Moreno Jean Pierre Gorvel Elías Barquero-Calvo Persistence of Brucella abortus in the Bone Marrow of Infected Mice Journal of Immunology Research |
| title | Persistence of Brucella abortus in the Bone Marrow of Infected Mice |
| title_full | Persistence of Brucella abortus in the Bone Marrow of Infected Mice |
| title_fullStr | Persistence of Brucella abortus in the Bone Marrow of Infected Mice |
| title_full_unstemmed | Persistence of Brucella abortus in the Bone Marrow of Infected Mice |
| title_short | Persistence of Brucella abortus in the Bone Marrow of Infected Mice |
| title_sort | persistence of brucella abortus in the bone marrow of infected mice |
| url | http://dx.doi.org/10.1155/2018/5370414 |
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