Doubled haploid production in Brassica L.
Doubled haploids (DHs) production through androgenesis is a biotechnological method for genetic improvement of crops. Biotechnological DH line production offers advantages to plant breeders, including the possibility to obtain homozygous lines within a year in contrast to common inbreeding methods,...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/353 |
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| author | N. A. Shmykova D. V. Shumilina T. P. Suprunova |
| author_facet | N. A. Shmykova D. V. Shumilina T. P. Suprunova |
| author_sort | N. A. Shmykova |
| collection | DOAJ |
| description | Doubled haploids (DHs) production through androgenesis is a biotechnological method for genetic improvement of crops. Biotechnological DH line production offers advantages to plant breeders, including the possibility to obtain homozygous lines within a year in contrast to common inbreeding methods, which may take 6–12 years. The greatest success in androgenesis has been achieved in some varieties of rapeseed (Brassica napus L.). However, the efficiency of androgenesis in other Brassica species is still poor. Induction of microspore embryogenesis is usually induced by many factors such as conditions of donor plant growth, genotype, microspore developmental stage, culture medium composition, and culture conditions. The reprogramming of microspores from the gametophytic to the sporophytic habit of development depends on various stress factors. Certain pretreatments of microspores, such as high temperature and colchicine, can favor androgenesis in Brassica species. Plant regeneration from microspores can be improved by proper application of different growth regulators (ethylene, abscisic acid, and indole acetic acid). Optimal combinations of these factors are mandatory for efficient androgenesis. In this review, we summarize the experience of our colleagues in DH-technology in the Brassica genus. Attention is focused on some factors influencing the development of doubled haploid plants and their impact on enhancing the efficiency of androgenesis in Brassica species. |
| format | Article |
| id | doaj-art-9ee7992148044de99c760fa9a65dddbc |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-9ee7992148044de99c760fa9a65dddbc2025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-07-0119111112010.18699/VJ15.014332Doubled haploid production in Brassica L.N. A. Shmykova0D. V. Shumilina1T. P. Suprunova2All-Russia Research Institute of Vegetable Breeding and Seed Production, VNIISSOK village, Odintsovo district, Moscow region, RussiaAll-Russia Research Institute of Vegetable Breeding and Seed Production, VNIISSOK village, Odintsovo district, Moscow region, RussiaAll-Russia Research Institute of Vegetable Breeding and Seed Production, VNIISSOK village, Odintsovo district, Moscow region, RussiaDoubled haploids (DHs) production through androgenesis is a biotechnological method for genetic improvement of crops. Biotechnological DH line production offers advantages to plant breeders, including the possibility to obtain homozygous lines within a year in contrast to common inbreeding methods, which may take 6–12 years. The greatest success in androgenesis has been achieved in some varieties of rapeseed (Brassica napus L.). However, the efficiency of androgenesis in other Brassica species is still poor. Induction of microspore embryogenesis is usually induced by many factors such as conditions of donor plant growth, genotype, microspore developmental stage, culture medium composition, and culture conditions. The reprogramming of microspores from the gametophytic to the sporophytic habit of development depends on various stress factors. Certain pretreatments of microspores, such as high temperature and colchicine, can favor androgenesis in Brassica species. Plant regeneration from microspores can be improved by proper application of different growth regulators (ethylene, abscisic acid, and indole acetic acid). Optimal combinations of these factors are mandatory for efficient androgenesis. In this review, we summarize the experience of our colleagues in DH-technology in the Brassica genus. Attention is focused on some factors influencing the development of doubled haploid plants and their impact on enhancing the efficiency of androgenesis in Brassica species.https://vavilov.elpub.ru/jour/article/view/353brassicadh technologymicrospore cultureembryogenesisdoubled haploids |
| spellingShingle | N. A. Shmykova D. V. Shumilina T. P. Suprunova Doubled haploid production in Brassica L. Вавиловский журнал генетики и селекции brassica dh technology microspore culture embryogenesis doubled haploids |
| title | Doubled haploid production in Brassica L. |
| title_full | Doubled haploid production in Brassica L. |
| title_fullStr | Doubled haploid production in Brassica L. |
| title_full_unstemmed | Doubled haploid production in Brassica L. |
| title_short | Doubled haploid production in Brassica L. |
| title_sort | doubled haploid production in brassica l |
| topic | brassica dh technology microspore culture embryogenesis doubled haploids |
| url | https://vavilov.elpub.ru/jour/article/view/353 |
| work_keys_str_mv | AT nashmykova doubledhaploidproductioninbrassical AT dvshumilina doubledhaploidproductioninbrassical AT tpsuprunova doubledhaploidproductioninbrassical |