Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia?
The physiological course of pregnancy is closely related to adequate development of the placenta. Shallow invasion of trophoblast as well as decreased development of the placental vascular network are both common features of preeclampsia. To better understand the proangiogenic features of mast cells...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/307189 |
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| author | Grzegorz Szewczyk Michał Pyzlak Jakub Klimkiewicz Wacław Śmiertka Magdalena Miedzińska-Maciejewska Dariusz Szukiewicz |
| author_facet | Grzegorz Szewczyk Michał Pyzlak Jakub Klimkiewicz Wacław Śmiertka Magdalena Miedzińska-Maciejewska Dariusz Szukiewicz |
| author_sort | Grzegorz Szewczyk |
| collection | DOAJ |
| description | The physiological course of pregnancy is closely related to adequate development of the placenta. Shallow invasion of trophoblast as well as decreased development of the placental vascular network are both common features of preeclampsia. To better understand the proangiogenic features of mast cells, in this study we aim to identify the potential relationship between the distribution of mast cells within the placenta and vascular network development. Material and Methods. Placentas from preeclampsia-complicated pregnancies (𝑛=11) and from physiological pregnancies (𝑛=11) were acquired after cesarean section. The concentration of histamine was measured, and immunohistochemical staining for mast cell tryptase was performed. Morphometric analysis was then performed. Results. We noticed significant differences between the examined groups. Notably, in the preeclampsia group compared to the control group, we observed a higher mean histamine concentration, higher mast cell density (MCD), lower mean mast cell (MMCA) and lower vascular/extravascular (V/EVT) index. In physiological pregnancies, a positive correlation was observed between the histamine concentration and V/VEVT index as well as MCD and the V/VEVT index. In contrast, a negative correlation was observed between MMCA and the V/EVT index in physiological pregnancies. Conclusions. Based on the data from our study, we suggest that a differential distribution of mast cells and corresponding changes in the concentration of histamine are involved in the defective placental vascularization seen in preeclamptic placentas. |
| format | Article |
| id | doaj-art-9f0208560f5d4db0b54452116ee472a0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9f0208560f5d4db0b54452116ee472a02025-02-03T06:01:35ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/307189307189Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia?Grzegorz Szewczyk0Michał Pyzlak1Jakub Klimkiewicz2Wacław Śmiertka3Magdalena Miedzińska-Maciejewska4Dariusz Szukiewicz5Chair and Department of General and Experimental Pathology, Medical University of Warsaw, Ul. Krakowskie Przedmiescie 26/28, 00-927 Warsaw, PolandChair and Department of General and Experimental Pathology, Medical University of Warsaw, Ul. Krakowskie Przedmiescie 26/28, 00-927 Warsaw, PolandDepartment of Anesthesiology and Intensive Care, Military Institute of Health, Ul. Szaserów 128, 04-141 Warsaw, PolandDepartment of Gynecological Oncology, National Institute of Oncology, Ul. Wawelska 15, 02-000 Warsaw, PolandDepartment of Gynecological Oncology, National Institute of Oncology, Ul. Wawelska 15, 02-000 Warsaw, PolandChair and Department of General and Experimental Pathology, Medical University of Warsaw, Ul. Krakowskie Przedmiescie 26/28, 00-927 Warsaw, PolandThe physiological course of pregnancy is closely related to adequate development of the placenta. Shallow invasion of trophoblast as well as decreased development of the placental vascular network are both common features of preeclampsia. To better understand the proangiogenic features of mast cells, in this study we aim to identify the potential relationship between the distribution of mast cells within the placenta and vascular network development. Material and Methods. Placentas from preeclampsia-complicated pregnancies (𝑛=11) and from physiological pregnancies (𝑛=11) were acquired after cesarean section. The concentration of histamine was measured, and immunohistochemical staining for mast cell tryptase was performed. Morphometric analysis was then performed. Results. We noticed significant differences between the examined groups. Notably, in the preeclampsia group compared to the control group, we observed a higher mean histamine concentration, higher mast cell density (MCD), lower mean mast cell (MMCA) and lower vascular/extravascular (V/EVT) index. In physiological pregnancies, a positive correlation was observed between the histamine concentration and V/VEVT index as well as MCD and the V/VEVT index. In contrast, a negative correlation was observed between MMCA and the V/EVT index in physiological pregnancies. Conclusions. Based on the data from our study, we suggest that a differential distribution of mast cells and corresponding changes in the concentration of histamine are involved in the defective placental vascularization seen in preeclamptic placentas.http://dx.doi.org/10.1155/2012/307189 |
| spellingShingle | Grzegorz Szewczyk Michał Pyzlak Jakub Klimkiewicz Wacław Śmiertka Magdalena Miedzińska-Maciejewska Dariusz Szukiewicz Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? Mediators of Inflammation |
| title | Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? |
| title_full | Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? |
| title_fullStr | Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? |
| title_full_unstemmed | Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? |
| title_short | Mast Cells and Histamine: Do They Influence Placental Vascular Network and Development in Preeclampsia? |
| title_sort | mast cells and histamine do they influence placental vascular network and development in preeclampsia |
| url | http://dx.doi.org/10.1155/2012/307189 |
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