DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES
Due to the lack of effective tools of ASF specific prevention it is evident that early diagnosis is one of the most important and resultative ways of the disease control. However, contemporary diagnosis is a complex component of any effective surveillance system. Latest scientific achievements facil...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2019-06-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/412 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832086748754083840 |
|---|---|
| author | D. V. Sharypova O. V. Kapustina I. Yu. Zhukov N. N. Vlasova A. S. Igolkin |
| author_facet | D. V. Sharypova O. V. Kapustina I. Yu. Zhukov N. N. Vlasova A. S. Igolkin |
| author_sort | D. V. Sharypova |
| collection | DOAJ |
| description | Due to the lack of effective tools of ASF specific prevention it is evident that early diagnosis is one of the most important and resultative ways of the disease control. However, contemporary diagnosis is a complex component of any effective surveillance system. Latest scientific achievements facilitated not only highly specific and sensitive but also rapid methods of laboratory diagnosis. Nevertheless, further development, improvement and expansion of ASF diagnosis techniques including rapid tests is a topical task of a great concern. The research is devoted to development of rapid test methods for rapid detection of antibodies to ASFV in blood sera of infected animals as well as to analysis of their use effectiveness. The following methods were suggested: immunoperoxidase monolayer assay using fixed cell line (ASFV permissive CV-1 cell-line infected with the virus strain ASF/ARRIAH/CV-1) and latex agglutination test using ASFV p30 recombinant protein. The performed research demonstrated the effectiveness of the applied techniques for ASF serological diagnosis. Latex agglutination test and immunoperoxidase monolayer assay give rapid and high quality test results (within 1–2 hours). The advantage of the specified methods as compared to ELISA is their simplicity and the possibility of use in conditions of limited technical support. |
| format | Article |
| id | doaj-art-a034d200e1c747bfb81c9a3f05f8a5fd |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-a034d200e1c747bfb81c9a3f05f8a5fd2025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-06-0102303410.29326/2304-196X-2019-2-29-30-34405DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUESD. V. Sharypova0O. V. Kapustina1I. Yu. Zhukov2N. N. Vlasova3A. S. Igolkin4FGBI "ARRIAH", VladimirFGBSI FSC, FGBSI FNC VIEV RANFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirDue to the lack of effective tools of ASF specific prevention it is evident that early diagnosis is one of the most important and resultative ways of the disease control. However, contemporary diagnosis is a complex component of any effective surveillance system. Latest scientific achievements facilitated not only highly specific and sensitive but also rapid methods of laboratory diagnosis. Nevertheless, further development, improvement and expansion of ASF diagnosis techniques including rapid tests is a topical task of a great concern. The research is devoted to development of rapid test methods for rapid detection of antibodies to ASFV in blood sera of infected animals as well as to analysis of their use effectiveness. The following methods were suggested: immunoperoxidase monolayer assay using fixed cell line (ASFV permissive CV-1 cell-line infected with the virus strain ASF/ARRIAH/CV-1) and latex agglutination test using ASFV p30 recombinant protein. The performed research demonstrated the effectiveness of the applied techniques for ASF serological diagnosis. Latex agglutination test and immunoperoxidase monolayer assay give rapid and high quality test results (within 1–2 hours). The advantage of the specified methods as compared to ELISA is their simplicity and the possibility of use in conditions of limited technical support.https://veterinary.arriah.ru/jour/article/view/412african swine feverlatex agglutination testimmunoperoxidase monolayer assaycv-1 continuous cell linep30 recombinant proteinvirus antigen |
| spellingShingle | D. V. Sharypova O. V. Kapustina I. Yu. Zhukov N. N. Vlasova A. S. Igolkin DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES Ветеринария сегодня african swine fever latex agglutination test immunoperoxidase monolayer assay cv-1 continuous cell line p30 recombinant protein virus antigen |
| title | DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES |
| title_full | DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES |
| title_fullStr | DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES |
| title_full_unstemmed | DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES |
| title_short | DEVELOPMENT AND IMPROVEMENT OF ASF SEROLOGICAL DIAGNOSTIC TECHNIQUES |
| title_sort | development and improvement of asf serological diagnostic techniques |
| topic | african swine fever latex agglutination test immunoperoxidase monolayer assay cv-1 continuous cell line p30 recombinant protein virus antigen |
| url | https://veterinary.arriah.ru/jour/article/view/412 |
| work_keys_str_mv | AT dvsharypova developmentandimprovementofasfserologicaldiagnostictechniques AT ovkapustina developmentandimprovementofasfserologicaldiagnostictechniques AT iyuzhukov developmentandimprovementofasfserologicaldiagnostictechniques AT nnvlasova developmentandimprovementofasfserologicaldiagnostictechniques AT asigolkin developmentandimprovementofasfserologicaldiagnostictechniques |