Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression
The production of valuable bioactive compounds in the medicinal plant <i>Glycyrrhiza glabra</i> L. (<i>G. glabra</i>) would benefit from biotechnological approaches for the cultivation and induction of metabolite-producing hairy roots. Germination trials were tested to overco...
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MDPI AG
2025-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/11/1/62 |
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| author | Negin Afsharzadeh Renate Paltram Arne Jungwirth Leila Tabrizi Vahideh Nazeri Hadi Kalantari Heidi Halbwirth Leila Samiei Hester Sheehan Majid Shokrpour |
| author_facet | Negin Afsharzadeh Renate Paltram Arne Jungwirth Leila Tabrizi Vahideh Nazeri Hadi Kalantari Heidi Halbwirth Leila Samiei Hester Sheehan Majid Shokrpour |
| author_sort | Negin Afsharzadeh |
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| description | The production of valuable bioactive compounds in the medicinal plant <i>Glycyrrhiza glabra</i> L. (<i>G. glabra</i>) would benefit from biotechnological approaches for the cultivation and induction of metabolite-producing hairy roots. Germination trials were tested to overcome seed dormancy, achieving high germination rates with sulfuric acid treatment. Hairy root cultures of cotyledons using <i>Rhizobium rhizogenes</i> strain 1724 showed the highest transformation efficiency. A fast-growing line, line S, was subsequently exposed to light treatments (red, blue, and blue and red combined) to evaluate their effects on growth, phenolic content, and Ferric Reducing Antioxidant Power (FRAP). Hairy root cultures grown in blue light and in blue and red light combined had higher growth rates than those grown in red light only or in control conditions (dark). FRAP increased over time under all light treatments, including the control, and those cultures exposed to blue and red light combined had higher FRAP than the control. These findings provide valuable insights into conditions for optimal seed germination and hairy root transformation. Treatment of the line S with different qualities of light induced changes in antioxidant capacity and phenolic content, indicating promise for its use in upregulating secondary metabolite production in <i>G. glabra</i> for future biotechnological applications. |
| format | Article |
| id | doaj-art-9fd91a05583545b188541a39f907929d |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-9fd91a05583545b188541a39f907929d2025-01-24T13:34:39ZengMDPI AGHorticulturae2311-75242025-01-011116210.3390/horticulturae11010062Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite OverexpressionNegin Afsharzadeh0Renate Paltram1Arne Jungwirth2Leila Tabrizi3Vahideh Nazeri4Hadi Kalantari5Heidi Halbwirth6Leila Samiei7Hester Sheehan8Majid Shokrpour9Department of Horticultural Science and Landscape, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, IranInstitut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, 1060 Vienna, AustriaKonrad Lorenz Institute of Ethology, University of Veterinary Medicine Vienna, 1160 Vienna, AustriaDepartment of Horticultural Science and Landscape, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, IranDepartment of Horticultural Science and Landscape, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, IranMedicinal Plants Research Center, Institute of Medicinal Plants, Academic Center for Education, Culture and Research, Karaj 369-31375, IranInstitut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, 1060 Vienna, AustriaDepartment of Horticultural Science and Landscape, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad 9177948974, IranInstitut für Verfahrenstechnik, Umwelttechnik und Technische Biowissenschaften, Technische Universität Wien, 1060 Vienna, AustriaDepartment of Horticultural Science and Landscape, Faculty of Agriculture, University College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, IranThe production of valuable bioactive compounds in the medicinal plant <i>Glycyrrhiza glabra</i> L. (<i>G. glabra</i>) would benefit from biotechnological approaches for the cultivation and induction of metabolite-producing hairy roots. Germination trials were tested to overcome seed dormancy, achieving high germination rates with sulfuric acid treatment. Hairy root cultures of cotyledons using <i>Rhizobium rhizogenes</i> strain 1724 showed the highest transformation efficiency. A fast-growing line, line S, was subsequently exposed to light treatments (red, blue, and blue and red combined) to evaluate their effects on growth, phenolic content, and Ferric Reducing Antioxidant Power (FRAP). Hairy root cultures grown in blue light and in blue and red light combined had higher growth rates than those grown in red light only or in control conditions (dark). FRAP increased over time under all light treatments, including the control, and those cultures exposed to blue and red light combined had higher FRAP than the control. These findings provide valuable insights into conditions for optimal seed germination and hairy root transformation. Treatment of the line S with different qualities of light induced changes in antioxidant capacity and phenolic content, indicating promise for its use in upregulating secondary metabolite production in <i>G. glabra</i> for future biotechnological applications.https://www.mdpi.com/2311-7524/11/1/62<i>Glycyrrhiza glabra</i> L.seed germinationhairy rootlight inductionpolyphenolsantioxidant activity |
| spellingShingle | Negin Afsharzadeh Renate Paltram Arne Jungwirth Leila Tabrizi Vahideh Nazeri Hadi Kalantari Heidi Halbwirth Leila Samiei Hester Sheehan Majid Shokrpour Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression Horticulturae <i>Glycyrrhiza glabra</i> L. seed germination hairy root light induction polyphenols antioxidant activity |
| title | Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression |
| title_full | Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression |
| title_fullStr | Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression |
| title_full_unstemmed | Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression |
| title_short | Advancing <i>Glycyrrhiza glabra</i> L. Cultivation and Hairy Root Transformation and Elicitation for Future Metabolite Overexpression |
| title_sort | advancing i glycyrrhiza glabra i l cultivation and hairy root transformation and elicitation for future metabolite overexpression |
| topic | <i>Glycyrrhiza glabra</i> L. seed germination hairy root light induction polyphenols antioxidant activity |
| url | https://www.mdpi.com/2311-7524/11/1/62 |
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