High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients
We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propag...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
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| Series: | International Journal of Biomedical Imaging |
| Online Access: | http://dx.doi.org/10.1155/IJBI/2006/93438 |
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| Summary: | We have demonstrated a combined imaging system, where the
physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond
laser irradiation. Individual GH3 cells labeled with the calcium
fluorophore Fluo-3 were stimulated using a counter-propagating
focused femtosecond beam with respect to the imaging system. As a
result of the stimulation, calcium waves can be generated in COS
cells, and laser-induced calcium oscillations are initiated in the
GH3 cells. Single-photon fluorescence images and intensity
profiles of the targeted specimens are sampled in real-time using
a modified PerkinElmer UltraView LCI microscope. |
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| ISSN: | 1687-4188 1687-4196 |