Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity
Due to the increase in aging populations and the prevalence of aging-associated diseases, there is a growing interest in finding therapeutic interventions. Antioxidants play a vital role in mitigating the adverse conditions associated with aging. In this study, we investigated the impact of Dendrobi...
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KeAi Communications Co. Ltd.
2025-11-01
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| Series: | Journal of Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772566924000946 |
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| author | Yichen Liu Arome Solomon Odiba Qi Yu Jin Xie Nkwachukwu Oziamara Okoro Guiyan Liao Xinchen Zhu Bin He Cheng Jin Wenxia Fang Bin Wang |
| author_facet | Yichen Liu Arome Solomon Odiba Qi Yu Jin Xie Nkwachukwu Oziamara Okoro Guiyan Liao Xinchen Zhu Bin He Cheng Jin Wenxia Fang Bin Wang |
| author_sort | Yichen Liu |
| collection | DOAJ |
| description | Due to the increase in aging populations and the prevalence of aging-associated diseases, there is a growing interest in finding therapeutic interventions. Antioxidants play a vital role in mitigating the adverse conditions associated with aging. In this study, we investigated the impact of Dendrobium officinale alkaloids (DOA) cultivated in diverse environments on antioxidative and anti-aging responses in Caenorhabditis elegans. Three distinct sources of DOA, represented as tree (TR)-DOA, greenhouse (GH)-DOA, and rock (RK)-DOA, were examined. Following initial testing of three DOA concentrations, 10 µg/mL was selected, which increased the TJ1060 lifespan by 23%, and exhibited no toxicity. TR-DOA, GH-DOA, and RK-DOA exhibited robust antioxidative effects, significantly reducing reactive oxygen species levels by 37%, 54%, and 60%, respectively on day 1, and 68%, 73%, and 75% on day 5, respectively. On day 4, TR-DOA, GH-DOA, and RK-DOA significantly reduced lipofuscin levels by 36%, 51%, and 39%, respectively (P < 0.000 1). On day 8, lipofuscin levels were significantly reduced by 34% (P < 0.01), 32% (P < 0.05), and 33% (P < 0.05), respectively. TR-DOA, GH-DOA, and RK-DOA, likewise, reduced the level of the endoplasmic reticulum stress marker, HSP-4::GFP, by 36%, 37%, and 35%, respectively (P < 0.000 1) on day 1, and by 38%, 40%, and 45%, respectively (P < 0.000 1) on day 8. Compared to the control, RK-DOA, GH-DOA, and TR-DOA significantly upregulated HSP-6::GFP (P < 0.0001, P < 0.001, and P < 0.05, respectively), and enhanced heat stress resistance (P < 0.0001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA significantly reduced α-synuclein aggregation by 31%, 25%, and 18% respectively on day 1 (P < 0.0001, P < 0.0001, and P < 0.01, respectively), and by 32%, 27%, and 12% on day 3 (P < 0.01, P < 0.01, and P > 0.05, respectively). RK-DOA, GH-DOA, and TR-DOA delayed paralysis (P < 0.0001, in all cases) and significantly increased worm activity (P < 0.001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA also significantly increased the rate of body bend on day 5 (P < 0.001, P < 0.01, P < 0.0001) and day 10 (P < 0.001, P < 0.0001, P < 0.01). Our findings suggest that DOA, particularly RK-DOA, offers promising antioxidative and anti-aging benefits in C. elegans. The differential responses among DOA variants highlight the importance of the cultivation environment in shaping the bioactivity of natural compounds. |
| format | Article |
| id | doaj-art-9f8d2e8ab6d044efbeac2eab9029691e |
| institution | Kabale University |
| issn | 2772-5669 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Journal of Future Foods |
| spelling | doaj-art-9f8d2e8ab6d044efbeac2eab9029691e2025-01-30T05:15:14ZengKeAi Communications Co. Ltd.Journal of Future Foods2772-56692025-11-0156591604Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activityYichen Liu0Arome Solomon Odiba1Qi Yu2Jin Xie3Nkwachukwu Oziamara Okoro4Guiyan Liao5Xinchen Zhu6Bin He7Cheng Jin8Wenxia Fang9Bin Wang10National Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; State Key Lab of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaSchool of Agriculture and Engineering, Guangxi Vocational and Technical College, Nanning 530226, ChinaState Key Lab of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaNational Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; College of Life Science and Technology, Guangxi University, Nanning 530004, China; Corresponding authors at: National Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China.National Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China; Corresponding authors at: National Key Laboratory of Non-food Biomass Technology, Institute of Biological Sciences and Technology, Guangxi Academy of Sciences, Nanning 530007, China.Due to the increase in aging populations and the prevalence of aging-associated diseases, there is a growing interest in finding therapeutic interventions. Antioxidants play a vital role in mitigating the adverse conditions associated with aging. In this study, we investigated the impact of Dendrobium officinale alkaloids (DOA) cultivated in diverse environments on antioxidative and anti-aging responses in Caenorhabditis elegans. Three distinct sources of DOA, represented as tree (TR)-DOA, greenhouse (GH)-DOA, and rock (RK)-DOA, were examined. Following initial testing of three DOA concentrations, 10 µg/mL was selected, which increased the TJ1060 lifespan by 23%, and exhibited no toxicity. TR-DOA, GH-DOA, and RK-DOA exhibited robust antioxidative effects, significantly reducing reactive oxygen species levels by 37%, 54%, and 60%, respectively on day 1, and 68%, 73%, and 75% on day 5, respectively. On day 4, TR-DOA, GH-DOA, and RK-DOA significantly reduced lipofuscin levels by 36%, 51%, and 39%, respectively (P < 0.000 1). On day 8, lipofuscin levels were significantly reduced by 34% (P < 0.01), 32% (P < 0.05), and 33% (P < 0.05), respectively. TR-DOA, GH-DOA, and RK-DOA, likewise, reduced the level of the endoplasmic reticulum stress marker, HSP-4::GFP, by 36%, 37%, and 35%, respectively (P < 0.000 1) on day 1, and by 38%, 40%, and 45%, respectively (P < 0.000 1) on day 8. Compared to the control, RK-DOA, GH-DOA, and TR-DOA significantly upregulated HSP-6::GFP (P < 0.0001, P < 0.001, and P < 0.05, respectively), and enhanced heat stress resistance (P < 0.0001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA significantly reduced α-synuclein aggregation by 31%, 25%, and 18% respectively on day 1 (P < 0.0001, P < 0.0001, and P < 0.01, respectively), and by 32%, 27%, and 12% on day 3 (P < 0.01, P < 0.01, and P > 0.05, respectively). RK-DOA, GH-DOA, and TR-DOA delayed paralysis (P < 0.0001, in all cases) and significantly increased worm activity (P < 0.001, P < 0.001, and P < 0.01, respectively). RK-DOA, GH-DOA, and TR-DOA also significantly increased the rate of body bend on day 5 (P < 0.001, P < 0.01, P < 0.0001) and day 10 (P < 0.001, P < 0.0001, P < 0.01). Our findings suggest that DOA, particularly RK-DOA, offers promising antioxidative and anti-aging benefits in C. elegans. The differential responses among DOA variants highlight the importance of the cultivation environment in shaping the bioactivity of natural compounds.http://www.sciencedirect.com/science/article/pii/S2772566924000946AlkaloidsAnti-agingAntioxidant, Caenorhabditis elegansDendrobium officinaleEthnopharmacology |
| spellingShingle | Yichen Liu Arome Solomon Odiba Qi Yu Jin Xie Nkwachukwu Oziamara Okoro Guiyan Liao Xinchen Zhu Bin He Cheng Jin Wenxia Fang Bin Wang Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity Journal of Future Foods Alkaloids Anti-aging Antioxidant, Caenorhabditis elegans Dendrobium officinale Ethnopharmacology |
| title | Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| title_full | Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| title_fullStr | Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| title_full_unstemmed | Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| title_short | Alkaloids extracted from Dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| title_sort | alkaloids extracted from dendrobium officinale grown in diverse environments exhibited robust antioxidative and antiaging activity |
| topic | Alkaloids Anti-aging Antioxidant, Caenorhabditis elegans Dendrobium officinale Ethnopharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S2772566924000946 |
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