High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propag...
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| Format: | Article |
| Language: | English |
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Wiley
2006-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/IJBI/2006/93438 |
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| _version_ | 1832556348512927744 |
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| author | Daniel Day Charles G. Cranfield Min Gu |
| author_facet | Daniel Day Charles G. Cranfield Min Gu |
| author_sort | Daniel Day |
| collection | DOAJ |
| description | We have demonstrated a combined imaging system, where the
physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond
laser irradiation. Individual GH3 cells labeled with the calcium
fluorophore Fluo-3 were stimulated using a counter-propagating
focused femtosecond beam with respect to the imaging system. As a
result of the stimulation, calcium waves can be generated in COS
cells, and laser-induced calcium oscillations are initiated in the
GH3 cells. Single-photon fluorescence images and intensity
profiles of the targeted specimens are sampled in real-time using
a modified PerkinElmer UltraView LCI microscope. |
| format | Article |
| id | doaj-art-a01dba58badf4c239b02dd4e5f79a516 |
| institution | Kabale University |
| issn | 1687-4188 1687-4196 |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomedical Imaging |
| spelling | doaj-art-a01dba58badf4c239b02dd4e5f79a5162025-02-03T05:45:42ZengWileyInternational Journal of Biomedical Imaging1687-41881687-41962006-01-01200610.1155/IJBI/2006/9343893438High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium TransientsDaniel Day0Charles G. Cranfield1Min Gu2Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn VIC 3122, AustraliaCentre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn VIC 3122, AustraliaCentre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P.O. Box 218, Hawthorn VIC 3122, AustraliaWe have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope.http://dx.doi.org/10.1155/IJBI/2006/93438 |
| spellingShingle | Daniel Day Charles G. Cranfield Min Gu High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients International Journal of Biomedical Imaging |
| title | High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients |
| title_full | High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients |
| title_fullStr | High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients |
| title_full_unstemmed | High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients |
| title_short | High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients |
| title_sort | high speed fluorescence imaging and intensity profiling of femtosecond induced calcium transients |
| url | http://dx.doi.org/10.1155/IJBI/2006/93438 |
| work_keys_str_mv | AT danielday highspeedfluorescenceimagingandintensityprofilingoffemtosecondinducedcalciumtransients AT charlesgcranfield highspeedfluorescenceimagingandintensityprofilingoffemtosecondinducedcalciumtransients AT mingu highspeedfluorescenceimagingandintensityprofilingoffemtosecondinducedcalciumtransients |