Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus
Type 2 diabetes mellitus (T2DM) is a severe health problem worldwide, reaching epidemic levels. High susceptibility to infections of T2DM patients indicates dysregulated immune responses to pathogens. However, innate immune responses, including monocyte functions, in T2DM are poorly investigated. Th...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/2157434 |
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| _version_ | 1832556487246872576 |
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| author | Lusine Khondkaryan Sona Margaryan David Poghosyan Gayane Manukyan |
| author_facet | Lusine Khondkaryan Sona Margaryan David Poghosyan Gayane Manukyan |
| author_sort | Lusine Khondkaryan |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) is a severe health problem worldwide, reaching epidemic levels. High susceptibility to infections of T2DM patients indicates dysregulated immune responses to pathogens. However, innate immune responses, including monocyte functions, in T2DM are poorly investigated. Therefore, in this study we aimed to assess lipopolysaccharide- (LPS-) induced immune responses of circulating monocytes from T2DM patients. The results showed that monocytes from T2DM were hyporesponsive to LPS challenge as reflected by significantly suppressed secretion of TNFα (p<0.01) and expression of CD11b (p<0.001) and TLR4 (p<0.001) compared to those in monocytes from healthy subjects. Furthermore, LPS-induced IL-10 levels were similar in diabetic and healthy supernatants, while expression levels of CD163 were found to be downregulated on monocytes from T2DM (p<0.001) suggesting impaired ability of monocytes to switch their phenotype to anti-inflammatory. Taken together, our results suggest compromised function of monocytes in T2DM, which may explain, at least partly, high incidence of infection in these patients. |
| format | Article |
| id | doaj-art-9f9f7cdbce6e407a8b35e1f08ae7c72a |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-9f9f7cdbce6e407a8b35e1f08ae7c72a2025-02-03T05:45:13ZengWileyInternational Journal of Inflammation2090-80402042-00992018-01-01201810.1155/2018/21574342157434Impaired Inflammatory Response to LPS in Type 2 Diabetes MellitusLusine Khondkaryan0Sona Margaryan1David Poghosyan2Gayane Manukyan3Group of Cell Technologies, Institute of Molecular Biology, National Academy of Sciences, Yerevan, ArmeniaLaboratory of Molecular and Cellular Immunology, Institute of Molecular Biology, National Academy of Sciences, Yerevan, ArmeniaLaboratory of Molecular and Cellular Immunology, Institute of Molecular Biology, National Academy of Sciences, Yerevan, ArmeniaLaboratory of Molecular and Cellular Immunology, Institute of Molecular Biology, National Academy of Sciences, Yerevan, ArmeniaType 2 diabetes mellitus (T2DM) is a severe health problem worldwide, reaching epidemic levels. High susceptibility to infections of T2DM patients indicates dysregulated immune responses to pathogens. However, innate immune responses, including monocyte functions, in T2DM are poorly investigated. Therefore, in this study we aimed to assess lipopolysaccharide- (LPS-) induced immune responses of circulating monocytes from T2DM patients. The results showed that monocytes from T2DM were hyporesponsive to LPS challenge as reflected by significantly suppressed secretion of TNFα (p<0.01) and expression of CD11b (p<0.001) and TLR4 (p<0.001) compared to those in monocytes from healthy subjects. Furthermore, LPS-induced IL-10 levels were similar in diabetic and healthy supernatants, while expression levels of CD163 were found to be downregulated on monocytes from T2DM (p<0.001) suggesting impaired ability of monocytes to switch their phenotype to anti-inflammatory. Taken together, our results suggest compromised function of monocytes in T2DM, which may explain, at least partly, high incidence of infection in these patients.http://dx.doi.org/10.1155/2018/2157434 |
| spellingShingle | Lusine Khondkaryan Sona Margaryan David Poghosyan Gayane Manukyan Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus International Journal of Inflammation |
| title | Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus |
| title_full | Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus |
| title_fullStr | Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus |
| title_full_unstemmed | Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus |
| title_short | Impaired Inflammatory Response to LPS in Type 2 Diabetes Mellitus |
| title_sort | impaired inflammatory response to lps in type 2 diabetes mellitus |
| url | http://dx.doi.org/10.1155/2018/2157434 |
| work_keys_str_mv | AT lusinekhondkaryan impairedinflammatoryresponsetolpsintype2diabetesmellitus AT sonamargaryan impairedinflammatoryresponsetolpsintype2diabetesmellitus AT davidpoghosyan impairedinflammatoryresponsetolpsintype2diabetesmellitus AT gayanemanukyan impairedinflammatoryresponsetolpsintype2diabetesmellitus |