Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer
Background: Proprotein convertases (PCs) are serine proteases that after restricted proteolysis activate many proteins that play a crucial role in cancer such as metalloproteinases, growth factors and growth factor receptors, adhesion molecules, and angiogenic factors. Although the expression of sev...
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| Format: | Article |
| Language: | English |
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Wiley
2007-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.1155/2007/930321 |
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| author | Robert E. Page Andrés J. P. Klein-Szanto Samuel Litwin Emmanuelle Nicolas Raid Al-Jumaily Peter Alexander Andrew K. Godwin Eric A. Ross Russell J. Schilder Daniel E. Bassi |
| author_facet | Robert E. Page Andrés J. P. Klein-Szanto Samuel Litwin Emmanuelle Nicolas Raid Al-Jumaily Peter Alexander Andrew K. Godwin Eric A. Ross Russell J. Schilder Daniel E. Bassi |
| author_sort | Robert E. Page |
| collection | DOAJ |
| description | Background: Proprotein convertases (PCs) are serine proteases that after restricted proteolysis activate many proteins that play a crucial role in cancer such as metalloproteinases, growth factors and growth factor receptors, adhesion molecules, and angiogenic factors. Although the expression of several PCs is increased in many tumors, their expression in primary ovarian tumors has not been studied in detail. We sought to determine if there was an association between the expression of the ubiquitously expressed PCs, furin, PACE-4, PC-5 and PC-7, and ovarian tumor progression. Methods: We assessed their expression by RT-PCR, Real-time PCR, Western blot, and immunohistochemistry using cells derived from normal human ovarian surface epithelium (HOSE) and cancer cell lines as well as ovarian epithelial cancer specimens (45 RT-PCR/Real-time PCR, and 120 archival specimens for Immunohistochemistry). Results: We found that furin expression was restricted to the cancer cell lines. In contrast, PACE-4 and PC-7 showed expression only in normal HOSE cells lines. Furthermore, furin was predominantly expressed in primary tumors from patients who survived for less than five years. The other PCs are either expressed in the group of survivors (PC-7 and PACE4) or expressed in low amounts (PC-5). Conclusions: Our studies point to a clear relationship between furin and ovarian cancer. In addition, these results show that furin exhibits the closest association with ovarian cancer among the ubiquitously expressed PCs, arguing against the redundancy of these proteases. In summary, furin may constitute a marker for ovarian tumor progression and could contribute to predict the outcome of this disease. |
| format | Article |
| id | doaj-art-9faf7f9114374427b3d720237c5d8ca0 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-9faf7f9114374427b3d720237c5d8ca02025-02-03T00:59:31ZengWileyCellular Oncology1570-58701875-86062007-01-0129428929910.1155/2007/930321Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian CancerRobert E. Page0Andrés J. P. Klein-Szanto1Samuel Litwin2Emmanuelle Nicolas3Raid Al-Jumaily4Peter Alexander5Andrew K. Godwin6Eric A. Ross7Russell J. Schilder8Daniel E. Bassi9Department of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Biomathematics and Biostatistics, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Basic Sciences, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USAOvarian Cancer Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Biomathematics and Biostatistics, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USAOvarian Cancer Program, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USADepartment of Pathology, Fox Chase Cancer Center, Philadelphia, Pennsylvania, USABackground: Proprotein convertases (PCs) are serine proteases that after restricted proteolysis activate many proteins that play a crucial role in cancer such as metalloproteinases, growth factors and growth factor receptors, adhesion molecules, and angiogenic factors. Although the expression of several PCs is increased in many tumors, their expression in primary ovarian tumors has not been studied in detail. We sought to determine if there was an association between the expression of the ubiquitously expressed PCs, furin, PACE-4, PC-5 and PC-7, and ovarian tumor progression. Methods: We assessed their expression by RT-PCR, Real-time PCR, Western blot, and immunohistochemistry using cells derived from normal human ovarian surface epithelium (HOSE) and cancer cell lines as well as ovarian epithelial cancer specimens (45 RT-PCR/Real-time PCR, and 120 archival specimens for Immunohistochemistry). Results: We found that furin expression was restricted to the cancer cell lines. In contrast, PACE-4 and PC-7 showed expression only in normal HOSE cells lines. Furthermore, furin was predominantly expressed in primary tumors from patients who survived for less than five years. The other PCs are either expressed in the group of survivors (PC-7 and PACE4) or expressed in low amounts (PC-5). Conclusions: Our studies point to a clear relationship between furin and ovarian cancer. In addition, these results show that furin exhibits the closest association with ovarian cancer among the ubiquitously expressed PCs, arguing against the redundancy of these proteases. In summary, furin may constitute a marker for ovarian tumor progression and could contribute to predict the outcome of this disease.http://dx.doi.org/10.1155/2007/930321 |
| spellingShingle | Robert E. Page Andrés J. P. Klein-Szanto Samuel Litwin Emmanuelle Nicolas Raid Al-Jumaily Peter Alexander Andrew K. Godwin Eric A. Ross Russell J. Schilder Daniel E. Bassi Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer Cellular Oncology |
| title | Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer |
| title_full | Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer |
| title_fullStr | Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer |
| title_full_unstemmed | Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer |
| title_short | Increased Expression of the Pro-Protein Convertase Furin Predicts Decreased Survival in Ovarian Cancer |
| title_sort | increased expression of the pro protein convertase furin predicts decreased survival in ovarian cancer |
| url | http://dx.doi.org/10.1155/2007/930321 |
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