Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats
Although referred pain or hypersensitivity has been repeatedly reported in irritable bowel syndrome (IBS) patients and experimental colitis rodents, little is known about the neural mechanisms. Spinal long-term potentiation (LTP) of nociceptive synaptic transmission plays a critical role in the deve...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2019/2098083 |
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| author | Pei-Ran Lv Yang-Shuai Su Wei He Xiao-Yu Wang Hong Shi Xiao-Ning Zhang Bing Zhu Yu Kan Li-Zhen Chen Qiao-Feng Wu Shu-Guang Yu Xiang-Hong Jing |
| author_facet | Pei-Ran Lv Yang-Shuai Su Wei He Xiao-Yu Wang Hong Shi Xiao-Ning Zhang Bing Zhu Yu Kan Li-Zhen Chen Qiao-Feng Wu Shu-Guang Yu Xiang-Hong Jing |
| author_sort | Pei-Ran Lv |
| collection | DOAJ |
| description | Although referred pain or hypersensitivity has been repeatedly reported in irritable bowel syndrome (IBS) patients and experimental colitis rodents, little is known about the neural mechanisms. Spinal long-term potentiation (LTP) of nociceptive synaptic transmission plays a critical role in the development of somatic hyperalgesia in chronic pain conditions. Herein, we sought to determine whether spinal LTP contributes to the referral hyperalgesia in colitis rats and particularly whether electroacupuncture (EA) is effective to alleviate somatic hyperalgesia via suppressing spinal LTP. Rats in the colitis group (induced by colonic infusion of 2,4,6-trinitrobenzenesulfonic acid, TNBS), instead of the control and vehicle groups, displayed evident focal inflammatory destruction of the distal colon accompanied not only with the sensitized visceromotor response (VMR) to noxious colorectal distension (CRD) but also with referral hindpaw hyperalgesia indicated by reduced mechanical and thermal withdrawal latencies. EA at Zusanli (ST36) and Shangjuxu (ST37) attenuated the severity of colonic inflammation, as well as the visceral hypersensitivity and referral hindpaw hyperalgesia in colitis rats. Intriguingly, the threshold of C-fiber-evoked field potentials (CFEFP) was significantly reduced and the spinal LTP was exaggerated in the colitis group, both of which were restored by EA treatment. Taken together, visceral hypersensitivity and referral hindpaw hyperalgesia coexist in TNBS-induced colitis rats, which might be attributed to the enhanced LTP of nociceptive synaptic transmission in the spinal dorsal horn. EA at ST36 and ST37 could relieve visceral hypersensitivity and, in particular, attenuate referral hindpaw hyperalgesia by suppressing the enhanced spinal LTP. |
| format | Article |
| id | doaj-art-9ecf75066292420b80516b5339df68c2 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-9ecf75066292420b80516b5339df68c22025-02-03T01:30:56ZengWileyNeural Plasticity2090-59041687-54432019-01-01201910.1155/2019/20980832098083Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis RatsPei-Ran Lv0Yang-Shuai Su1Wei He2Xiao-Yu Wang3Hong Shi4Xiao-Ning Zhang5Bing Zhu6Yu Kan7Li-Zhen Chen8Qiao-Feng Wu9Shu-Guang Yu10Xiang-Hong Jing11Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaChengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaChengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaInstitute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaAlthough referred pain or hypersensitivity has been repeatedly reported in irritable bowel syndrome (IBS) patients and experimental colitis rodents, little is known about the neural mechanisms. Spinal long-term potentiation (LTP) of nociceptive synaptic transmission plays a critical role in the development of somatic hyperalgesia in chronic pain conditions. Herein, we sought to determine whether spinal LTP contributes to the referral hyperalgesia in colitis rats and particularly whether electroacupuncture (EA) is effective to alleviate somatic hyperalgesia via suppressing spinal LTP. Rats in the colitis group (induced by colonic infusion of 2,4,6-trinitrobenzenesulfonic acid, TNBS), instead of the control and vehicle groups, displayed evident focal inflammatory destruction of the distal colon accompanied not only with the sensitized visceromotor response (VMR) to noxious colorectal distension (CRD) but also with referral hindpaw hyperalgesia indicated by reduced mechanical and thermal withdrawal latencies. EA at Zusanli (ST36) and Shangjuxu (ST37) attenuated the severity of colonic inflammation, as well as the visceral hypersensitivity and referral hindpaw hyperalgesia in colitis rats. Intriguingly, the threshold of C-fiber-evoked field potentials (CFEFP) was significantly reduced and the spinal LTP was exaggerated in the colitis group, both of which were restored by EA treatment. Taken together, visceral hypersensitivity and referral hindpaw hyperalgesia coexist in TNBS-induced colitis rats, which might be attributed to the enhanced LTP of nociceptive synaptic transmission in the spinal dorsal horn. EA at ST36 and ST37 could relieve visceral hypersensitivity and, in particular, attenuate referral hindpaw hyperalgesia by suppressing the enhanced spinal LTP.http://dx.doi.org/10.1155/2019/2098083 |
| spellingShingle | Pei-Ran Lv Yang-Shuai Su Wei He Xiao-Yu Wang Hong Shi Xiao-Ning Zhang Bing Zhu Yu Kan Li-Zhen Chen Qiao-Feng Wu Shu-Guang Yu Xiang-Hong Jing Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats Neural Plasticity |
| title | Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats |
| title_full | Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats |
| title_fullStr | Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats |
| title_full_unstemmed | Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats |
| title_short | Electroacupuncture Alleviated Referral Hindpaw Hyperalgesia via Suppressing Spinal Long-Term Potentiation (LTP) in TNBS-Induced Colitis Rats |
| title_sort | electroacupuncture alleviated referral hindpaw hyperalgesia via suppressing spinal long term potentiation ltp in tnbs induced colitis rats |
| url | http://dx.doi.org/10.1155/2019/2098083 |
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