The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection
Functional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/389520 |
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| author | Vinnitsa Buzoianu-Anguiano Sandra Orozco-Suárez Elisa García-Vences Sara Caballero-Chacón Gabriel Guizar-Sahagún Luis Chavez-Sanchez Israel Grijalva |
| author_facet | Vinnitsa Buzoianu-Anguiano Sandra Orozco-Suárez Elisa García-Vences Sara Caballero-Chacón Gabriel Guizar-Sahagún Luis Chavez-Sanchez Israel Grijalva |
| author_sort | Vinnitsa Buzoianu-Anguiano |
| collection | DOAJ |
| description | Functional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells (BMSCs) have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of the combined transplantation of PPN and BMSC on regrowth, remyelination, and locomotor function in an adult rat model of spinal cord (SC) transection, 39 Fischer 344 rats underwent SC transection at T9 level. Four weeks later they were randomly assigned to traumatic spinal cord injury (TSCI) without treatment, TSCI + Fibrin Glue (FG), TSCI + FG + PPN, and TSCI + FG + PPN + BMSCs. Eight weeks after, transplantation was carried out on immunofluorescence and electron microscope studies. The results showed greater axonal regrowth and remyelination in experimental groups TSCI + FG + PPN and TSCI + FG + PPN + BMSCs analyzed with GAP-43, neuritin, and myelin basic protein. It is concluded that the combined treatment of PPN and BMSCs is a favorable strategy for axonal regrowth and remyelination in a chronic SC transection model. |
| format | Article |
| id | doaj-art-9f04fe9b59c84df58303addbe9750eb0 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-9f04fe9b59c84df58303addbe9750eb02025-02-03T01:09:45ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/389520389520The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord TransectionVinnitsa Buzoianu-Anguiano0Sandra Orozco-Suárez1Elisa García-Vences2Sara Caballero-Chacón3Gabriel Guizar-Sahagún4Luis Chavez-Sanchez5Israel Grijalva6Medical Research Unit for Neurological Diseases, High Specialty Medical Unit Specialty Hospital, Centro Médico Nacional Siglo XXI, IMSS (Mexican Social Security Institute), Avenida Cuauhtémoc 330, Doctores, Cuauhtémoc, 06720 México, DF, MexicoMedical Research Unit for Neurological Diseases, High Specialty Medical Unit Specialty Hospital, Centro Médico Nacional Siglo XXI, IMSS (Mexican Social Security Institute), Avenida Cuauhtémoc 330, Doctores, Cuauhtémoc, 06720 México, DF, MexicoScientific Unit, Faculty of Medicine, UNAM-INMEGEN (National Autonomous University of Mexico-National Institute of Genomic Medicine), Periférico Sur 4809, Arenal Tepepan, Tlalpan, 14610 México, DF, MexicoDepartment of Physiology and Pharmacy, Faculty of Veterinary Medicine and Zootechnics, UNAM (National Autonomous University of Mexico), Circuito Exterior, Ciudad Universitaria, Coyoacán, 04510 México, DF, MexicoMedical Research Unit for Neurological Diseases, High Specialty Medical Unit Specialty Hospital, Centro Médico Nacional Siglo XXI, IMSS (Mexican Social Security Institute), Avenida Cuauhtémoc 330, Doctores, Cuauhtémoc, 06720 México, DF, MexicoMedical Research Unit for Immunology, High Specialty Medical Unit Pediatric Hospital, Centro Medico Nacional Siglo XXI, IMSS (Mexican Social Security Institute), Avenida Cuauhtémoc 330, Doctores, Cuauhtémoc, 06720 México, DF, MexicoMedical Research Unit for Neurological Diseases, High Specialty Medical Unit Specialty Hospital, Centro Médico Nacional Siglo XXI, IMSS (Mexican Social Security Institute), Avenida Cuauhtémoc 330, Doctores, Cuauhtémoc, 06720 México, DF, MexicoFunctional recovery following spinal cord injury (SCI) is limited by poor axonal and cellular regeneration as well as the failure to replace damaged myelin. Employed separately, both the transplantation of the predegenerated peripheral nerve (PPN) and the transplantation of bone marrow stromal cells (BMSCs) have been shown to promote the regrowth and remyelination of the damaged central axons in SCI models of hemisection, transection, and contusion injury. With the aim to test the effects of the combined transplantation of PPN and BMSC on regrowth, remyelination, and locomotor function in an adult rat model of spinal cord (SC) transection, 39 Fischer 344 rats underwent SC transection at T9 level. Four weeks later they were randomly assigned to traumatic spinal cord injury (TSCI) without treatment, TSCI + Fibrin Glue (FG), TSCI + FG + PPN, and TSCI + FG + PPN + BMSCs. Eight weeks after, transplantation was carried out on immunofluorescence and electron microscope studies. The results showed greater axonal regrowth and remyelination in experimental groups TSCI + FG + PPN and TSCI + FG + PPN + BMSCs analyzed with GAP-43, neuritin, and myelin basic protein. It is concluded that the combined treatment of PPN and BMSCs is a favorable strategy for axonal regrowth and remyelination in a chronic SC transection model.http://dx.doi.org/10.1155/2015/389520 |
| spellingShingle | Vinnitsa Buzoianu-Anguiano Sandra Orozco-Suárez Elisa García-Vences Sara Caballero-Chacón Gabriel Guizar-Sahagún Luis Chavez-Sanchez Israel Grijalva The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection Neural Plasticity |
| title | The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection |
| title_full | The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection |
| title_fullStr | The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection |
| title_full_unstemmed | The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection |
| title_short | The Morphofunctional Effect of the Transplantation of Bone Marrow Stromal Cells and Predegenerated Peripheral Nerve in Chronic Paraplegic Rat Model via Spinal Cord Transection |
| title_sort | morphofunctional effect of the transplantation of bone marrow stromal cells and predegenerated peripheral nerve in chronic paraplegic rat model via spinal cord transection |
| url | http://dx.doi.org/10.1155/2015/389520 |
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