Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates
Seed watermelon kernel is a typical complex food with high fat and protein contents. During storage and processing, it is often affected by various factors to undergo interactions between components, which lead to its quality change. In this experiment, seed watermelon kernels were used as research...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2020-12-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301725 |
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| author | Shugang Li Zhihao Li Xiuting Li Ping Wang Xiongwei Yu Qinli Fu Sihai Gao |
| author_facet | Shugang Li Zhihao Li Xiuting Li Ping Wang Xiongwei Yu Qinli Fu Sihai Gao |
| author_sort | Shugang Li |
| collection | DOAJ |
| description | Seed watermelon kernel is a typical complex food with high fat and protein contents. During storage and processing, it is often affected by various factors to undergo interactions between components, which lead to its quality change. In this experiment, seed watermelon kernels were used as research objects, and the effects of 2′-Azobis (2-amidinopropane) dihydrochloride (AAPH) on seed watermelon kernel protein isolates (WMP) were investigated. The structure and digestion characteristics of WMP after oxidation were studied. The results showed that with the increase of AAPH concentration (0.05−5 mol/L), WMP showed obvious aggregation, and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL. The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g, α-helix decreased and β-sheet decreased in secondary structure, and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g, enthalpy (ΔH) and denaturation temperature increased (Td) (P < 0.05). By mass spectrometry results of simulated gastric digestion products, it was found that oxidation adversely affected the digestion characteristics of WMP. It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel. |
| format | Article |
| id | doaj-art-a04e384997de4d01a9013c48183ed16d |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-a04e384997de4d01a9013c48183ed16d2025-02-03T11:06:08ZengTsinghua University PressFood Science and Human Wellness2213-45302020-12-0194402410Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolatesShugang Li0Zhihao Li1Xiuting Li2Ping Wang3Xiongwei Yu4Qinli Fu5Sihai Gao6School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; Key Laboratory of Fermentation Engineering, Ministry of Education, Glyn O. Phillips Hydrophilic Colloid Research Center, Faculty of Light Industry, School of Food and Biological Engineering, Hubei University of Technology, Wuhan, 430068, ChinaKey Laboratory of Fermentation Engineering, Ministry of Education, Glyn O. Phillips Hydrophilic Colloid Research Center, Faculty of Light Industry, School of Food and Biological Engineering, Hubei University of Technology, Wuhan, 430068, ChinaBeijing Technology and Business University, Beijing, 100048, ChinaHuazhong Agriculture University, Wuhan, 430070, ChinaWuhan Xudong Food Co., Ltd., Wuhan 430000, ChinaWuhan Xudong Food Co., Ltd., Wuhan 430000, ChinaDepartment of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China; Corresponding author.Seed watermelon kernel is a typical complex food with high fat and protein contents. During storage and processing, it is often affected by various factors to undergo interactions between components, which lead to its quality change. In this experiment, seed watermelon kernels were used as research objects, and the effects of 2′-Azobis (2-amidinopropane) dihydrochloride (AAPH) on seed watermelon kernel protein isolates (WMP) were investigated. The structure and digestion characteristics of WMP after oxidation were studied. The results showed that with the increase of AAPH concentration (0.05−5 mol/L), WMP showed obvious aggregation, and its solubility decreased from 6.76 mg/mL to 9.59 mg/mL. The free sulfhydryl content of WMP was 18.24 mmol/g decreased to 11.25 mmol/g, α-helix decreased and β-sheet decreased in secondary structure, and its disulfide bond increased by 43.06 mmol/g from 39.57 mmol/g, enthalpy (ΔH) and denaturation temperature increased (Td) (P < 0.05). By mass spectrometry results of simulated gastric digestion products, it was found that oxidation adversely affected the digestion characteristics of WMP. It can be seen that the lipid oxidation product APPH of seed watermelon kernel can significantly affect the structure and function of the protein extracted from the seed kernel.http://www.sciencedirect.com/science/article/pii/S2213453020301725Seed watermelon kernel proteinDigestion characteristicsAAPH oxidation |
| spellingShingle | Shugang Li Zhihao Li Xiuting Li Ping Wang Xiongwei Yu Qinli Fu Sihai Gao Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates Food Science and Human Wellness Seed watermelon kernel protein Digestion characteristics AAPH oxidation |
| title | Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates |
| title_full | Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates |
| title_fullStr | Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates |
| title_full_unstemmed | Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates |
| title_short | Effect of AAPH oxidation on digestion characteristics of seed watermelon (Citrullus lanatus var) kernels protein isolates |
| title_sort | effect of aaph oxidation on digestion characteristics of seed watermelon citrullus lanatus var kernels protein isolates |
| topic | Seed watermelon kernel protein Digestion characteristics AAPH oxidation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301725 |
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