Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats
Skin wound healing is a complex physiological process that involves many different cell types and signaling molecules. In traditional Chinese medicine, A. membranaceus and Panax notoginseng are commonly used together for the treatment of wound injury for their significant efficacy. The application o...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1526828/full |
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| author | Jing Li Jing Li Linai Li Yue Yu Ruixin Qin Chaoyan Yu Chen Chen Youming Dong Yadong Tan Yunen Liu Xuanli Liu |
| author_facet | Jing Li Jing Li Linai Li Yue Yu Ruixin Qin Chaoyan Yu Chen Chen Youming Dong Yadong Tan Yunen Liu Xuanli Liu |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Skin wound healing is a complex physiological process that involves many different cell types and signaling molecules. In traditional Chinese medicine, A. membranaceus and Panax notoginseng are commonly used together for the treatment of wound injury for their significant efficacy. The application of new materials may make A. membranaceus-P. notoginseng (AP) play a better curative effect. In this study, we fabricated a Carboxymethyl chitosan-Sodium alginate hydrogel loaded with the extract of Astragalus membranaceus-P. notoginseng (APCS), which showed favorable stability, biocompatibility, and the ability to rapidly release drugs. Cell culture experiments demonstrated that the APCS hydrogel dramatically increased fibroblast proliferation, migration, and differentiation into myofibroblast. In vivo experiments of wound healing on SD rats showed that APCS hydrogels significantly accelerated skin wound healing, reduced inflammatory response, enhanced the formation of blood vessels, granulation tissues, and collagen fibers, and promoted re-epithelialization at the wound site. Increased expression of catalase, VEGF, and PGP9.5 of wound tissue indicated that APCS hydrogels inhibited oxidative stress and promoted vascular and neuronal regeneration. In summary, the APCS hydrogel displayed great potential as a dressing for achieving satisfactory healing of full-thickness wounds. |
| format | Article |
| id | doaj-art-9f8fb6c6f28240ddb91b5d11219e2f94 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-9f8fb6c6f28240ddb91b5d11219e2f942025-01-29T05:21:29ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011610.3389/fphar.2025.15268281526828Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in ratsJing Li0Jing Li1Linai Li2Yue Yu3Ruixin Qin4Chaoyan Yu5Chen Chen6Youming Dong7Yadong Tan8Yunen Liu9Xuanli Liu10Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, ChinaTeaching and Research Section of The Internal Medicine of Traditional Chinese Medicine, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaMaterial & Process Analysis (Technologie Werkstoff - und Verfahrensanalytik), BMW Brilliance Automotive Ltd., Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaShuren International College, Shenyang Medical College, Shenyang, ChinaSkin wound healing is a complex physiological process that involves many different cell types and signaling molecules. In traditional Chinese medicine, A. membranaceus and Panax notoginseng are commonly used together for the treatment of wound injury for their significant efficacy. The application of new materials may make A. membranaceus-P. notoginseng (AP) play a better curative effect. In this study, we fabricated a Carboxymethyl chitosan-Sodium alginate hydrogel loaded with the extract of Astragalus membranaceus-P. notoginseng (APCS), which showed favorable stability, biocompatibility, and the ability to rapidly release drugs. Cell culture experiments demonstrated that the APCS hydrogel dramatically increased fibroblast proliferation, migration, and differentiation into myofibroblast. In vivo experiments of wound healing on SD rats showed that APCS hydrogels significantly accelerated skin wound healing, reduced inflammatory response, enhanced the formation of blood vessels, granulation tissues, and collagen fibers, and promoted re-epithelialization at the wound site. Increased expression of catalase, VEGF, and PGP9.5 of wound tissue indicated that APCS hydrogels inhibited oxidative stress and promoted vascular and neuronal regeneration. In summary, the APCS hydrogel displayed great potential as a dressing for achieving satisfactory healing of full-thickness wounds.https://www.frontiersin.org/articles/10.3389/fphar.2025.1526828/fullCarboxymethyl chitosan-sodium alginate hydrogelastragali membranaceusPanax notoginsengratswound healing |
| spellingShingle | Jing Li Jing Li Linai Li Yue Yu Ruixin Qin Chaoyan Yu Chen Chen Youming Dong Yadong Tan Yunen Liu Xuanli Liu Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats Frontiers in Pharmacology Carboxymethyl chitosan-sodium alginate hydrogel astragali membranaceus Panax notoginseng rats wound healing |
| title | Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats |
| title_full | Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats |
| title_fullStr | Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats |
| title_full_unstemmed | Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats |
| title_short | Effect of Carboxymethyl chitosan-sodium alginate hydrogel loaded with Astragalus membranaceus-Panax notoginseng on wound healing in rats |
| title_sort | effect of carboxymethyl chitosan sodium alginate hydrogel loaded with astragalus membranaceus panax notoginseng on wound healing in rats |
| topic | Carboxymethyl chitosan-sodium alginate hydrogel astragali membranaceus Panax notoginseng rats wound healing |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1526828/full |
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