Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform
Extracellular vesicles (EVs) are a part of a cell-to-cell communication system of prokaryotic and eukaryotic organisms. Their ability to penetrate biological barriers and to transfer molecules between cells shows their potential as a novel class of drug delivery platform. However, because of the gre...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177924001011 |
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| author | Aleksandra Steć Andrea Heinz Szymon Dziomba |
| author_facet | Aleksandra Steć Andrea Heinz Szymon Dziomba |
| author_sort | Aleksandra Steć |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) are a part of a cell-to-cell communication system of prokaryotic and eukaryotic organisms. Their ability to penetrate biological barriers and to transfer molecules between cells shows their potential as a novel class of drug delivery platform. However, because of the great heterogeneity of EVs and the complexity of biological matrices from which they are typically isolated, reliable quality control procedures need to be established to ensure their safety for medical use. According to current recommendations, quantification of EVs, confirmation of their identity, and purity assessment require the use of several analytical techniques, including particle-size distribution analysis, proteomics, and electron microscopy, making the characterization process demanding. Capillary electrophoresis (CE) has recently emerged as an alternative tool for EV characterization. In this study, the available literature on this novel concept for EV characterization was reviewed. Its performance was critically evaluated and compared with currently used methods. The utility of CE in the quality control of EV-based medicines was discussed. |
| format | Article |
| id | doaj-art-9f60cf7ef4264d57a6f001b93f5008d9 |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-9f60cf7ef4264d57a6f001b93f5008d92025-01-30T05:13:58ZengElsevierJournal of Pharmaceutical Analysis2095-17792024-12-011412101004Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platformAleksandra Steć0Andrea Heinz1Szymon Dziomba2Department of Toxicology, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdansk, PolandLEO Foundation Center for Cutaneous Drug Delivery, Department of Pharmacy, University of Copenhagen, 2100 Copenhagen, DenmarkDepartment of Toxicology, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdansk, Poland; Corresponding author.Extracellular vesicles (EVs) are a part of a cell-to-cell communication system of prokaryotic and eukaryotic organisms. Their ability to penetrate biological barriers and to transfer molecules between cells shows their potential as a novel class of drug delivery platform. However, because of the great heterogeneity of EVs and the complexity of biological matrices from which they are typically isolated, reliable quality control procedures need to be established to ensure their safety for medical use. According to current recommendations, quantification of EVs, confirmation of their identity, and purity assessment require the use of several analytical techniques, including particle-size distribution analysis, proteomics, and electron microscopy, making the characterization process demanding. Capillary electrophoresis (CE) has recently emerged as an alternative tool for EV characterization. In this study, the available literature on this novel concept for EV characterization was reviewed. Its performance was critically evaluated and compared with currently used methods. The utility of CE in the quality control of EV-based medicines was discussed.http://www.sciencedirect.com/science/article/pii/S2095177924001011Capillary electrophoresisCharacterizationElectrolyteExtracellular vesiclesHeterogeneity |
| spellingShingle | Aleksandra Steć Andrea Heinz Szymon Dziomba Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform Journal of Pharmaceutical Analysis Capillary electrophoresis Characterization Electrolyte Extracellular vesicles Heterogeneity |
| title | Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform |
| title_full | Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform |
| title_fullStr | Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform |
| title_full_unstemmed | Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform |
| title_short | Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform |
| title_sort | characterization of extracellular vesicles by capillary zone electrophoresis a novel concept for characterization of a next generation drug delivery platform |
| topic | Capillary electrophoresis Characterization Electrolyte Extracellular vesicles Heterogeneity |
| url | http://www.sciencedirect.com/science/article/pii/S2095177924001011 |
| work_keys_str_mv | AT aleksandrastec characterizationofextracellularvesiclesbycapillaryzoneelectrophoresisanovelconceptforcharacterizationofanextgenerationdrugdeliveryplatform AT andreaheinz characterizationofextracellularvesiclesbycapillaryzoneelectrophoresisanovelconceptforcharacterizationofanextgenerationdrugdeliveryplatform AT szymondziomba characterizationofextracellularvesiclesbycapillaryzoneelectrophoresisanovelconceptforcharacterizationofanextgenerationdrugdeliveryplatform |