Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019
Escherichia coli (E. coli) is a zoonotic pathogen that showed growing resistance to antibiotics. No descriptive analysis highlights the threat of antimicrobial-resistant (AMR) of E. coli among livestock in the United Arab Emirates (UAE). Herein, we conducted phenotypic and genotypic resistance studi...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/3411560 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550157119389696 |
|---|---|
| author | Ghada Elderdiri Abdelwahab Hassan Zackaria Ali Ishag Zulaikha Mohamed Al Hammadi Saeed Mohamed S Al Yammahi Mohd Faoruk Bin Mohd Yusof Muna Sayed Y. Al Yassi Shaikha Saeed A. Al neyadi Asma Mohammed A. Al Mansoori Fawzia Hassan A. Al Hamadi Ibtesam Abdullah S. Al Hamadi Mohamed Ali Abdalla Al Hosani Salama Suhail Mohammed Al Muhairi |
| author_facet | Ghada Elderdiri Abdelwahab Hassan Zackaria Ali Ishag Zulaikha Mohamed Al Hammadi Saeed Mohamed S Al Yammahi Mohd Faoruk Bin Mohd Yusof Muna Sayed Y. Al Yassi Shaikha Saeed A. Al neyadi Asma Mohammed A. Al Mansoori Fawzia Hassan A. Al Hamadi Ibtesam Abdullah S. Al Hamadi Mohamed Ali Abdalla Al Hosani Salama Suhail Mohammed Al Muhairi |
| author_sort | Ghada Elderdiri Abdelwahab |
| collection | DOAJ |
| description | Escherichia coli (E. coli) is a zoonotic pathogen that showed growing resistance to antibiotics. No descriptive analysis highlights the threat of antimicrobial-resistant (AMR) of E. coli among livestock in the United Arab Emirates (UAE). Herein, we conducted phenotypic and genotypic resistance studies on E. coli isolates from livestock samples in the Emirate of Abu Dhabi based on routine diagnosis between the periods 2014–2019. Bacterial culture and disk diffusion methods were used for bacterial isolation and phenotypic resistance analysis. Resistance mechanism was studied by PCR targeting the most commonly resistance genes: ampicillin (blaSHV, blaCMY, and blaTEM-1B), tetracyclines (tetA and tetB), co-trimoxazole [sulfamethoxazole (sul1, sul2, and sul3) + trimethoprim (dfrA1 and dfrA17)], aminoglycosides [aph(3'')-Ia, aph(6)-Id, and aac(3)-IV], and fluoroquinolones (qnrA and aac(6’)-Ib-cr). Analysis of 165 E. coli isolates showed resistant to ampicillin, tetracycline, co-trimoxazole, gentamicin, and enrofloxacin by 157/165 (95.4%), 154/165 (93.6%), 141/165 (86%), 139/165 (85%), and 135/165 (82.7%), respectively. Predominant resistance gene/s detected by PCR were blaCMY (119/160, 72%) and blaTEM-1B (154/160, 96.3%) for ampicillin; tetA (162/164, 98.8%) and tetB (112/164, 68.3%) for tetracyclines; sul2 (156/164, 95%), sul3 (138/164, 84%), and dfra17 (74/164, 44.5%) for co-trimoxazole; aph(3'')-Ia (134/164, 82.1%) and aph(6)-Id (161/164, 98.2%) for aminoglycosides; and aac(6’)-Ib-cr (61/61, 100%) for enrofloxacin. Both phenotypic and genotypic analyses revealed that all E. coli isolates were multidrug-resistant (resistance to 3, 4, and 5 antibiotics classes by 3.6%, 57.6%, and 38.8%, respectively) carrying one or more resistance gene/s for the same antibiotic. PCR profiling confirmed the presence of resistance genes corresponding to their antibiotic profile. Results of the study will highlight the knowledge based on E. coli AMR related to livestock in UAE that may call for interventions. |
| format | Article |
| id | doaj-art-9f948a5067c14d0f9f4287936d130686 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9f948a5067c14d0f9f4287936d1306862025-02-03T06:07:33ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/3411560Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019Ghada Elderdiri Abdelwahab0Hassan Zackaria Ali Ishag1Zulaikha Mohamed Al Hammadi2Saeed Mohamed S Al Yammahi3Mohd Faoruk Bin Mohd Yusof4Muna Sayed Y. Al Yassi5Shaikha Saeed A. Al neyadi6Asma Mohammed A. Al Mansoori7Fawzia Hassan A. Al Hamadi8Ibtesam Abdullah S. Al Hamadi9Mohamed Ali Abdalla Al Hosani10Salama Suhail Mohammed Al Muhairi11Veterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionVeterinary Laboratories DivisionEscherichia coli (E. coli) is a zoonotic pathogen that showed growing resistance to antibiotics. No descriptive analysis highlights the threat of antimicrobial-resistant (AMR) of E. coli among livestock in the United Arab Emirates (UAE). Herein, we conducted phenotypic and genotypic resistance studies on E. coli isolates from livestock samples in the Emirate of Abu Dhabi based on routine diagnosis between the periods 2014–2019. Bacterial culture and disk diffusion methods were used for bacterial isolation and phenotypic resistance analysis. Resistance mechanism was studied by PCR targeting the most commonly resistance genes: ampicillin (blaSHV, blaCMY, and blaTEM-1B), tetracyclines (tetA and tetB), co-trimoxazole [sulfamethoxazole (sul1, sul2, and sul3) + trimethoprim (dfrA1 and dfrA17)], aminoglycosides [aph(3'')-Ia, aph(6)-Id, and aac(3)-IV], and fluoroquinolones (qnrA and aac(6’)-Ib-cr). Analysis of 165 E. coli isolates showed resistant to ampicillin, tetracycline, co-trimoxazole, gentamicin, and enrofloxacin by 157/165 (95.4%), 154/165 (93.6%), 141/165 (86%), 139/165 (85%), and 135/165 (82.7%), respectively. Predominant resistance gene/s detected by PCR were blaCMY (119/160, 72%) and blaTEM-1B (154/160, 96.3%) for ampicillin; tetA (162/164, 98.8%) and tetB (112/164, 68.3%) for tetracyclines; sul2 (156/164, 95%), sul3 (138/164, 84%), and dfra17 (74/164, 44.5%) for co-trimoxazole; aph(3'')-Ia (134/164, 82.1%) and aph(6)-Id (161/164, 98.2%) for aminoglycosides; and aac(6’)-Ib-cr (61/61, 100%) for enrofloxacin. Both phenotypic and genotypic analyses revealed that all E. coli isolates were multidrug-resistant (resistance to 3, 4, and 5 antibiotics classes by 3.6%, 57.6%, and 38.8%, respectively) carrying one or more resistance gene/s for the same antibiotic. PCR profiling confirmed the presence of resistance genes corresponding to their antibiotic profile. Results of the study will highlight the knowledge based on E. coli AMR related to livestock in UAE that may call for interventions.http://dx.doi.org/10.1155/2022/3411560 |
| spellingShingle | Ghada Elderdiri Abdelwahab Hassan Zackaria Ali Ishag Zulaikha Mohamed Al Hammadi Saeed Mohamed S Al Yammahi Mohd Faoruk Bin Mohd Yusof Muna Sayed Y. Al Yassi Shaikha Saeed A. Al neyadi Asma Mohammed A. Al Mansoori Fawzia Hassan A. Al Hamadi Ibtesam Abdullah S. Al Hamadi Mohamed Ali Abdalla Al Hosani Salama Suhail Mohammed Al Muhairi Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 International Journal of Microbiology |
| title | Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 |
| title_full | Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 |
| title_fullStr | Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 |
| title_full_unstemmed | Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 |
| title_short | Antibiotics Resistance in Escherichia coli Isolated from Livestock in the Emirate of Abu Dhabi, UAE, 2014–2019 |
| title_sort | antibiotics resistance in escherichia coli isolated from livestock in the emirate of abu dhabi uae 2014 2019 |
| url | http://dx.doi.org/10.1155/2022/3411560 |
| work_keys_str_mv | AT ghadaelderdiriabdelwahab antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT hassanzackariaaliishag antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT zulaikhamohamedalhammadi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT saeedmohamedsalyammahi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT mohdfaorukbinmohdyusof antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT munasayedyalyassi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT shaikhasaeedaalneyadi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT asmamohammedaalmansoori antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT fawziahassanaalhamadi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT ibtesamabdullahsalhamadi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT mohamedaliabdallaalhosani antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 AT salamasuhailmohammedalmuhairi antibioticsresistanceinescherichiacoliisolatedfromlivestockintheemirateofabudhabiuae20142019 |