Genetic analysis of weak expression of ABO blood group antigens in neonates
[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed b...
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Institute of Blood Transfusion of Chinese Academy of Medical Sciences
2025-01-01
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| Series: | Zhongguo shuxue zazhi |
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| Online Access: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.014&lang=en |
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| _version_ | 1832585144831049728 |
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| author | YANG Jiali ZHAO Ding LI Wei ZHANG Xiaopan LI Zhihao TIAN Dongdong |
| author_facet | YANG Jiali ZHAO Ding LI Wei ZHANG Xiaopan LI Zhihao TIAN Dongdong |
| author_sort | YANG Jiali |
| collection | DOAJ |
| description | [Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed by tube method and microcolumn gel method. The ABO exons 2-7 were amplified by PCR, and the amplified products were sequenced by Sanger sequencing method to determine the genotype. [Results] Among the ABO blood group serological results of 14 neonates, 8 cases showed weakened A antigen, and 6 cases showed weakened B antigen. Seven samples were identified with ABO subtype alleles, with genotypes as A102/B101+c.538C>T, Aw26/B102, A205/O02, A205/B101(2 cases), Aw26/O02, B(A)06/O01, B101/O01(3 cases), A102/O01(2 cases), A102/B101 and B101/O02. Additionally, three other family members were also found to carry B(A)06 allele in a pedigree investigation. [Conclusion] For samples showing weakened antigens in ABO blood type identification of neonates, it is necessary to consider the possibility of ABO subtype in addition to age factors, and genetic testing can be used to prevent missed detection of ABO subtypes in neonates. |
| format | Article |
| id | doaj-art-9eea6ad76c0742c4b167498219a91323 |
| institution | Kabale University |
| issn | 1004-549X |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Institute of Blood Transfusion of Chinese Academy of Medical Sciences |
| record_format | Article |
| series | Zhongguo shuxue zazhi |
| spelling | doaj-art-9eea6ad76c0742c4b167498219a913232025-01-27T01:46:11ZzhoInstitute of Blood Transfusion of Chinese Academy of Medical SciencesZhongguo shuxue zazhi1004-549X2025-01-01381859010.13303/j.cjbt.issn.1004-549x.2025.01.0141004-549X(2025)1-0085-07Genetic analysis of weak expression of ABO blood group antigens in neonatesYANG Jiali0ZHAO Ding1LI Wei2ZHANG Xiaopan3LI Zhihao4TIAN Dongdong5Department of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, ChinaDepartment of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, ChinaDepartment of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, ChinaDepartment of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, ChinaDepartment of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, ChinaDepartment of Blood Transfusion, Children's Hospital Affiliated to Zhengzhou University, Henan Children's Hospital Zhengzhou Children's Hospital, Zhengzhou 450018, China[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. [Methods] Serological identification of ABO blood group was performed by tube method and microcolumn gel method. The ABO exons 2-7 were amplified by PCR, and the amplified products were sequenced by Sanger sequencing method to determine the genotype. [Results] Among the ABO blood group serological results of 14 neonates, 8 cases showed weakened A antigen, and 6 cases showed weakened B antigen. Seven samples were identified with ABO subtype alleles, with genotypes as A102/B101+c.538C>T, Aw26/B102, A205/O02, A205/B101(2 cases), Aw26/O02, B(A)06/O01, B101/O01(3 cases), A102/O01(2 cases), A102/B101 and B101/O02. Additionally, three other family members were also found to carry B(A)06 allele in a pedigree investigation. [Conclusion] For samples showing weakened antigens in ABO blood type identification of neonates, it is necessary to consider the possibility of ABO subtype in addition to age factors, and genetic testing can be used to prevent missed detection of ABO subtypes in neonates.https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.014&lang=enneonateabo blood groupweak expression of antigenabo subtypegene sequencing |
| spellingShingle | YANG Jiali ZHAO Ding LI Wei ZHANG Xiaopan LI Zhihao TIAN Dongdong Genetic analysis of weak expression of ABO blood group antigens in neonates Zhongguo shuxue zazhi neonate abo blood group weak expression of antigen abo subtype gene sequencing |
| title | Genetic analysis of weak expression of ABO blood group antigens in neonates |
| title_full | Genetic analysis of weak expression of ABO blood group antigens in neonates |
| title_fullStr | Genetic analysis of weak expression of ABO blood group antigens in neonates |
| title_full_unstemmed | Genetic analysis of weak expression of ABO blood group antigens in neonates |
| title_short | Genetic analysis of weak expression of ABO blood group antigens in neonates |
| title_sort | genetic analysis of weak expression of abo blood group antigens in neonates |
| topic | neonate abo blood group weak expression of antigen abo subtype gene sequencing |
| url | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2025.01.014&lang=en |
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