Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease
Background. Pediatric inflammatory bowel disease (IBD) is on the rise worldwide. Endoscopies are necessary for IBD assessment but are invasive, expensive, and inconvenient. Recently, fecal calprotectin (FCal) was proposed as a noninvasive and specific marker of gut inflammation. We evaluated the ana...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Canadian Journal of Gastroenterology and Hepatology |
| Online Access: | http://dx.doi.org/10.1155/2017/1450970 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565115002552320 |
|---|---|
| author | Saranya Kittanakom Md. Sharif Shajib Kristine Garvie Joceline Turner Dan Brooks Sufian Odeh Robert Issenman V. Tony Chetty Joseph Macri Waliul I. Khan |
| author_facet | Saranya Kittanakom Md. Sharif Shajib Kristine Garvie Joceline Turner Dan Brooks Sufian Odeh Robert Issenman V. Tony Chetty Joseph Macri Waliul I. Khan |
| author_sort | Saranya Kittanakom |
| collection | DOAJ |
| description | Background. Pediatric inflammatory bowel disease (IBD) is on the rise worldwide. Endoscopies are necessary for IBD assessment but are invasive, expensive, and inconvenient. Recently, fecal calprotectin (FCal) was proposed as a noninvasive and specific marker of gut inflammation. We evaluated the analytical performance of three FCal assays and their clinical performance in predicting relapse in pediatric IBD. Methods. This study used 40 pediatric IBD and 40 random non-IBD patients’ fecal samples. Two automated ELISAs (Bühlmann and PhiCal® Calprotectin-EIA) and an EliA (Phadia 250 EliA-Calprotectin) were used to evaluate the analytical performance. The clinical performance was assessed by PhiCal Calprotectin-EIA, EliA-Calprotectin, and Bühlmann immunochromatographic point-of-care test (POCT). Results. All assays displayed acceptable analytical performance below and above the medical decision cut-off [imprecision (CV < 10% intra-assay; <15% interassay); linearity (overall mean % deviation < 16.5%)]. The agreement with PhiCal Calprotectin-EIA was 100% and 78.6% for Bühlmann (95% CI, 87.5–100; Kappa: 1) and EliA-Calprotectin (95% CI, 60.5–89.8; Kappa: 0.32), respectively, and 63.6% between Bühlmann and EliA-Calprotectin (95% CI, 46.6–77.8; Kappa: 0.16). All assays evaluated had similar clinical performance [AUC: 0.84 (EliA-Calprotectin); 0.83 (POCT and PhiCal Calprotectin-EIA)]. Conclusion. FCal levels determined using the same method and assay together with clinical history would be a noninvasive and useful tool in monitoring pediatric IBD. |
| format | Article |
| id | doaj-art-a0397ba9cec04afb8b866a8a26ad1466 |
| institution | Kabale University |
| issn | 2291-2789 2291-2797 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology and Hepatology |
| spelling | doaj-art-a0397ba9cec04afb8b866a8a26ad14662025-02-03T01:09:25ZengWileyCanadian Journal of Gastroenterology and Hepatology2291-27892291-27972017-01-01201710.1155/2017/14509701450970Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel DiseaseSaranya Kittanakom0Md. Sharif Shajib1Kristine Garvie2Joceline Turner3Dan Brooks4Sufian Odeh5Robert Issenman6V. Tony Chetty7Joseph Macri8Waliul I. Khan9Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, L8S 4K1, CanadaHamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, ON, CanadaHamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, ON, CanadaHamilton Regional Laboratory Medicine Program, Hamilton Health Sciences, Hamilton, ON, CanadaDivision of Pediatric Gastroenterology, McMaster University, Hamilton, ON, CanadaFarncombe Family Digestive Health Research Institute, Hamilton, ON, CanadaDepartment of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, L8S 4K1, CanadaDepartment of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, L8S 4K1, CanadaBackground. Pediatric inflammatory bowel disease (IBD) is on the rise worldwide. Endoscopies are necessary for IBD assessment but are invasive, expensive, and inconvenient. Recently, fecal calprotectin (FCal) was proposed as a noninvasive and specific marker of gut inflammation. We evaluated the analytical performance of three FCal assays and their clinical performance in predicting relapse in pediatric IBD. Methods. This study used 40 pediatric IBD and 40 random non-IBD patients’ fecal samples. Two automated ELISAs (Bühlmann and PhiCal® Calprotectin-EIA) and an EliA (Phadia 250 EliA-Calprotectin) were used to evaluate the analytical performance. The clinical performance was assessed by PhiCal Calprotectin-EIA, EliA-Calprotectin, and Bühlmann immunochromatographic point-of-care test (POCT). Results. All assays displayed acceptable analytical performance below and above the medical decision cut-off [imprecision (CV < 10% intra-assay; <15% interassay); linearity (overall mean % deviation < 16.5%)]. The agreement with PhiCal Calprotectin-EIA was 100% and 78.6% for Bühlmann (95% CI, 87.5–100; Kappa: 1) and EliA-Calprotectin (95% CI, 60.5–89.8; Kappa: 0.32), respectively, and 63.6% between Bühlmann and EliA-Calprotectin (95% CI, 46.6–77.8; Kappa: 0.16). All assays evaluated had similar clinical performance [AUC: 0.84 (EliA-Calprotectin); 0.83 (POCT and PhiCal Calprotectin-EIA)]. Conclusion. FCal levels determined using the same method and assay together with clinical history would be a noninvasive and useful tool in monitoring pediatric IBD.http://dx.doi.org/10.1155/2017/1450970 |
| spellingShingle | Saranya Kittanakom Md. Sharif Shajib Kristine Garvie Joceline Turner Dan Brooks Sufian Odeh Robert Issenman V. Tony Chetty Joseph Macri Waliul I. Khan Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease Canadian Journal of Gastroenterology and Hepatology |
| title | Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease |
| title_full | Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease |
| title_fullStr | Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease |
| title_full_unstemmed | Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease |
| title_short | Comparison of Fecal Calprotectin Methods for Predicting Relapse of Pediatric Inflammatory Bowel Disease |
| title_sort | comparison of fecal calprotectin methods for predicting relapse of pediatric inflammatory bowel disease |
| url | http://dx.doi.org/10.1155/2017/1450970 |
| work_keys_str_mv | AT saranyakittanakom comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT mdsharifshajib comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT kristinegarvie comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT jocelineturner comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT danbrooks comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT sufianodeh comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT robertissenman comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT vtonychetty comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT josephmacri comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease AT waliulikhan comparisonoffecalcalprotectinmethodsforpredictingrelapseofpediatricinflammatoryboweldisease |