Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion
Abstract A proof‐of‐principle device for axial high‐resolution operation that combines a deoxyribonucleic acid (DNA) origami with a functionalized graphene layer is presented, analyzed by nanoscopy. Along the DNA origami structure, ATTO‐488 fluorophores are bound at specific distances from graphene,...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-04-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400617 |
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| author | João D. G. Azevedo Tiago Queirós Filipe Camarneiro Maria João Lopes João Freitas Agnes Purwidyantri Praneetha Sundar Prakash Soumya Chandrasekhar Thorsten‐Lars Schmidt Pedro Alpuim Jana B. Nieder |
| author_facet | João D. G. Azevedo Tiago Queirós Filipe Camarneiro Maria João Lopes João Freitas Agnes Purwidyantri Praneetha Sundar Prakash Soumya Chandrasekhar Thorsten‐Lars Schmidt Pedro Alpuim Jana B. Nieder |
| author_sort | João D. G. Azevedo |
| collection | DOAJ |
| description | Abstract A proof‐of‐principle device for axial high‐resolution operation that combines a deoxyribonucleic acid (DNA) origami with a functionalized graphene layer is presented, analyzed by nanoscopy. Along the DNA origami structure, ATTO‐488 fluorophores are bound at specific distances from graphene, from where specific fluorescence lifetime values are expected due to nearfield energy transfer processes. These are characterized by Fluorescence Lifetime Imaging Microscopy (FLIM). Through modulation of the electrostatic potential of graphene under electrical gating, changes in the fluorescence lifetimes are observed. These are understood as the result of changed energy transfer coupling conditions between the fluorophore and graphene's electronic states, combined with a vertical displacement of the DNA origami structure that matches molecular dimensions. A hybrid architecture is provided whose nanoscale operation depends on the applied voltage regime. A potential application of these findings may be envisioned for biocompatible sensing approaches, in medical or environmental sensing. |
| format | Article |
| id | doaj-art-9f3d6670c59a40209e4f6bbe0b62700d |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-9f3d6670c59a40209e4f6bbe0b62700d2025-08-20T02:27:18ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-04-01128n/an/a10.1002/admi.202400617Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale MotionJoão D. G. Azevedo0Tiago Queirós1Filipe Camarneiro2Maria João Lopes3João Freitas4Agnes Purwidyantri5Praneetha Sundar Prakash6Soumya Chandrasekhar7Thorsten‐Lars Schmidt8Pedro Alpuim9Jana B. Nieder10INL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalPhysics Department Kent State University 103 Smith Hall Kent OH 44242 USAPhysics Department Kent State University 103 Smith Hall Kent OH 44242 USAPhysics Department Kent State University 103 Smith Hall Kent OH 44242 USAINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalINL – International Iberian Nanotechnology Laboratory Av. Mestre José Veiga Braga 4715‐330 PortugalAbstract A proof‐of‐principle device for axial high‐resolution operation that combines a deoxyribonucleic acid (DNA) origami with a functionalized graphene layer is presented, analyzed by nanoscopy. Along the DNA origami structure, ATTO‐488 fluorophores are bound at specific distances from graphene, from where specific fluorescence lifetime values are expected due to nearfield energy transfer processes. These are characterized by Fluorescence Lifetime Imaging Microscopy (FLIM). Through modulation of the electrostatic potential of graphene under electrical gating, changes in the fluorescence lifetimes are observed. These are understood as the result of changed energy transfer coupling conditions between the fluorophore and graphene's electronic states, combined with a vertical displacement of the DNA origami structure that matches molecular dimensions. A hybrid architecture is provided whose nanoscale operation depends on the applied voltage regime. A potential application of these findings may be envisioned for biocompatible sensing approaches, in medical or environmental sensing.https://doi.org/10.1002/admi.202400617biosensorsDNA origamifluorescence quenchinggraphenenearfield sensing |
| spellingShingle | João D. G. Azevedo Tiago Queirós Filipe Camarneiro Maria João Lopes João Freitas Agnes Purwidyantri Praneetha Sundar Prakash Soumya Chandrasekhar Thorsten‐Lars Schmidt Pedro Alpuim Jana B. Nieder Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion Advanced Materials Interfaces biosensors DNA origami fluorescence quenching graphene nearfield sensing |
| title | Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion |
| title_full | Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion |
| title_fullStr | Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion |
| title_full_unstemmed | Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion |
| title_short | Hybrid DNA Origami – Graphene Platform for Electrically‐Gated Nanoscale Motion |
| title_sort | hybrid dna origami graphene platform for electrically gated nanoscale motion |
| topic | biosensors DNA origami fluorescence quenching graphene nearfield sensing |
| url | https://doi.org/10.1002/admi.202400617 |
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