Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics
The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability of A. absinthium L. aerial parts collected from four different Tunisian r...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/804658 |
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| author | Kamel Msaada Nidhal Salem Olfa Bachrouch Slim Bousselmi Sonia Tammar Abdulkhaleg Alfaify Khaldoun Al Sane Wided Ben Ammar Sana Azeiz Adel Haj Brahim Majdi Hammami Sawsen Selmi Ferid Limam Brahim Marzouk |
| author_facet | Kamel Msaada Nidhal Salem Olfa Bachrouch Slim Bousselmi Sonia Tammar Abdulkhaleg Alfaify Khaldoun Al Sane Wided Ben Ammar Sana Azeiz Adel Haj Brahim Majdi Hammami Sawsen Selmi Ferid Limam Brahim Marzouk |
| author_sort | Kamel Msaada |
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| description | The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability of A. absinthium L. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant. A. absinthium L. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities. |
| format | Article |
| id | doaj-art-a02520a8a2094e498adda52869b32975 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a02520a8a2094e498adda52869b329752025-02-03T01:10:47ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/804658804658Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and PhenolicsKamel Msaada0Nidhal Salem1Olfa Bachrouch2Slim Bousselmi3Sonia Tammar4Abdulkhaleg Alfaify5Khaldoun Al Sane6Wided Ben Ammar7Sana Azeiz8Adel Haj Brahim9Majdi Hammami10Sawsen Selmi11Ferid Limam12Brahim Marzouk13Laboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaBiology Department, College of Sciences, King Khalid University, P.O. Box 9004, Abha 61413, Saudi ArabiaBiology Department, College of Sciences, King Khalid University, P.O. Box 9004, Abha 61413, Saudi ArabiaUnit of Research “Nitrogenized Nutrition and Metabolism and Stress Proteins” UR 99/09-20, Department of Biology, Faculty of Science, University of Tunis, 2092 Tunis, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaLaboratory of Bioactive Substances, Biotechnology Center in Borj-Cedria Technopol, BP 901, 2050 Hammam-Lif, TunisiaThe aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability of A. absinthium L. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant. A. absinthium L. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.http://dx.doi.org/10.1155/2015/804658 |
| spellingShingle | Kamel Msaada Nidhal Salem Olfa Bachrouch Slim Bousselmi Sonia Tammar Abdulkhaleg Alfaify Khaldoun Al Sane Wided Ben Ammar Sana Azeiz Adel Haj Brahim Majdi Hammami Sawsen Selmi Ferid Limam Brahim Marzouk Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics Journal of Chemistry |
| title | Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics |
| title_full | Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics |
| title_fullStr | Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics |
| title_full_unstemmed | Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics |
| title_short | Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthium L.) Essential Oils and Phenolics |
| title_sort | chemical composition and antioxidant and antimicrobial activities of wormwood artemisia absinthium l essential oils and phenolics |
| url | http://dx.doi.org/10.1155/2015/804658 |
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