A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer

MiR-10b, miR-335, and miR-21 are classes of microRNAs (miRNAs) that are overexpressed in breast cancer. Thus, in our study we aimed to test the hypothesis that miRNAs may have direct interactions with proteins and the possibility to inhibit/activate the functional site of proteins and enzymes. For t...

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Main Authors: Rahma Hammou, Yassine Kasmi, Moulay Ennaji
Format: Article
Language:English
Published: Termedia Publishing House 2019-12-01
Series:Contemporary Oncology
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Online Access:https://www.termedia.pl/A-computational-approach-to-the-study-of-interactions-between-proteins-and-miR10-b-miR-335-and-miR-21-involved-in-breast-cancer,3,39557,1,1.html
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author Rahma Hammou
Yassine Kasmi
Moulay Ennaji
author_facet Rahma Hammou
Yassine Kasmi
Moulay Ennaji
author_sort Rahma Hammou
collection DOAJ
description MiR-10b, miR-335, and miR-21 are classes of microRNAs (miRNAs) that are overexpressed in breast cancer. Thus, in our study we aimed to test the hypothesis that miRNAs may have direct interactions with proteins and the possibility to inhibit/activate the functional site of proteins and enzymes. For this purpose, we choose three miRNAs involved in breast cancer to study interactions between some proteins and genes, including BRCA1 and PTEN, by processing the docking and matching tools using the Hex8 and HADDOCK server. Mathematically, the hidden Markov models were created by using MATLAB script according to the algorithm in order to study and validate the interactions and bonds between proteins and miRNAs. The main results demonstrate the ability of miR-10b, miR-335, and miR-21 to create direct interactions with 3D protein structures. Furthermore, these results may lead to another pathway of research, i.e. the direct interaction between proteins and their sub-units, to highlight the data obtained previously and demonstrate that proteins may directly interact with ncRNA instead of mRNA. Moreover, our study suggests developing research on different pathways of association proteins-miRNAs as a part of epigenetic extra-nuclear regulation. Taken together, our study provides the first evidence of direct interactions between miRNAs and proteins.
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spelling doaj-art-9f5323c189fd4fb8995b88ece75a8efc2025-08-20T02:18:39ZengTermedia Publishing HouseContemporary Oncology1428-25261897-43092019-12-0123422022510.5114/wo.2019.9154439557A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancerRahma HammouYassine KasmiMoulay EnnajiMiR-10b, miR-335, and miR-21 are classes of microRNAs (miRNAs) that are overexpressed in breast cancer. Thus, in our study we aimed to test the hypothesis that miRNAs may have direct interactions with proteins and the possibility to inhibit/activate the functional site of proteins and enzymes. For this purpose, we choose three miRNAs involved in breast cancer to study interactions between some proteins and genes, including BRCA1 and PTEN, by processing the docking and matching tools using the Hex8 and HADDOCK server. Mathematically, the hidden Markov models were created by using MATLAB script according to the algorithm in order to study and validate the interactions and bonds between proteins and miRNAs. The main results demonstrate the ability of miR-10b, miR-335, and miR-21 to create direct interactions with 3D protein structures. Furthermore, these results may lead to another pathway of research, i.e. the direct interaction between proteins and their sub-units, to highlight the data obtained previously and demonstrate that proteins may directly interact with ncRNA instead of mRNA. Moreover, our study suggests developing research on different pathways of association proteins-miRNAs as a part of epigenetic extra-nuclear regulation. Taken together, our study provides the first evidence of direct interactions between miRNAs and proteins.https://www.termedia.pl/A-computational-approach-to-the-study-of-interactions-between-proteins-and-miR10-b-miR-335-and-miR-21-involved-in-breast-cancer,3,39557,1,1.htmlmir-10b mir-335 mir-21 breast cancer gene interactions
spellingShingle Rahma Hammou
Yassine Kasmi
Moulay Ennaji
A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
Contemporary Oncology
mir-10b
mir-335
mir-21
breast cancer
gene interactions
title A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
title_full A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
title_fullStr A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
title_full_unstemmed A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
title_short A computational approach to the study of interactions between proteins and miR10-b, miR-335, and miR-21 involved in breast cancer
title_sort computational approach to the study of interactions between proteins and mir10 b mir 335 and mir 21 involved in breast cancer
topic mir-10b
mir-335
mir-21
breast cancer
gene interactions
url https://www.termedia.pl/A-computational-approach-to-the-study-of-interactions-between-proteins-and-miR10-b-miR-335-and-miR-21-involved-in-breast-cancer,3,39557,1,1.html
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