Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology
Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by em...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/819067 |
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| author | Bowen Chu Yao Shi Zhimin Li Hao Tian Wanyi Li Yuanzhong Wang |
| author_facet | Bowen Chu Yao Shi Zhimin Li Hao Tian Wanyi Li Yuanzhong Wang |
| author_sort | Bowen Chu |
| collection | DOAJ |
| description | Gentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.61±0.61). |
| format | Article |
| id | doaj-art-9efee3058df1489891957d431a54a253 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-9efee3058df1489891957d431a54a2532025-02-03T00:59:14ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/819067819067Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface MethodologyBowen Chu0Yao Shi1Zhimin Li2Hao Tian3Wanyi Li4Yuanzhong Wang5Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaInstitute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, ChinaGentisides are a class of chemical compounds which is considered as potential therapeutic substance for treatment of neurodegenerative disorders. The heat reflux extraction conditions were optimized for seven kinds of gentisides from the root and rhizome of Gentiana rigescens Franch. ex Hemsl. by employing response surface method. Based on univariate test, a Box-Behnken design (BBD) was applied to the survey of relationships between response value (gentisides yield) and independent variables which were chosen from various extraction processes, including extraction temperature, extraction time, and solvent-material ratio. The optimized conditions for this extraction are as follows: extraction time of 3.40 h, extraction temperature of 74.33°C, and ratio of solvent to raw material of 10.21 : 1 mL/g. Verification assay revealed that the predicted value (99.24%) of extraction parameters from this model was mainly conformed to the experimentally observed values (98.61±0.61).http://dx.doi.org/10.1155/2015/819067 |
| spellingShingle | Bowen Chu Yao Shi Zhimin Li Hao Tian Wanyi Li Yuanzhong Wang Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology Journal of Analytical Methods in Chemistry |
| title | Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology |
| title_full | Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology |
| title_fullStr | Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology |
| title_full_unstemmed | Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology |
| title_short | Optimization of Gentisides Extraction from Gentiana rigescens Franch. ex Hemsl. by Response Surface Methodology |
| title_sort | optimization of gentisides extraction from gentiana rigescens franch ex hemsl by response surface methodology |
| url | http://dx.doi.org/10.1155/2015/819067 |
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