Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway
Background: Oxidative aggression is a hallmark of varicocele and may depend on decreased reactive ability ofthe endogenous antioxidant system following heat stress. We aimed to investigate the underlying mechanisms.Therefore, the expression of the main enzyme proteins involved in the generation of e...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2025-01-01
|
| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | https://www.ijfs.ir/article_715799_388b2f14f3d4b46f5575e31d8713b642.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832595632728047616 |
|---|---|
| author | Azam Shiasi Marziyeh Tavalaee Ramesh Monajemi Dattilo M Mohammad Hossein Nasr-Esfahani |
| author_facet | Azam Shiasi Marziyeh Tavalaee Ramesh Monajemi Dattilo M Mohammad Hossein Nasr-Esfahani |
| author_sort | Azam Shiasi |
| collection | DOAJ |
| description | Background: Oxidative aggression is a hallmark of varicocele and may depend on decreased reactive ability ofthe endogenous antioxidant system following heat stress. We aimed to investigate the underlying mechanisms.Therefore, the expression of the main enzyme proteins involved in the generation of endogenous antioxidant power,cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE), heme oxygenase (HO-1), and also, some ofthe metabolites (methionine, homocysteine, taurine and vitamin B6) reporting on their activity was investigatedusing a surgical varicocele model in rats.Materials and Methods: In this experimental study, thirty male Wistar rats (7-8 weeks old, weighing 180-220 g)were divided into three groups: control, sham, and varicocele. We evaluated sperm parameters and functional tests,as well as the expression of CBS, CSE, and HO-1 proteins using Western blot analysis. Serum levels of methionine,homocysteine, and taurine were analyzed through high-performance liquid chromatography, while vitamin B6concentrations were measured using enzyme-linked immunosorbent assay. For group comparisons, we employedANOVA and the Tukey test, considering a P<0.05 as significant.Results: We observed a significant reduction in both sperm quality and functional parameters. Additionally, therewas a notable decrease in the expression of CBS, CSE, and HO-1 proteins, as well as circulating vitamin B6 levels,in the varicocele group compared to the control and sham groups (P<0.001).Conclusion: The results of this study suggest that one possible cause of increased oxidative stress in varicocelemay be the reduced expression of testicular enzymes involved in the production of endogenous antioxidants, whichare associated with the transsulfuration pathway. However, further studies are needed to confirm these findings. |
| format | Article |
| id | doaj-art-9f4ae9c036a249ab9d40b95781ee3fd8 |
| institution | Kabale University |
| issn | 2008-076X 2008-0778 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj-art-9f4ae9c036a249ab9d40b95781ee3fd82025-01-18T09:23:38ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782025-01-01191808710.22074/ijfs.2024.2041190.1761715799Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration PathwayAzam Shiasi0Marziyeh Tavalaee1Ramesh Monajemi2Dattilo M3Mohammad Hossein Nasr-Esfahani4Department of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranDepartment of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, IranR&D Department, Parthenogen, Piazza Indipendenza 11, Lugano 6900, SwitzerlandDepartment of Reproductive Biotechnology, Reproductive Biomedicine Research Centre, Royan Institute for Biotechnology, ACECR, Isfahan, IranBackground: Oxidative aggression is a hallmark of varicocele and may depend on decreased reactive ability ofthe endogenous antioxidant system following heat stress. We aimed to investigate the underlying mechanisms.Therefore, the expression of the main enzyme proteins involved in the generation of endogenous antioxidant power,cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CSE), heme oxygenase (HO-1), and also, some ofthe metabolites (methionine, homocysteine, taurine and vitamin B6) reporting on their activity was investigatedusing a surgical varicocele model in rats.Materials and Methods: In this experimental study, thirty male Wistar rats (7-8 weeks old, weighing 180-220 g)were divided into three groups: control, sham, and varicocele. We evaluated sperm parameters and functional tests,as well as the expression of CBS, CSE, and HO-1 proteins using Western blot analysis. Serum levels of methionine,homocysteine, and taurine were analyzed through high-performance liquid chromatography, while vitamin B6concentrations were measured using enzyme-linked immunosorbent assay. For group comparisons, we employedANOVA and the Tukey test, considering a P<0.05 as significant.Results: We observed a significant reduction in both sperm quality and functional parameters. Additionally, therewas a notable decrease in the expression of CBS, CSE, and HO-1 proteins, as well as circulating vitamin B6 levels,in the varicocele group compared to the control and sham groups (P<0.001).Conclusion: The results of this study suggest that one possible cause of increased oxidative stress in varicocelemay be the reduced expression of testicular enzymes involved in the production of endogenous antioxidants, whichare associated with the transsulfuration pathway. However, further studies are needed to confirm these findings.https://www.ijfs.ir/article_715799_388b2f14f3d4b46f5575e31d8713b642.pdfcystathionine beta synthasecystathionine gamma lyaseheme oxygenasevaricocelevitamin b6 |
| spellingShingle | Azam Shiasi Marziyeh Tavalaee Ramesh Monajemi Dattilo M Mohammad Hossein Nasr-Esfahani Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway International Journal of Fertility and Sterility cystathionine beta synthase cystathionine gamma lyase heme oxygenase varicocele vitamin b6 |
| title | Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway |
| title_full | Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway |
| title_fullStr | Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway |
| title_full_unstemmed | Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway |
| title_short | Impact of Varicocele on The Expression of Testicular Enzymes Involved in The Transsulfuration Pathway |
| title_sort | impact of varicocele on the expression of testicular enzymes involved in the transsulfuration pathway |
| topic | cystathionine beta synthase cystathionine gamma lyase heme oxygenase varicocele vitamin b6 |
| url | https://www.ijfs.ir/article_715799_388b2f14f3d4b46f5575e31d8713b642.pdf |
| work_keys_str_mv | AT azamshiasi impactofvaricoceleontheexpressionoftesticularenzymesinvolvedinthetranssulfurationpathway AT marziyehtavalaee impactofvaricoceleontheexpressionoftesticularenzymesinvolvedinthetranssulfurationpathway AT rameshmonajemi impactofvaricoceleontheexpressionoftesticularenzymesinvolvedinthetranssulfurationpathway AT dattilom impactofvaricoceleontheexpressionoftesticularenzymesinvolvedinthetranssulfurationpathway AT mohammadhosseinnasresfahani impactofvaricoceleontheexpressionoftesticularenzymesinvolvedinthetranssulfurationpathway |